A                 Bacterial Inclusion Bodies (IBs)                                                  B
                    Stress conditions   Ins Sol
Recombinant                                                N       hFGF-2           H6         C
 expression
                        37 ºC, 3 h
                       IPTG: 1 mM

                    Artificial Inclusion Bodies (ArtIBs)
                                                               Pure soluble




                                                                                           precipitation
                    Mild conditions     Ins Sol                 hFGF-2-H6




                                                                                              100 %
                       20 ºC, O/N
                     IPTG: 0.1 mM                          Divalent cations


  C                                                                                                        D
                                                                                                           hFGF-2-    GFP-H6
    Intensity (%)




                                                                              Divalent cation                H6      (Control)
                                                                                   Zinc                     1:50      1:100

                                                                                 Calcium                    1:200     1:300

                                                                               Manganese                    1:400     1:500


                                          Mass (m/z)