A                                       Size: 6.8 nm
                                        PDI: 0.918
                                                       Size: 592.1 nm   Size: 1258 nm      Size: 1829 nm
                               30                      PDI: 1.000       PDI: 0.658         PDI: 0.634
                                                                                                                                                         hFGF-2 ArtIBs Zn
Volume (%)


                                                                                                                                                         Soluble hFGF-2
                               20                                                                                                                        hFGF-2 IBs
                                                                                                                                                         GFP ArtIBs Zn
                               10


                                0
                                    1                    10                 100                                             1000


B                                                               Size (nm)
                                                                                                                                            C




                                                                                                                                                                  ArtIBs
                                                                                                                                                     ArtIBs
                                          hFGF-2 IBs                           hFGF-2 ArtIBs Zn




                                                                                                                                                                  IBs
                                                                                                                                                     IBs
                                                                                                                                                           TGX       WB




                                                                                                                                                 25kDa

                                                                                                                                                 20kDa

                                                                                                                                                 15kDa




D
                                                                                        Fluorescence intensity (A.U)485nm




                                                          Mn2+                                                                                     hFGF-2 ArtIBs
Fluorescence intensity (A.U)




                                                          Ca2+ hFGF-2 ArtIBs
                                                          Zn2+
                                                          Soluble hFGF-2
                                                          Thio T (control)




                                                                                                                               Thio T Soluble    Ca2+      Mn2+   Zn2+
                                                       λ (nm)                                                                 (control) hFGF-2