A                                              Released protein
                                                     Days 1
                                                          L
                                                              3
                                                               L
                                                                    5
                                                                    L
                                                                         7
                                                                          L
                                                                                      B                        *
                                                                                                                      *   #
                                                                                                                     *




                                                                                      Proliferation (%)
                                  hFGF-2 ArtIBs                  18.1% 16.8% 45.4%

                                                     Mn2+
                                 Mn2+ Ca2+   Zn2+
                                                            29.6% 33.3% 40.9% 88.3%

                                 0d 7d 0d 7d 0d 7d                                                                            *
                                                     Ca2+
                        20 kDa
                                                                  44.6% 58.8% 97.1%

                                                     Zn2+
                                                                                                              Zn2+ Ca2+ Mn2+
  C
                                                                                                                   Tonset (°C)
Fluorescence intensity (A.U)




                                                                                                          Control 65
                                                                                                          Ca2+    70
                                                                                                          Zn2+    70
                                                                    CSM (nm)




                                         λ (nm)                                                      Temperature (°C)