AN                    HCP1        H6 C
                                                Pseudomonas aeruginosa HCP1 protein
                                                MAVDMFIKIGDVKGESKDKTHAEEIDVLAWSWGMSQSGSMHMGGGGGAGKVNVQDLS
                                                FTKYIDKSTPNLMMACSSGKHYPQAKLTIRKAGGENQVEYLIITLKEVLVSSVSTGGSGGEDR
                                                                                                                   B    M


                                                LTENVTLNFAQVQVDYQPQKADGAKDGGPVKYGWNIRQNVQAHHHHHH
                                                                                                                        75
                                                Phage PRD1 Major Capsid Protein (P3)
                                                MAQVQQLTPAQQAALRNQQAMAANLQARQIVLQQSYPVIQQVETQTFDPANRSVFDVT              50
                                                PANVGIVKGFLVKVTAAITNNHATEAVALTDFGPANLVQRVIYYDPDNQRHTETSGWHLHFV
 N                    MCP3        H6 C          NTAKQGAPFLSSMVTDSPIKYGDVMNVIDAPATIAAGATGELTMYYWVPLAYSETDLTGAVL          37
                                                ANVPQSKQRLKLEFANNNTAFAAVGANPLEAIYQGAGAADCEFEEISYTVYQSYLDQLPVG
                                                QNGYILPLIDLSTLYNLENSAQAGLTPNVDFVVQYANLYRYLSTIAVFDNGGSFNAGTDINYLS
                                                QRTANFSDTRKLDPKTWAAQTRRRIATDFPKGVYYCDNRDKPIYTLQYGNVGFVVNPKTVN           25
                                                QNARLLMGYEYFTSRTELVNAGTISTTHHHHHH
                                                                                                                        20
                                                Phage Lambda D protein
                  N       PLD     H6 C          MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSSRKLVAWDGTTDGAAV
                                                GILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAFAGTAISIVHHHHHH
                                                                                                                        15
                                                                                                                        10

C            30
                      Building Blocks        Nanoparticles
                                                                D                        Size                   Zp                      MW
                                                                         Sample                     PDI                      DNAbc
                                                                                        (nm)                   (mV)                    (KDa)
Volume (%)




             20                                                                        8.649 ±     0.467 ±    -6.38 ±        0.538 ±
                                                                      HCP1-H6                                                          18.101
                                                                                        0.148       0.014      0.355          0.021
                                                                                       23.780 ±    0.158 ±   -13.67 ±        0.535 ±
             10 RC (6-7
                                                                      MCP3-H6                                                          43.847
                                                                                        0.471       0.019     0.371           0.025
                 nm)
                                                                                       3.974 ±     0.330 ±   -13.93 ±        0.531 ±
                                                                      PLD-H6                                                           12.263
                                                                                        0.025       0.121     1.167           0.021
              0
                  1                     10        Size (nm) 100
E                      HCP1 (1Y12 PDB)                          MCP3 (1CJD PDB)                                PLD (1C5E PDB)




                        5.4 nm               8.9 nm                7.6 nm                 8.7 nm                   3.7 nm               5.7 nm