A            30
                             HCP1-H6

                             Building Blocks
                                            MCP3-H6         PLD-H6

                                                        Nanoparticles
                                                                                                                           B
                                              Zn2+   (0.2 mM)                            Size    8.649 nm ± 0.148            HCP1                 Bars size: 50 nm
Volume (%)

                                                                                         PDI     0.388 ± 0.036
             20
                                                                                                 Oligomerization

             10                                                                          Size    25.67 nm ± 0.182
                                                                                         PDI     0.182 ± 0.001

               0
                         1                       10                            100
             30
                              Building Blocks           Nanoparticles                                                        MCP3
                                                                                         Size    23.793 nm ± 1.445
Volume (%)




             20                                        Zn2+ (0.2 mM)                     PDI     0.158 ± 0.019

                                                                                                  Oligomerization

             10                                                                          Size    29.353 nm ± 0.278
                                                                                         PDI     0.201 ± 0.005


                0
                         1                       10                            100
             30                                                                                                               PLD
                             Building Blocks            Nanoparticles
                                                45 ºC
                                                                                         Size    3.974 nm ± 0.025
Volume (%)




             20                                                                          PDI     0.467 ± 0.014

                                                                                                 Oligomerization
             10                                                                          Size    23.59 nm ± 0.103
                                                                                         PDI     0.284 ± 0.013

                0

C                    1000
                         1                       10             Size (nm) 100
                                                                                                  D          1000
                                   HCP1-H6       MCP3-H6              PLD-H6
                                                                                           MPs




                                                                                                                                                                  MPs
                         100                                    * *                                               100
             Size (nm)




                                                                                                      Size (nm)




                                              * *                                                                                   *         *           *
                                                                                           NPs




                                                                                                                                                                  NPs

                             10                                                                                    10                    *
                                                                                           BBs




                                                                                                                                                                  BBs




                              1                                                                                     1
                                  Control     1 day RT 1 day 37 ºC             F/T x 4                                  Control   NaCl       EDTA     Imidazole
                                                        + OptiPro