A                T22-GFP-H6                        T22-GFP-H6 (LOOP)                     D

                 H6-GFP-T22                         GFP-H6




B
                         GFP-H6 – 6.5 nm
                        PDI 0.506 ± 0.033
                                                                 T22-GFP-H6(LOOP) – 6.5 nm
                                                                     PDI 0.522 ± 0.075        E
Volume (%)




                       H6-GFP-T22 – 5.6 nm                             T22-GFP-H6 – 11.7 nm                        100
                        PDI 0.543 ± 0.005                                PDI 0.486 ± 0.002
                                                                                                                    80




                                                                                              CELL VIABILITY (%)
                                                                                                                    60

C                     T22-GFP-H6 + EDTA – 5.6 nm
                                                   Size (nm)
                                                                                                                    40
                           PDI 0.595 ± 0.117                           T22-GFP-H6 – 11.7 nm                         20
                                                                         PDI 0.486 ± 0.002
    Volume (%)




                      T22-GFP-H6 + SDS – 5.6 nm
                          PDI 0.471 ± 0.015                                                                          0




                                                   Size (nm)