ee38a919dd8e71fd2ad0db13c27d9a61 animals_a2020v10p1898.pdf 04db07bb14b3e37eb62dfa3e3931f39b411fa393 animals_a2020v10p1898.pdf f8af1a92e7a330dd20d602326ed2bfadc5252ecacf4e2466f8b1d0b200f3e700 animals_a2020v10p1898.pdf Title: Welfare Benefits of Intradermal Vaccination of Piglets Subject: Vaccination is reported as a stressful and painful event for animals. This study investigated whether needle-free intradermal vaccination improves the welfare of weaned pigs through the reduction of stress and pain biomarkers and improvement of behavioural parameters compared to traditional intramuscular injection with a needle. A total of 339 weaned piglets were allocated to 3 treatment groups: Intradermal Application of Liquids (IDAL) pigs, vaccinated against Porcine Circovirus type 2 (PCV2) by means of intradermal vaccination using a needle-free device Porcilis® PCV ID; Intramuscular (IM) pigs vaccinated against PCV2 with Porcilis® PCV intramuscularly with a needle; CONTROL pigs were managed identically but did not receive any vaccine injection. At the time of the injection, the reaction of IDAL piglets was similar to control piglets, whereas a greater percentage of piglets vaccinated intramuscularly displayed high-pitch vocalizations (7% CONTROL, 7% IDAL, 32% IM) and retreat attempts (3% CONTROL, 7% IDAL, 39% IM). The day after vaccination, IDAL piglets did not differ from the control piglets for any of the behavioural variables studied through scan samplings. IM piglets showed a lower frequency of social negative interactions (p = 0.001) and rope manipulation (p = 0.04) compared to the CONTROL group. Resting postures did not differ between treatments. At 28 h post-vaccination, IDAL piglets presented lower blood C-reactive protein levels (CONTROL = 20 g/mL; IDAL = 39 g/mL; IM = 83 g/mL, p < 0.0001) and blood Haptoglobin (CONTROL = 1.8 mg/mL; IDAL = 1.9 mg/mL vs. IM = 3.1 mg/mL, p < 0.0001) compared to IM piglets. Salivary chromogranin A and alpha-amylase did not differ between treatment groups when measured 25 min post-vaccination. The method of vaccination did not affect the growth of the piglets or their rectal temperature. These results support that needle-free intradermal vaccination reduces vaccination-related pain in growing pigs. Keywords: intradermal vaccination; pig; behaviour; stress; welfare Author: Déborah Temple, Marta Jiménez, Damián Escribano, Gerard Martín-Valls, Ivan Díaz and Xavier Manteca Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Oct 16 12:23:37 2020 CEST ModDate: Fri Oct 16 12:23:37 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 260962 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AKCULY+URWPalladioL-Ital Type 1 Custom yes yes no 80 0 CJTOET+URWPalladioL-Bold Type 1 Custom yes yes no 81 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 82 0 IJBKBK+URWPalladioL-Roma Type 1 Custom yes yes no 83 0 FATHTC+Rpxr Type 1 Builtin yes yes no 84 0 TGMEOR+Rpcxr Type 1 Builtin yes yes no 85 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 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NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぅぴとどっちぬぜ〴ぜ〰こぴちぴづねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぁのどねちぬ〬ぜ〴ぜ〰えはふびどので〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぅへばづひどねづのぴちぬぜ〴ぜ〰ぐひはっづつふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰あづとちぶどはふひちぬぜ〴ぜ〰きづちびふひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰えづちぬぴとぜ〴ぜ〰きづちびふひづびぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぅぴとどっちぬぜ〴ぜ〰こぴちぴづねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぁのどねちぬ〬ぜ〴ぜ〰えはふびどので〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぅへばづひどねづのぴちぬぜ〴ぜ〰ぐひはっづつふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰あづとちぶどはふひちぬぜ〴ぜ〰きづちびふひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰えづちぬぴとぜ〴ぜ〰きづちびふひづびぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぁのどねちぬ〬ぜ〴ぜ〰えはふびどので〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぅへばづひどねづのぴちぬぜ〴ぜ〰ぐひはっづつふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰あづとちぶどはふひちぬぜ〴ぜ〰きづちびふひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰えづちぬぴとぜ〴ぜ〰きづちびふひづびぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: あづとちぶどはふひちぬぜ〴ぜ〰きづちびふひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰えづちぬぴとぜ〴ぜ〰きづちびふひづびぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: えづちぬぴとぜ〴ぜ〰きづちびふひづびぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こちねばぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰あづとちぶどはふひちぬぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰えづちぬぴとぜ〴ぜ〰いちぴちぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: あづとちぶどはふひちぬぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぐとべびどはぬはでどっちぬぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰えづちぬぴとぜ〴ぜ〰いちぴちぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぐとべびどはぬはでどっちぬぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰えづちぬぴとぜ〴ぜ〰いちぴちぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: えづちぬぴとぜ〴ぜ〰いちぴちぜ〴ぜ〰ちのつぜ〴ぜ〰あはつべぜ〴ぜ〰しづどでとぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ 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