83c8839d021406de52bdfb20e5f1de32 animals_a2020v10n11p1993_2.pdf 90151b27f347fe1dca1ebb90342a020cdf404754 animals_a2020v10n11p1993_2.pdf 714dcb063afa5b20ba7e9c9301405289d9721d2bce40b39b3e85926f71d3188c animals_a2020v10n11p1993_2.pdf Title: Optimization of CASA-Mot Analysis of Donkey Sperm: Optimum Frame Rate and Values of Kinematic Variables for Different Counting Chamber and Fields Subject: In order to optimize the donkey sperm motility analysis by the CASA (Computer Assisted Sperm Analysis)-Mot system, twelve ejaculates were collected from six jackasses. Capillary loaded chamber (CLC), ISAS®D4C depths 10 and 20 m, ISAS®D4C Leja 20 and drop displacement chamber (DDC), Spermtrack® (Spk) depths 10 and 20 m were used. Sperm kinematic variables were evaluated using each chamber and a high-resolution camera capable of capturing a maximum of 500 frames/second (fps). The optimum frame rate (OFR) (defined according to curvilinear velocity—VCL) was dependent on chamber type. The highest OFR obtained was 278.46 fps by Spk20. Values for VCL, straight-line velocity (VSL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were high in DDC and 10 m depth. In both DDC 10 and 20 m, the sperm velocities (VCL, VSL, VAP) and ALH values decreased significantly from the centre to the edges, while Wobble and BCF increased. No defined behavior was observed along the CLC. However, all the kinematic variables had a higher value in a highly concentrated sample, in both chamber types. In conclusion, analyzing a minimum of nine fields at 250 fps from the centre to the edges in Spk10 chamber using a dilution of 30 106 sperm/mL offers the best choice for donkey computerised sperm motility analysis. Keywords: frame rate; drop displacement chambers; capillary loaded chambers; chamber depth; field; sperm dilution Author: Sabrina Gacem, Jaime Catalán, Anthony Valverde, Carles Soler and Jordi Miró Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Oct 29 13:54:32 2020 CET ModDate: Thu Oct 29 13:54:32 2020 CET Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1455303 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- NILHIY+URWPalladioL-Ital Type 1 Custom yes yes no 92 0 XOIXRK+URWPalladioL-Bold Type 1 Custom yes yes no 93 0 LZDGTK+Rpxb Type 1 Builtin yes yes no 94 0 ENXZUH+VnURWPalladioL-Bold Type 1 Custom yes yes no 95 0 SDNBJG+URWPalladioL-Roma Type 1 Custom yes yes no 96 0 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