ff23efad08a99125572f6dc502909f5c animals_a2020v10n12p2194.pdf d9f239f95117abde6015a20162ad7451045188cf animals_a2020v10n12p2194.pdf 31e76c20aa82705b48f33936d8f23909e9710ef4a54258813a67714d798b12de animals_a2020v10n12p2194.pdf Title: Assessing the Diversity and Population Substructure of Sarda Breed Bucks by Using Mtdna and Y-Chromosome Markers Subject: A sample of 146 Sarda bucks from eight subregions of Sardinia, Italy (Nuorese, Barbagia, Baronia, Ogliastra, Sarrabus, Guspinese, Iglesiente, Sulcis) were characterized for Y-chromosome and mtDNA markers to assess the levels of population substructure. Five polymorphic loci (SRY, AMELY, ZFY, and DDX3Y) on the Y-chromosome were genotyped. The control region of mtDNA was sequenced as a source of complementary information. Analysis of Y-chromosome data revealed the segregation of 5 haplotypes: Y1A (66.43%), Y2 (28.57%), Y1C (3.57%), Y1B1 (0.71%), and Y1B2 (0.71%). High levels of Y-chromosome diversity were observed in populations from Southwest Sardinia. The FST values based on Y-chromosome and mtDNA data were low, although a paternal genetic differentiation was observed when comparing the Nuorese and Barbagia populations (Central Sardinia) with the Sulcis, Iglesiente, and Sarrabus populations (Southern Sardinia). AMOVA analysis supported the lack of population substructure. These results suggest the occurrence of a historical and extensive gene flow between Sarda goat populations from different locations of Sardinia, despite the fact that this island is covered by several large mountain ranges. Introgression with foreign caprine breeds in order to improve milk production might have also contributed to avoiding the genetic differentiation amongst Sarda populations. Keywords: Y-chromosome; mitochondrial DNA; buck; population structuring; goat; SRY; AMELY; ZFY; DDX3Y Author: Maria Luisa Dettori, Elena Petretto, Michele Pazzola, Oriol Vidal, Marcel Amills and Giuseppe Massimo Vacca Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Tue Nov 24 07:37:37 2020 CET ModDate: Tue Nov 24 07:37:37 2020 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1343969 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QTAYQD+URWPalladioL-Ital Type 1 Custom yes yes no 82 0 UIJREB+URWPalladioL-Bold Type 1 Custom yes yes no 83 0 CEWEHE+URWPalladioL-Roma Type 1 Custom yes yes no 84 0 WTTHMN+VnURWPalladioL Type 1 Custom yes yes no 85 0 OFLXAT+Rpxr Type 1 Builtin yes yes no 86 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C 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false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぅぴとどっびぜ〴ぜ〰ぁばばひはぶちぬぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰ちのつぜ〴ぜ〰いぎぁぜ〴ぜ〰ぐふひどてどっちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰す〭ぃとひはねはびはねづぜ〴ぜ〰ぇづのはぴべばどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぃはねばふぴちぴどはのちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぅぴとどっびぜ〴ぜ〰ぁばばひはぶちぬぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰ちのつぜ〴ぜ〰いぎぁぜ〴ぜ〰ぐふひどてどっちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰す〭ぃとひはねはびはねづぜ〴ぜ〰ぇづのはぴべばどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぃはねばふぴちぴどはのちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こちねばぬどのでぜ〴ぜ〰ちのつぜ〴ぜ〰いぎぁぜ〴ぜ〰ぐふひどてどっちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰す〭ぃとひはねはびはねづぜ〴ぜ〰ぇづのはぴべばどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぃはねばふぴちぴどはのちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: す〭ぃとひはねはびはねづぜ〴ぜ〰ぇづのはぴべばどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぃはねばふぴちぴどはのちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぃはねばふぴちぴどはのちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぃはねばふぴちぴどはのちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰す〭ぃとひはねはびはねづぜ〴ぜ〰きちひにづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: す〭ぃとひはねはびはねづぜ〴ぜ〰きちひにづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: きどぴはっとはのつひどちぬぜ〴ぜ〰いぎぁぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐちぴぴづひのびぜ〴ぜ〰はてぜ〴ぜ〰す〭ぃとひはねはびはねづぜ〴ぜ〰ちのつぜ〴ぜ〰きどぴはっとはのつひどちぬぜ〴ぜ〰いどぶづひびどぴべぜ〴ぜ〰どのぜ〴ぜ〰こちひつちぜ〴ぜ〰あふっにびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰がちっにぜ〴ぜ〰はてぜ〴ぜ〰ぐはばふぬちぴどはのぜ〴ぜ〰こふぢびぴひふっぴふひづぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰こちひつちぜ〴ぜ〰あひづづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Children: Item: Title: ぐちぴぴづひのびぜ〴ぜ〰はてぜ〴ぜ〰す〭ぃとひはねはびはねづぜ〴ぜ〰ちのつぜ〴ぜ〰きどぴはっとはのつひどちぬぜ〴ぜ〰いどぶづひびどぴべぜ〴ぜ〰どのぜ〴ぜ〰こちひつちぜ〴ぜ〰あふっにびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰がちっにぜ〴ぜ〰はてぜ〴ぜ〰ぐはばふぬちぴどはのぜ〴ぜ〰こふぢびぴひふっぴふひづぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰こちひつちぜ〴ぜ〰あひづづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: がちっにぜ〴ぜ〰はてぜ〴ぜ〰ぐはばふぬちぴどはのぜ〴ぜ〰こふぢびぴひふっぴふひづぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰こちひつちぜ〴ぜ〰あひづづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QTAYQD+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: QTAYQD+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: UIJREB+URWPalladioL-Bold FontSubset: true FirstChar: 3 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