57f3affd28049b5f50cdda4bbf654350 vaccines_a2020v8n4p751.pdf 003289beea9b9180dcaa9a471215478e242674bc vaccines_a2020v8n4p751.pdf 0a959b879caeb190d3227dc43682b09ff5f48cb764cb30b067fe056dbbeaf1ea vaccines_a2020v8n4p751.pdf Title: Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year Subject: Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette–Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN- to determine the presence of antigen-specific cells secreting IFN-. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN- responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN- production and a greater percentage of antigen-specific CD4+CD45RO+IFN+ and CD4-CD45RO+IFN+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae. Keywords: BCG; revaccination; duration of immunity; goat; tuberculosis; vaccine; diagnosis; interferon gamma; antigen-specific memory T-cells Author: Claudia Arrieta-Villegas, Enric Vidal, Maite Martín, Judit Verdés, Xavier Moll, Yvonne Espada, Mahavir Singh, Bernardo Villarreal-Ramos, Mariano Domingo and Bernat Pérez de Val Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Sun Dec 13 12:21:52 2020 CET ModDate: Wed Dec 16 06:25:03 2020 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 21543943 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 CIQLLF+VnURWPalladioL Type 1 Custom yes yes no 15 0 VHEQAI+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 UIILMT+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 QQBJIJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 WTSVZQ+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 FATHTC+Rpxr Type 1 Builtin yes yes no 36 0 TKEEVY+Pxsy Type 1 Builtin yes yes no 40 0 IHITRA+EURM10 Type 1 Builtin yes yes no 44 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 71 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 75 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 79 0 ESSRQF+VnURWPalladioL-Italic Type 1 Custom yes yes no 86 0 YICMPF+Rpcxr Type 1 Builtin yes yes no 90 0 DEYHCE+Rpxmi Type 1 Builtin yes yes no 97 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 118 0 GTPPSH+Rpxi Type 1 Builtin yes yes no 144 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:23:38 CEST RepresentationInformation: vaccines_a2020v8n4p751.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:40:00 CEST Size: 21543943 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 369 FreeObjects: 13 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals, Vaccination Schedule and Experimental Infection Destination: subsection.2.1 Item: Title: Ethics Statement Destination: subsection.2.2 Item: Title: In Vitro IFN- Release Assay (IGRA) Destination: subsection.2.3 Item: Title: Flow Cytometry and Intracellular IFN- Staining Destination: subsection.2.4 Item: Title: Skin Tests Destination: subsection.2.5 Item: Title: Post-Mortem Examination Destination: subsection.2.6 Item: Title: M. caprae DNA Burden Assessment by qPCR Destination: subsection.2.7 Item: Title: Data Analysis Destination: subsection.2.8 Item: Title: Results Destination: section.3 Children: Item: Title: Immunological Responses after Vaccination and Revaccination Destination: subsection.3.1 Item: Title: IFN- Responses After M. caprae Challenge Destination: subsection.3.2 Item: Title: Clinical Signs and Body Condition Post-Challenge Destination: subsection.3.3 Item: Title: Post-Mortem Findings Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year Author: Claudia Arrieta-Villegas, Enric Vidal, Maite Martín, Judit Verdés, Xavier Moll, Yvonne Espada, Mahavir Singh, Bernardo Villarreal-Ramos, Mariano Domingo and Bernat Pérez de Val Subject: Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN- to determine the presence of antigen-specific cells secreting IFN-. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN- responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN- production and a greater percentage of antigen-specific CD4+CD45RO+IFN+ and CD4-CD45RO+IFN+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae. 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