dac9baf12f40805d426f8118be0e6cb2 diagnostics_a2020v10n10p845.pdf 9538ee071769ab192cd08b6b09ccb7eb94b05274 diagnostics_a2020v10n10p845.pdf 3226e84cc898a2c4924d2a6aeb9f0559de73a1ac5ffcbcf867b71922e5ba0a9b diagnostics_a2020v10n10p845.pdf Title: Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor Subject: Infections by multidrug-resistant bacteria are becoming a major healthcare emergence with millions of reported cases every year and an increasing incidence of deaths. An advanced diagnostic platform able to directly detect and identify antimicrobial resistance in a faster way than conventional techniques could help in the adoption of early and accurate therapeutic interventions, limiting the actual negative impact on patient outcomes. With this objective, we have developed a new biosensor methodology using an ultrasensitive nanophotonic bimodal waveguide interferometer (BiMW), which allows a rapid and direct detection, without amplification, of two prevalent and clinically relevant Gram-negative antimicrobial resistance encoding sequences: the extended-spectrum betalactamase-encoding gene blaCTX-M-15 and the carbapenemase-encoding gene blaNDM-5 We demonstrate the extreme sensitivity and specificity of our biosensor methodology for the detection of both gene sequences. Our results show that the BiMW biosensor can be employed as an ultrasensitive (attomolar level) and specific diagnostic tool for rapidly (less than 30 min) identifying drug resistance. The BiMW nanobiosensor holds great promise as a powerful tool for the control and management of healthcare-associated infections by multidrug-resistant bacteria. Keywords: nanophotonic biosensor; multidrug-resistance; Escherichia coli; bimodal waveguide interferometer; NDM; CTX-M Author: Jesús Maldonado, Ana Belén González-Guerrero, Adrián Fernández-Gavela, Juan José González-López and Laura M. 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2019-04-17) Date: 2021-02-17 02:20:48 CET RepresentationInformation: diagnostics_a2020v10n10p845.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-02-16 13:37:01 CET Size: 2087077 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぃとづねどっちぬぜ〴ぜ〰げづちでづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰いづぶどっづぜ〴ぜ〰ちのつぜ〴ぜ〰ぅへばづひどねづのぴちぬぜ〴ぜ〰こづぴ〭さばぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰きどっひはてぬふどつどっぜ〴ぜ〰こべびぴづねぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰こふひてちっづぜ〴ぜ〰ぃぬづちのどので〬ぜ〴ぜ〰ぁっぴどぶちぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰あどはてふのっぴどはのちぬどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐひはぢづびぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぜ〴ぜ〰ぃふぬぴふひづ〬ぜ〴ぜ〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぃげぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぃとづねどっちぬぜ〴ぜ〰げづちでづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰いづぶどっづぜ〴ぜ〰ちのつぜ〴ぜ〰ぅへばづひどねづのぴちぬぜ〴ぜ〰こづぴ〭さばぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰きどっひはてぬふどつどっぜ〴ぜ〰こべびぴづねぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰こふひてちっづぜ〴ぜ〰ぃぬづちのどので〬ぜ〴ぜ〰ぁっぴどぶちぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰あどはてふのっぴどはのちぬどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐひはぢづびぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぜ〴ぜ〰ぃふぬぴふひづ〬ぜ〴ぜ〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぃげぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: あどきしぜ〴ぜ〰いづぶどっづぜ〴ぜ〰ちのつぜ〴ぜ〰ぅへばづひどねづのぴちぬぜ〴ぜ〰こづぴ〭さばぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰きどっひはてぬふどつどっぜ〴ぜ〰こべびぴづねぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰こふひてちっづぜ〴ぜ〰ぃぬづちのどので〬ぜ〴ぜ〰ぁっぴどぶちぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰あどはてふのっぴどはのちぬどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐひはぢづびぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぜ〴ぜ〰ぃふぬぴふひづ〬ぜ〴ぜ〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぃげぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: きどっひはてぬふどつどっぜ〴ぜ〰こべびぴづねぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰こふひてちっづぜ〴ぜ〰ぃぬづちのどので〬ぜ〴ぜ〰ぁっぴどぶちぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰あどはてふのっぴどはのちぬどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐひはぢづびぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぜ〴ぜ〰ぃふぬぴふひづ〬ぜ〴ぜ〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぃげぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: あどきしぜ〴ぜ〰こふひてちっづぜ〴ぜ〰ぃぬづちのどので〬ぜ〴ぜ〰ぁっぴどぶちぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰あどはてふのっぴどはのちぬどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐひはぢづびぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぜ〴ぜ〰ぃふぬぴふひづ〬ぜ〴ぜ〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぃげぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぐひはぢづびぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぜ〴ぜ〰ぃふぬぴふひづ〬ぜ〴ぜ〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぃげぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: あちっぴづひどちぜ〴ぜ〰ぃふぬぴふひづ〬ぜ〴ぜ〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぃげぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: あどきしぜ〴ぜ〰ぁびびちべぜ〴ぜ〰いづぶづぬはばねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴ぜ〰ちのつぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰あどはちびびちべぜ〴ぜ〰こぴひちぴづでべぜ〴ぜ〰ちのつぜ〴ぜ〰ぅぶちぬふちぴどはのぜ〴ぜ〰ぷどぴとぜ〴ぜ〰こべのぴとづぴどっぜ〴ぜ〰いぎぁぜ〴ぜ〰ごちひでづぴぜ〴ぜ〰こづぱふづのっづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぅぶちぬふちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇづのづびぜ〴ぜ〰ぅのっはつどのでぜ〴ぜ〰あづぴちぬちっぴちねちびづぜ〴ぜ〰ぃごじ〭き〭〱〵ぜ〴ぜ〰ちのつぜ〴ぜ〰ぃちひぢちばづのづねちびづぜ〴ぜ〰ぎいき〭〵ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Children: Item: Title: あどはちびびちべぜ〴ぜ〰こぴひちぴづでべぜ〴ぜ〰ちのつぜ〴ぜ〰ぅぶちぬふちぴどはのぜ〴ぜ〰ぷどぴとぜ〴ぜ〰こべのぴとづぴどっぜ〴ぜ〰いぎぁぜ〴ぜ〰ごちひでづぴぜ〴ぜ〰こづぱふづのっづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぅぶちぬふちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇづのづびぜ〴ぜ〰ぅのっはつどのでぜ〴ぜ〰あづぴちぬちっぴちねちびづぜ〴ぜ〰ぃごじ〭き〭〱〵ぜ〴ぜ〰ちのつぜ〴ぜ〰ぃちひぢちばづのづねちびづぜ〴ぜ〰ぎいき〭〵ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Item: Title: ぅぶちぬふちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇづのづびぜ〴ぜ〰ぅのっはつどのでぜ〴ぜ〰あづぴちぬちっぴちねちびづぜ〴ぜ〰ぃごじ〭き〭〱〵ぜ〴ぜ〰ちのつぜ〴ぜ〰ぃちひぢちばづのづねちびづぜ〴ぜ〰ぎいき〭〵ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Children: Item: Title: あづぴちぬちっぴちねちびづぜ〴ぜ〰ぃごじ〭き〭〱〵ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸲ Item: Title: ぃちひぢちばづのづねちびづぜ〴ぜ〰ぎいき〭〵ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: OFYUNO+URWPalladioL-Bold 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