386bad24b089a9762c010201ebb6c494 241181.pdf 17b4a6cef5e8c2b6b917b14a2881f2fcb787d39f 241181.pdf 33a71196eff171450a3f1db8d27247d44f642e600e356ab0aad36864d13fcf09 241181.pdf Title: Immune Cell Subtypes and Cytokines in Lung Tumor Microenvironment: Influence of COPD Subject: Background: The immune microenvironment plays a role in tumorigenesis. Chronic Obstructive Pulmonary Disease (COPD) is an independent risk factor for lung cancer (LC). We hypothesized that immune profile characterized by T regulatory (Treg), natural killer (NK), and plasma cells, as well as interleukin (IL)-10 and interferon-gamma, may differ within tumors of LC patients with/without COPD. Methods: Treg (anti-CD3 and anti-forkhead boxP3 antibodies), NK (anti-NCR1 antibody), IgG (anti-CD138-IgG antibody), IgA (anti-CD138-IgA antibody) using immunohistochemistry, and both IL-10 and interferon-gamma (ELISA) were quantified in tumor and non-tumor specimens (thoracotomy for lung tumor resection) from 33 LC–COPD patients and 20 LC-only patients. Results: Immune profile in tumor versus non-tumor specimens: Treg cell counts significantly increased in tumors of both LC and LC–COPD patients, while in tumors of the latter group, IgG-secreting plasma cells significantly decreased and IL-10 increased. No significant differences were seen in levels of NK cells, IgA-secreting cells, IgA/IgG, or interferon-gamma. Immune profile in tumors of LC–COPD versus LC: No significant differences were observed in tumors between LC–COPD and LC patients for any study marker. Conclusions: Immune cell subtypes and cytokines are differentially expressed in lung tumors, and the presence of COPD elicited a decline in IgG-secreting plasma cell levels but not in other cell types. Keywords: lung cancer; COPD; T regulatory cells; natural killer cells: immunoglobulin-secreting plasma cells; immune tumor microenvironment; IL-10 and interferon-gamma Author: Jun Tang, Daniel Ramis-Cabrer, Víctor Curull, Xuejie Wang, Liyun Qin, Mercé Mateu-Jiménez, Xavier Duran, Lara Pijuan, Alberto Rodríguez-Fuster, Rafael Aguiló Espases and Esther Barreiro Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Wed May 13 12:29:29 2020 CEST ModDate: Wed May 13 12:29:29 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5840584 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FFKFKN+URWPalladioL-Ital Type 1 Custom yes yes no 123 0 LWJGHZ+URWPalladioL-Bold Type 1 Custom yes yes no 124 0 XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes no 125 0 FEDLBE+URWPalladioL-Roma Type 1 Custom yes yes no 126 0 AEPYJE+VnURWPalladioL Type 1 Custom yes yes no 127 0 QLMOVM+Rpxr Type 1 Builtin yes yes no 128 0 LCJEVJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 134 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 151 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 152 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 153 0 ETUHEA+Pxsy Type 1 Builtin yes yes no 231 0 BBSDEQ+EURM10 Type 1 Builtin yes yes no 243 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 260 0 VUPETJ+Rpxb Type 1 Builtin yes yes no 261 0 JDGHDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 276 0 JDGHFL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 277 0 JDGHFM+PalatinoLinotype CID TrueType Identity-H yes yes yes 278 0 JDGHFN+PalatinoLinotype TrueType WinAnsi yes yes no 279 0 JDGIDJ+TimesNewRoman CID TrueType Identity-H yes yes yes 280 0 JDGHDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 319 0 JDGHFL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 320 0 JDGHFM+PalatinoLinotype CID TrueType Identity-H yes yes yes 321 0 JDGHFN+PalatinoLinotype TrueType WinAnsi yes yes no 322 0 JDGIDJ+TimesNewRoman CID TrueType Identity-H yes yes yes 323 0 JDGHDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 356 0 JDGHFL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 357 0 JDGHFM+PalatinoLinotype CID TrueType Identity-H yes yes yes 358 0 JDGHFN+PalatinoLinotype TrueType WinAnsi yes yes no 359 0 JDGIDJ+TimesNewRoman CID TrueType Identity-H yes yes yes 360 0 JDGHDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 388 0 JDGHFL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 389 0 JDGHFN+PalatinoLinotype TrueType WinAnsi yes yes no 390 0 JDGIDJ+TimesNewRoman CID TrueType Identity-H yes yes yes 391 0 JDGHDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 425 0 JDGHFL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 426 0 JDGHFM+PalatinoLinotype CID TrueType Identity-H yes yes yes 427 0 JDGHFN+PalatinoLinotype TrueType WinAnsi yes yes no 428 0 JDGIDJ+TimesNewRoman CID TrueType Identity-H yes yes yes 429 0 GBLQZB+Rpxi Type 1 Builtin yes yes no 569 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 586 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-09-03 02:32:12 CEST RepresentationInformation: 241181.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-09-02 18:00:05 CEST Size: 5840584 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰こぴふつべぜ〴ぜ〰いづびどでのぜ〴ぜ〰ちのつぜ〴ぜ〰ぅぴとどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぃぬどのどっちぬぜ〴ぜ〰ぁびびづびびねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぐひづびづひぶちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごひづでぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃべぴはにどのづびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰ごどびびふづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: こぴふつべぜ〴ぜ〰いづびどでのぜ〴ぜ〰ちのつぜ〴ぜ〰ぅぴとどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぃぬどのどっちぬぜ〴ぜ〰ぁびびづびびねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぐひづびづひぶちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごひづでぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃべぴはにどのづびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰ごどびびふづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃぬどのどっちぬぜ〴ぜ〰ぁびびづびびねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぐひづびづひぶちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごひづでぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃべぴはにどのづびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰ごどびびふづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぐひづびづひぶちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごひづでぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃべぴはにどのづびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰ごどびびふづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごひづでぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰ちのつぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃべぴはにどのづびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰ごどびびふづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃべぴはにどのづびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰ごどびびふづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃべぴはにどのづびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰ごどびびふづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぃぬどのどっちぬぜ〴ぜ〰ぃとちひちっぴづひどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ごひづでぜ〴ぜ〰ちのつぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Children: Item: Title: ぃぬどのどっちぬぜ〴ぜ〰ぃとちひちっぴづひどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ごひづでぜ〴ぜ〰ちのつぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Item: Title: ごひづでぜ〴ぜ〰ちのつぜ〴ぜ〰ぎかぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Children: Item: Title: いどててづひづのっづびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰がぃぜ〲㍜〰ぃくぐいぜ〴ぜ〰ちのつぜ〴ぜ〰がぃぜ〴ぜ〰どのぜ〴ぜ〰づどぴとづひぜ〴ぜ〰ごふねはひぜ〴ぜ〰がづびどはのびぜ〴ぜ〰はひぜ〴ぜ〰ぎはの〭ごふねはひぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸲ Item: Title: いどててづひづのっづびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰ごふねはひぜ〴ぜ〰ちのつぜ〴ぜ〰ぎはの〭ごふねはひぜ〴ぜ〰ぐちひづのっとべねちぜ〴ぜ〰どのぜ〴ぜ〰がぃぜ〲㍜〰ぃくぐいぜ〴ぜ〰ちのつぜ〴ぜ〰がぃぜ〴ぜ〰ぐちぴどづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぉでぇぜ〴ぜ〰ちのつぜ〴ぜ〰ぉでぁぜ〴ぜ〰こづっひづぴどのでぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸳⸱ Item: Title: ぉでぇぜ〴ぜ〰ちのつぜ〴ぜ〰ぉでぁぜ〴ぜ〰こづっひづぴどのでぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸳⸱ Children: Item: Title: いどててづひづのっづびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰がぃぜ〲㍜〰ぃくぐいぜ〴ぜ〰ちのつぜ〴ぜ〰がぃぜ〴ぜ〰どのぜ〴ぜ〰づどぴとづひぜ〴ぜ〰ごふねはひぜ〴ぜ〰がづびどはのびぜ〴ぜ〰はひぜ〴ぜ〰ぎはの〭ごふねはひぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸳⸲ Item: Title: いどててづひづのっづびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰ごふねはひぜ〴ぜ〰ちのつぜ〴ぜ〰ぎはの〭ごふねはひぜ〴ぜ〰ぐちひづのっとべねちぜ〴ぜ〰どのぜ〴ぜ〰がぃぜ〲㍜〰ぃくぐいぜ〴ぜ〰ちのつぜ〴ぜ〰がぃぜ〴ぜ〰ぐちぴどづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづびぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸴⸱ Item: Title: ぃべぴはにどのづびぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰がふのでぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸴⸱ Children: Item: Title: いどててづひづのっづびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰がぃぜ〲㍜〰ぃくぐいぜ〴ぜ〰ちのつぜ〴ぜ〰がぃぜ〴ぜ〰どのぜ〴ぜ〰づどぴとづひぜ〴ぜ〰ごふねはひぜ〴ぜ〰がづびどはのびぜ〴ぜ〰はひぜ〴ぜ〰ぎはの〭ごふねはひぜ〴ぜ〰こばづっどねづのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸴⸲ Item: Title: いどててづひづのっづびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰ごふねはひぜ〴ぜ〰ちのつぜ〴ぜ〰ぎはの〭ごふねはひぜ〴ぜ〰ぐちひづのっとべねちぜ〴ぜ〰どのぜ〴ぜ〰がぃぜ〲㍜〰ぃくぐいぜ〴ぜ〰ちのつぜ〴ぜ〰がぃぜ〴ぜ〰ぐちぴどづのぴび〮ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QLMOVM+Rpxr FontSubset: true FirstChar: 11 LastChar: 62 FontDescriptor: FontName: QLMOVM+Rpxr Flags: Symbolic FontBBox: -40, -283, 904, 728 FontFile: true Font: BaseFont: BBSDEQ+EURM10 FontSubset: true FirstChar: 12 LastChar: 22 FontDescriptor: FontName: BBSDEQ+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: URYLRZ+URWPalladioL-BoldItal FontSubset: true FirstChar: 110 LastChar: 110 FontDescriptor: FontName: URYLRZ+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: VUPETJ+Rpxb FontSubset: true FirstChar: 61 LastChar: 61 FontDescriptor: FontName: VUPETJ+Rpxb Flags: Symbolic FontBBox: -57, -266, 932, 729 FontFile: true Font: BaseFont: ETUHEA+Pxsy FontSubset: true FirstChar: 0 LastChar: 20 FontDescriptor: FontName: ETUHEA+Pxsy Flags: Symbolic FontBBox: -21, -994, 1292, 912 FontFile: true Font: BaseFont: GBLQZB+Rpxi FontSubset: true FirstChar: 11 LastChar: 11 FontDescriptor: FontName: GBLQZB+Rpxi Flags: Symbolic FontBBox: -162, -280, 856, 733 FontFile: true Font: BaseFont: VSHNAE+Rpcxr FontSubset: true FirstChar: 169 LastChar: 169 FontDescriptor: FontName: VSHNAE+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: FFKFKN+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 121 FontDescriptor: FontName: FFKFKN+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: LWJGHZ+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: LWJGHZ+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XWOCXD+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 226 FontDescriptor: FontName: XWOCXD+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: FEDLBE+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: FEDLBE+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: AEPYJE+VnURWPalladioL FontSubset: true FirstChar: 128 LastChar: 226 FontDescriptor: FontName: AEPYJE+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 75, 757, 193, 793 Annotation: Subtype: Link Rect: 474, 757, 519, 793 Annotation: Subtype: Link Rect: 237, 661, 248, 675 Annotation: Subtype: Link Rect: 447, 648, 459, 662 Annotation: Subtype: Link Rect: 466, 415, 518, 434 Annotation: Subtype: Link Rect: 443, 84, 450, 93 ActionDest: 496 Annotation: Subtype: Link Rect: 453, 84, 460, 93 ActionDest: 496 Annotation: Subtype: Link Rect: 383, 71, 390, 80 ActionDest: 496 Annotation: Subtype: Link Rect: 394, 71, 400, 80 ActionDest: 496 Annotation: Subtype: Link Rect: 168, 38, 256, 50 Annotation: Subtype: Link Rect: 404, 38, 519, 50 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 333, 743, 340, 752 ActionDest: 496 Annotation: Subtype: Link Rect: 343, 743, 355, 752 ActionDest: 535 Annotation: Subtype: Link Rect: 298, 729, 310, 738 ActionDest: 535 Annotation: Subtype: Link Rect: 313, 729, 325, 738 ActionDest: 535 Annotation: Subtype: Link Rect: 207, 715, 219, 725 ActionDest: 535 Annotation: Subtype: Link Rect: 219, 715, 231, 725 ActionDest: 535 Annotation: Subtype: Link Rect: 353, 661, 360, 671 ActionDest: 496 Annotation: Subtype: Link Rect: 360, 661, 372, 671 ActionDest: 535 Annotation: Subtype: Link Rect: 391, 634, 398, 644 ActionDest: 496 Annotation: Subtype: Link Rect: 399, 634, 411, 644 ActionDest: 535 Annotation: Subtype: Link Rect: 298, 607, 310, 617 ActionDest: 535 Annotation: Subtype: Link Rect: 311, 607, 323, 617 ActionDest: 535 Annotation: Subtype: Link Rect: 194, 594, 205, 603 ActionDest: 535 Annotation: Subtype: Link Rect: 206, 594, 218, 603 ActionDest: 535 Annotation: Subtype: Link Rect: 430, 580, 442, 590 ActionDest: 535 Annotation: Subtype: Link Rect: 443, 580, 454, 590 ActionDest: 535 Annotation: Subtype: Link Rect: 205, 553, 217, 562 ActionDest: 535 Annotation: Subtype: Link Rect: 218, 553, 229, 562 ActionDest: 535 Annotation: Subtype: Link Rect: 445, 499, 457, 508 ActionDest: 535 Annotation: Subtype: Link Rect: 457, 499, 469, 508 ActionDest: 535 Annotation: Subtype: Link Rect: 328, 472, 340, 481 ActionDest: 535 Annotation: Subtype: Link Rect: 358, 459, 370, 468 ActionDest: 535 Annotation: Subtype: Link Rect: 278, 432, 290, 441 ActionDest: 535 Annotation: Subtype: Link Rect: 293, 432, 305, 441 ActionDest: 564 Annotation: Subtype: Link Rect: 121, 378, 133, 387 ActionDest: 564 Annotation: Subtype: Link Rect: 437, 204, 449, 213 ActionDest: 564 Annotation: Subtype: Link Rect: 460, 82, 472, 91 ActionDest: 564 Annotation: Subtype: Link Rect: 473, 82, 485, 91 ActionDest: 564 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 138, 729, 150, 738 ActionDest: 564 Annotation: Subtype: Link Rect: 448, 702, 455, 711 ActionDest: 496 Annotation: Subtype: Link Rect: 455, 702, 467, 711 ActionDest: 564 Annotation: Subtype: Link Rect: 129, 538, 136, 547 ActionDest: 496 Annotation: Subtype: Link Rect: 136, 538, 148, 547 ActionDest: 564 Annotation: Subtype: Link Rect: 442, 441, 449, 450 ActionDest: 496 Annotation: Subtype: Link Rect: 452, 441, 459, 450 ActionDest: 496 Annotation: Subtype: Link Rect: 462, 304, 469, 313 ActionDest: 496 Annotation: Subtype: Link Rect: 434, 115, 446, 124 ActionDest: 564 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 459, 403, 466, 412 ActionDest: 496 Annotation: Subtype: Link Rect: 371, 308, 383, 317 ActionDest: 564 Annotation: Subtype: Link Rect: 383, 308, 395, 317 ActionDest: 564 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 508, 696, 515, 705 ActionDest: 496 Annotation: Subtype: Link Rect: 456, 461, 463, 473 ActionDest: 257 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 252, 109, 258, 121 ActionDest: 257 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 419, 726, 426, 738 ActionDest: 257 Annotation: Subtype: Link Rect: 160, 699, 167, 711 ActionDest: 257 Annotation: Subtype: Link Rect: 478, 539, 485, 551 ActionDest: 267 Annotation: Subtype: Link Rect: 508, 539, 515, 551 ActionDest: 312 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 337, 370, 343, 382 ActionDest: 267 Annotation: Subtype: Link Rect: 255, 343, 263, 355 ActionDest: 312 Annotation: Subtype: Link Rect: 164, 237, 171, 249 ActionDest: 348 Annotation: Subtype: Link Rect: 192, 237, 199, 249 ActionDest: 348 Annotation: Subtype: Link Rect: 326, 167, 333, 179 ActionDest: 348 Annotation: Subtype: Link Rect: 254, 140, 261, 152 ActionDest: 348 Annotation: Subtype: Link Rect: 501, 127, 508, 138 ActionDest: 348 Page: Sequence: 9 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 396, 576, 403, 588 ActionDest: 416 Annotation: Subtype: Link Rect: 491, 232, 498, 244 ActionDest: 416 Annotation: Subtype: Link Rect: 160, 98, 172, 107 ActionDest: 564 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 99, 729, 106, 738 ActionDest: 496 Annotation: Subtype: Link Rect: 375, 729, 387, 738 ActionDest: 564 Annotation: Subtype: Link Rect: 503, 715, 515, 725 ActionDest: 564 Annotation: Subtype: Link Rect: 368, 580, 380, 590 ActionDest: 564 Annotation: Subtype: Link Rect: 196, 540, 208, 549 ActionDest: 564 Annotation: Subtype: Link Rect: 347, 499, 359, 508 ActionDest: 535 Annotation: Subtype: Link Rect: 360, 499, 372, 508 ActionDest: 535 Annotation: Subtype: Link Rect: 100, 472, 112, 481 ActionDest: 535 Annotation: Subtype: Link Rect: 152, 432, 164, 441 ActionDest: 564 Annotation: Subtype: Link Rect: 382, 405, 394, 414 ActionDest: 564 Annotation: Subtype: Link Rect: 401, 378, 413, 387 ActionDest: 564 Annotation: Subtype: Link Rect: 115, 351, 127, 360 ActionDest: 583 Annotation: Subtype: Link Rect: 503, 337, 515, 346 ActionDest: 583 Annotation: Subtype: Link Rect: 95, 310, 107, 319 ActionDest: 583 Annotation: Subtype: Link Rect: 258, 297, 270, 306 ActionDest: 583 Annotation: Subtype: Link Rect: 361, 297, 373, 306 ActionDest: 583 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 349, 491, 386, 501 Annotation: Subtype: Link Rect: 393, 491, 429, 501 Annotation: Subtype: Link Rect: 368, 464, 405, 475 Annotation: Subtype: Link Rect: 412, 464, 448, 475 Annotation: Subtype: Link Rect: 304, 425, 341, 436 Annotation: Subtype: Link Rect: 136, 375, 174, 385 Annotation: Subtype: Link Rect: 180, 375, 216, 385 Annotation: Subtype: Link Rect: 150, 323, 187, 333 Annotation: Subtype: Link Rect: 193, 323, 230, 333 Annotation: Subtype: Link Rect: 459, 309, 496, 320 Annotation: Subtype: Link Rect: 100, 258, 137, 268 Annotation: Subtype: Link Rect: 251, 219, 288, 230 Annotation: Subtype: Link Rect: 303, 180, 341, 191 Annotation: Subtype: Link Rect: 172, 142, 210, 152 Annotation: Subtype: Link Rect: 100, 103, 137, 113 Annotation: Subtype: Link Rect: 143, 103, 179, 113 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 288, 701, 326, 712 Annotation: Subtype: Link Rect: 332, 701, 368, 712 Annotation: Subtype: Link Rect: 475, 688, 513, 699 Annotation: Subtype: Link Rect: 141, 651, 178, 661 Annotation: Subtype: Link Rect: 184, 651, 220, 661 Annotation: Subtype: Link Rect: 136, 612, 174, 622 Annotation: Subtype: Link Rect: 180, 612, 216, 622 Annotation: Subtype: Link Rect: 154, 586, 192, 596 Annotation: Subtype: Link Rect: 198, 586, 234, 596 Annotation: Subtype: Link Rect: 100, 534, 137, 544 Annotation: Subtype: Link Rect: 352, 508, 390, 519 Annotation: Subtype: Link Rect: 424, 482, 462, 493 Annotation: Subtype: Link Rect: 100, 457, 137, 467 Annotation: Subtype: Link Rect: 100, 418, 137, 428 Annotation: Subtype: Link Rect: 143, 418, 179, 428 Annotation: Subtype: Link Rect: 478, 379, 516, 389 Annotation: Subtype: Link Rect: 100, 366, 136, 376 Annotation: Subtype: Link Rect: 186, 328, 223, 338 Annotation: Subtype: Link Rect: 437, 288, 474, 299 Annotation: Subtype: Link Rect: 480, 288, 516, 299 Annotation: Subtype: Link Rect: 127, 250, 165, 260 Annotation: Subtype: Link Rect: 421, 211, 458, 222 Annotation: Subtype: Link Rect: 360, 172, 397, 183 Annotation: Subtype: Link Rect: 236, 147, 273, 157 Annotation: Subtype: Link Rect: 479, 107, 516, 118 Annotation: Subtype: Link Rect: 284, 82, 321, 92 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 100, 702, 137, 712 Annotation: Subtype: Link Rect: 143, 702, 179, 712 Annotation: Subtype: Link Rect: 331, 663, 368, 673 Annotation: Subtype: Link Rect: 100, 625, 137, 635 Annotation: Subtype: Link Rect: 143, 625, 179, 635 Annotation: Subtype: Link Rect: 478, 585, 516, 596 Annotation: Subtype: Link Rect: 100, 573, 136, 583 Annotation: Subtype: Link Rect: 235, 534, 273, 544 Annotation: Subtype: Link Rect: 279, 534, 315, 544 Annotation: Subtype: Link Rect: 411, 469, 449, 480 Annotation: Subtype: Link Rect: 327, 430, 364, 441 Annotation: Subtype: Link Rect: 199, 405, 237, 415 Annotation: Subtype: Link Rect: 100, 354, 137, 364 Annotation: Subtype: Link Rect: 348, 314, 386, 325 Annotation: Subtype: Link Rect: 392, 314, 428, 325 Annotation: Subtype: Link Rect: 399, 288, 436, 299 Annotation: Subtype: Link Rect: 442, 288, 478, 299 Annotation: Subtype: Link Rect: 213, 250, 251, 260 Annotation: Subtype: Link Rect: 257, 250, 293, 260 Annotation: Subtype: Link Rect: 154, 212, 192, 222 Annotation: Subtype: Link Rect: 354, 185, 392, 196 Annotation: Subtype: Link Rect: 476, 146, 513, 157 Annotation: Subtype: Link Rect: 315, 120, 353, 131 Annotation: Subtype: Link Rect: 418, 82, 455, 92 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 462, 727, 500, 738 Annotation: Subtype: Link Rect: 172, 676, 210, 686 Annotation: Subtype: Link Rect: 248, 638, 285, 648 Annotation: Subtype: Link Rect: 292, 638, 328, 648 Annotation: Subtype: Link Rect: 136, 599, 174, 609 Annotation: Subtype: Link Rect: 180, 599, 216, 609 Annotation: Subtype: Link Rect: 163, 560, 201, 570 Annotation: Subtype: Link Rect: 207, 560, 243, 570 Annotation: Subtype: Link Rect: 76, 518, 166, 549 Annotation: Subtype: Link Rect: 234, 512, 407, 523 Checksum: ef480f6b Type: CRC32 Checksum: 386bad24b089a9762c010201ebb6c494 Type: MD5 Checksum: 17b4a6cef5e8c2b6b917b14a2881f2fcb787d39f Type: SHA-1