24961fc91ef7724132b5b7955789b3be CELLS_a2020n9p2546.pdf 5522dad8b5059d29b3da4c8115e110bf61feb35c CELLS_a2020n9p2546.pdf 08dce599b0ae539b2a528846fa3f2e9ae34dac13d11dc575ae69016e559c3c6d CELLS_a2020n9p2546.pdf Title: Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms Subject: This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner. Keywords: sperm; photobiology; mitochondrial function; mammalian; oligomycin A; FCCP Author: Olga Blanco-Prieto, Jaime Catalán, Lina Trujillo-Rojas, Alejandro Peña, Maria Montserrat Rivera del Álamo, Marc Llavanera, Sergi Bonet, Josep Maria Fernández-Novell, Marc Yeste and Joan E. 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ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-01-14 13:07:10 CET Size: 3296362 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 651 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Semen Samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Ethics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Sperm Motility Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Flow Cytometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Plasma Membrane Integrity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Acrosome Integrity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Mitochondrial Membrane Potential Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Intracellular Levels of Superoxide and Peroxide Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Intracellular Calcium Levels Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Determination of JC1-Staining Witch Laser Confocal Laser Microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Determination of Intracellular ATP Levels Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Determination of O2 Consumption Rate Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Determination of Cytochrome C Oxidase Activity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Children: Item: Title: Effects of Red-Light Stimulation on Plasma Membrane and Acrosome Integrity in the Presence or Absence of Either Oligomycin A or FCCP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Effects of Red-Light Stimulation on Sperm Motility in the Presence or Absence of Either Oligomycin A or FCCP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Effects of Red-Light Stimulation on Mitochondrial Membrane Potential in the Presence or Absence of Either Oligomycin A or FCCP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Effects of Red-Light Stimulation on Superoxide and Peroxide Levels in the Presence or Absence of Either Oligomycin A or FCCP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Effects of Red-Light Stimulation on Intracellular Calcium Levels in the Presence or Absence of Either Oligomycin A or FCCP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Effects of Red-Light Stimulation on Intracellular Levels of ATP and Oxygen Consumption in the Presence or Absence of Either Oligomycin A or FCCP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Effects of Red-Light Stimulation on Cytochrome C Oxidase (CCO) Activity in the Presence or Absence of Either Oligomycin A or FCCP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Info: Title: Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms Author: Olga Blanco-Prieto, Jaime Catalán, Lina Trujillo-Rojas, Alejandro Peña, Maria Montserrat Rivera del Álamo, Marc Llavanera, Sergi Bonet, Josep Maria Fernández-Novell, Marc Yeste and Joan E. Rodríguez-Gil Subject: This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner. 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Annotation: Subtype: Link Rect: 76, 281, 166, 312 Annotation: Subtype: Link Rect: 234, 275, 407, 286 Checksum: 88a871ab Type: CRC32 Checksum: 24961fc91ef7724132b5b7955789b3be Type: MD5 Checksum: 5522dad8b5059d29b3da4c8115e110bf61feb35c Type: SHA-1