7da4c72b03687b91ee508ef4ae71a276 252568.pdf d114ca74a1b8cc3ba740ab9d4c1ee171f25075ac 252568.pdf 39679ff019e969e0de77a6344ede5a10ae1f7977c035460c7ddc77017f8b5405 252568.pdf Title: Unravelling the consequences of the bacteriophages in human samples Subject: Scientific Reports, doi:10.1038/s41598-020-63432-7 Keywords: Author: Pedro Blanco-Picazo Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Tue Apr 14 09:27:47 2020 CEST ModDate: Wed Apr 15 14:57:38 2020 CEST Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 1932540 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XPFOEY+Corbel-Bold CID TrueType Identity-H yes yes yes 202 0 BRXSIQ+ArialUnicodeMS CID TrueType Identity-H yes yes yes 410 0 EuclidSymbol-Bold Type 1C Custom yes no yes 411 0 YOTHVV+Corbel-Bold2 Type 1C WinAnsi yes yes yes 50 0 MinionPro-Regular Type 1C Custom yes no yes 49 0 NPYJQC+Corbel2 Type 1C WinAnsi yes yes yes 53 0 PFKXWO+Corbel CID TrueType Identity-H yes yes yes 54 0 MyriadPro-It Type 1C WinAnsi yes no yes 55 0 MinionPro-Bold Type 1C Custom yes no yes 48 0 PBNSWO+STIXGeneral-Regular Type 1C Custom yes yes yes 51 0 MinionPro-It Type 1C Custom yes no yes 52 0 EuclidSymbol Type 1C Custom yes no yes 68 0 STIXGeneral-Bold Type 1C Custom yes no yes 69 0 MinionPro-BoldIt Type 1C WinAnsi yes no yes 139 0 UEBYJE+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 254 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-05-19 02:48:47 CEST RepresentationInformation: 252568.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-05-12 15:57:59 CEST Size: 1932540 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 1. Human samples in which bacteriophages were detected. CSF: Cerebrospinal fluid. *Only detected with E. coli WG5, with B. fragilis, P. aeruginosa and E. faecalis were not observed.:56' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 1. Human samples in which bacteriophages were detected. CSF: Cerebrospinal fluid. *Only detected with E. coli WG5, with B. fragilis, P. aeruginosa and E. faecalis were not observed.:58' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 1. Electron micrographs of phages from ascetic fluid, blood, serum, cerebrospinal fluid (CSF) and urine. (M) Myoviridae, (S) Siphoviridae, (P) Podoviridae, (I) Inoviridae and (NT) structures compatible with phage capsids b:60' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 1. Human samples in which bacteriophages were detected. CSF: Cerebrospinal fluid. *Only detected with E. coli WG5, with B. fragilis, P. aeruginosa and E. faecalis were not observed.:61' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 18. Fernández-Orth, D. et al. Faecal phageome of healthy individuals: presence of antibiotic resistance genes and variations caused by ciprofloxacin treatment. J. Antimicrob. Chemother 74, 854–864 (2019). :62' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 2. Viromes of the human samples and bacterial genes detected. *Number of different phages detected in each virome that show homology with sequences in the databases of phages infecting the bacterial host described:63' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 2. Viromes of the human samples and bacterial genes detected. *Number of different phages detected in each virome that show homology with sequences in the databases of phages infecting the bacterial host describe:64' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 2. Identification of the 16S rRNA gene sequences found in the virome of a pool of ascitic fluids, and four pools of urine samples. On the left side of the chart: 16S rRNA gene sequences of diverse bacterial genera identified in the virom:65' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 3. Bacterial genes detected in the virome of the samples. P: Plasmid, C: Chromosome, PH: Phage genome *Number of different 16S rRNA genes or different ARGs that were detected in each virome as verified by Kraken.:68' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 2. Identification of the 16S rRNA gene sequences found in the virome of a pool of ascitic fluids, and four pools of urine samples. On the left side of the chart: 16S rRNA gene sequences of diverse bacterial genera identified in the viro:69' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 2. Identification of the 16S rRNA gene sequences found in the virome of a pool of ascitic fluids, and four pools of urine samples. On the left side of the chart: 16S rRNA gene sequences of diverse bacterial genera identified in the vir:70' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 3. Bacterial genes detected in the virome of the samples. P: Plasmid, C: Chromosome, PH: Phage genome *Number of different 16S rRNA genes or different ARGs that were detected in each virome as verified by Kraken.:71' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 3. Phage reduction (log10 gene copy [GC]) of the Stx phage after filtration through 0.45 μm low-protein-binding polyvinylidene fluoride membranes (F) versus non-filtered samples (NF). The results correspond to three independent experim:72' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 3. Phage reduction (log10 gene copy [GC]) of the Stx phage after filtration through 0.45 μm low-protein-binding polyvinylidene fluoride membranes (F) versus non-filtered samples (NF). The results correspond to three independent experi:73' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 4. Values of phage (PFU/mL) and bacteria (CFU/mL) in the samples after the application of the filtration method (F) in comparison with the non-filtered controls (NF). In brackets, standard deviation.:74' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 4. Phages can bias the outcome of enrichment cultures. (A) Reduction (log10 PFU/mL) of phages infecting E. coli and P. aeruginosa in urine, AF and serum samples after the filtration procedure. (B) Recovery of culturable E. coli:75' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 4. Values of phage (PFU/mL) and bacteria (CFU/mL) in the samples after the application of the filtration method (F) in comparison with the non-filtered controls (NF). In brackets, standard deviation.:76' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 4. Phages can bias the outcome of enrichment cultures. (A) Reduction (log10 PFU/mL) of phages infecting E. coli and P. aeruginosa in urine, AF and serum samples after the filtration procedure. (B) Recovery of culturable E. col:77' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 4. Values of phage (PFU/mL) and bacteria (CFU/mL) in the samples after the application of the filtration method (F) in comparison with the non-filtered controls (NF). In brackets, standard deviation.:78' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 4. Phages can bias the outcome of enrichment cultures. (A) Reduction (log10 PFU/mL) of phages infecting E. coli and P. aeruginosa in urine, AF and serum samples after the filtration procedure. (B) Recovery of culturable E. co:79' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 15. Brown-Jaque, M., Muniesa, M. & Navarro, F. Bacteriophages in clinical samples can interfere with microbiological diagnostic tools. Sci. Rep 6, 33000 (2016). :80' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 12. Chen, J. et al. Genome hypermobility by lateral transduction. Science (80-.) 362, 207–212 (2018). :99' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 7. Thierauf, A., Perez, G. & Maloy, A. S. Generalized transduction. Methods Mol. Biol 501, 267–86 (2009). :101' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 37. Navarro, F. & Muniesa, M. Phages in the human body. Front. Microbiol 8, 566 (2017). :102' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 18. Fernández-Orth, D. et al. Faecal phageome of healthy individuals: presence of antibiotic resistance genes and variations caused by ciprofloxacin treatment. J. Antimicrob. Chemother 74, 854–864 (2019). :103' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd:Figure 2. Identification of the 16S rRNA gene sequences found in the virome of a pool of ascitic fluids, and four pools of urine samples. On the left side of the chart: 16S rRNA gene sequences of diverse bacterial genera identified in the vi:105' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 13. Brown-Jaque, M. et al. Antibiotic resistance genes in phage particles isolated from human feces and induced from clinical bacterial isolates. Int. J. Antimicrob. Agents 51, 434–442 (2018). :108' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: Table 2. Viromes of the human samples and bacterial genes detected. *Number of different phages detected in each virome that show homology with sequences in the databases of phages infecting the bacterial host describ:116' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 1. Adams, M. H. In Bacteriophages 592 (1959). :130' cannot be found Offset: 1495676 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_63432.indd: 52. Imamovic, L., Serra-Moreno, R., Jofre, J. & Muniesa, M. Quantification of Shiga toxin 2-encoding bacteriophages, by real-time PCR and correlation with phage infectivity. J. Appl. Microbiol 108, 1105–1114 (2010). :135' cannot be found Offset: 1495676 MIMEtype: application/pdf PDFMetadata: Objects: 506 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Unravelling the consequences of the bacteriophages in human samples Children: Item: Title: Results Children: Item: Title: Detection of phages in human samples. Item: Title: Virome analysis. Item: Title: Bacterial genes in the virome. Item: Title: Interference of phages with the molecular detection of bacteria. Item: Title: Bacterial strain recovery. Item: Title: Discussion Item: Title: Methods Children: Item: Title: Samples. Item: Title: Detection of phages in the samples. Item: Title: Transmission electron microscopy (TEM) studies. Item: Title: Virome analysis. Item: Title: Removal of phages from the sample. Item: Title: qPCR procedures. Item: Title: Statistical analysis. Item: Title: Acknowledgements Item: Title: Figure 1 Electron micrographs of phages from ascetic fluid, blood, serum, cerebrospinal fluid (CSF) and urine. Item: Title: Figure 2 Identification of the 16S rRNA gene sequences found in the virome of a pool of ascitic fluids, and four pools of urine samples. Item: Title: Figure 3 Phage reduction (log10 gene copy [GC]) of the Stx phage after filtration through 0. Item: Title: Figure 4 Phages can bias the outcome of enrichment cultures. Item: Title: Table 1 Human samples in which bacteriophages were detected. Item: Title: Table 2 Viromes of the human samples and bacterial genes detected. Item: Title: Table 3 Bacterial genes detected in the virome of the samples. Item: Title: Table 4 Values of phage (PFU/mL) and bacteria (CFU/mL) in the samples after the application of the filtration method (F) in comparison with the non-filtered controls (NF). 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Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: XPFOEY+Corbel-Bold Flags: Symbolic FontBBox: -556, -239, 1155, 952 FontFile2: true Font: BaseFont: BRXSIQ+ArialUnicodeMS CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: BRXSIQ+ArialUnicodeMS Flags: Symbolic FontBBox: -1011, -330, 2260, 1078 FontFile2: true XMP: application/pdf doi:10.1038/s41598-020-63432-7 Springer US Scientific Reports, doi:10.1038/s41598-020-63432-7 Unravelling the consequences of the bacteriophages in human samples Pedro Blanco-Picazo Dietmar Fernamp#x000E1;ndez-Orth Maryury Brown-Jaque Elisenda Miramp#x000F3; Paula Espinal Lorena Rodramp#x000ED;guez-Rubio Maite Muniesa Ferran Navarro 10.1038/s41598-020-63432-7 http://dx.doi.org/10.1038/s41598-020-63432-7 journal Scientific Reports © 2020, The Author(s) 2045-2322 10.1038/s41598-020-63432-7 Springer 2020-04-15T14:57:38+02:00 2020-04-14T12:57:47+05:30 2020-04-15T14:57:38+02:00 True iText® 5.3.5 ©2000-2012 1T3XT 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Permits publishers to include a second ISSN, identifying an electronic version of the issue in which the resource occurs (therefore e(lectronic)Issn. If used, prism:eIssn MUST contain the ISSN of the electronic version. See prism:issn. issn Text external Title of the magazine, or other publication, in which a resource was/will be published. Typically this will be used to provide the name of the magazine an article appeared in as metadata for the article, along with information such as the article title, the publisher, volume, number, and cover date. Note: Publication name can be used to differentiate between a print magazine and the online version if the names are different such as “magazine” and “magazine.com.” publicationName Text external This element provides the url for an article or unit of content. The attribute platform is optionally allowed for situations in which multiple URLs must be specified. PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. 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