938c7c49e2e397cbb0ad1a2320b422ca medicina-56-00408.pdf 8f76ad683c33a1fe7a2d939b6fa05218b1faecb8 medicina-56-00408.pdf cc807c689384b732fae99fee29dd8ef96b0660484d87c97c7048b7da78cae130 medicina-56-00408.pdf Title: UHPLC-HRMS and GC-MS Screening of a Selection of Synthetic Cannabinoids and Metabolites in Urine of Consumers Subject: Background and Objectives: The use of synthetic cannabinoids has increased around the world. As a result, the implementation of accurate analysis in human biological matrices is relevant and fundamental. Two different analytical technologies, ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and high-sensitivity gas chromatography-mass spectrometry (GC-MS) were used for the determination of three synthetic cannabinoids JWH-122, JWH 210, UR-144 and their metabolites in urine of consumers. Materials and Methods: Sample preparation included an initial hydrolysis with -glucuronidase and liquid-liquid extraction. The UHPLC-HRMS method included a Kinetex 2.6 u Biphenyl 100A (100 2.1 mm, 2.6 m) (Phenomenex, Italy) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode was used (mass range 100–1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m 250 m i.d, film thickness 0.25 m; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40–550 m/z). Results: Both methods have been successfully used for screening of parent synthetic cannabinoids and their metabolites in urine samples of consumers. Conclusions: The screening method applied JWH-122, JWH-210, UR-144 and their metabolites in urine of consumers can be applied to other compounds of the JWH family. Keywords: synthetic cannabinoids; urine; liquid chromatography; high-resolution mass spectrometry; gas chromatography-mass spectrometry Author: Manuela Pellegrini, Emilia Marchei, Esther Papaseit, Magí Farré and Simona Zaami Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Aug 13 13:45:22 2020 CEST ModDate: Thu Mar 18 08:39:34 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1128499 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 LNZVRW+Pxsy Type 1 Builtin yes yes no 15 0 YBCNYQ+VnURWPalladioL Type 1 Custom yes yes no 19 0 YRCRBO+URWPalladioL-Ital Type 1 Custom yes yes no 23 0 EPDZBO+URWPalladioL-Bold Type 1 Custom yes yes no 28 0 UFAPBI+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 MHAHCD+Rpxr Type 1 Builtin yes yes no 36 0 CAMXTK+EURM10 Type 1 Builtin yes yes no 40 0 QTLELQ+Pxbsy Type 1 Builtin yes yes no 44 0 HVRRJG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 52 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 74 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 78 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 82 0 BCDEEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 103 0 BCDFEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 107 0 BCDGEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 111 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 115 0 TimesNewRomanPSMT TrueType WinAnsi no no no 122 0 BCDEEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 135 0 BCDFEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 139 0 BCDGEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 143 0 TimesNewRomanPSMT TrueType WinAnsi no no no 147 0 BCDJEE+Calibri TrueType WinAnsi yes yes no 150 0 MFIGJL+Rpxb Type 1 Builtin yes yes no 209 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 219 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-26 02:34:45 CEST RepresentationInformation: medicina-56-00408.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-07-25 16:50:00 CEST Size: 1128499 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 306 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Methodology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Sample Preparation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Gas Chromatography-Mass Spectrometry (GC-MS) Instrumentation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Ultra-High-Performance Liquid Chromatography-High-Resolution Accurate Masses Spectrometry (UHPLC-HRMS) Instrumentation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Short Methods Validation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Info: Title: UHPLC-HRMS and GC-MS Screening of a Selection of Synthetic Cannabinoids and Metabolites in Urine of Consumers Author: Manuela Pellegrini, Emilia Marchei, Esther Papaseit, Magí Farré and Simona Zaami Subject: Background and Objectives: The use of synthetic cannabinoids has increased around the world. As a result, the implementation of accurate analysis in human biological matrices is relevant and fundamental. Two different analytical technologies, ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and high-sensitivity gas chromatography-mass spectrometry (GC-MS) were used for the determination of three synthetic cannabinoids JWH-122, JWH 210, UR-144 and their metabolites in urine of consumers. Materials and Methods: Sample preparation included an initial hydrolysis with -glucuronidase and liquid-liquid extraction. The UHPLC-HRMS method included a Kinetex 2.6 u Biphenyl 100A (100 2.1 mm, 2.6 m) (Phenomenex, Italy) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode was used (mass range 100 1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m 250 m i.d, film thickness 0.25 m; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40 550 m/z). Results: Both methods have been successfully used for screening of parent synthetic cannabinoids and their metabolites in urine samples of consumers. Conclusions: The screening method applied JWH-122, JWH-210, UR-144 and their metabolites in urine of consumers can be applied to other compounds of the JWH family. 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