375067d5be1845a676af97cd0a84aecf 283905.pdf bdccee3abddd70ed418f52e93ee066983df713f6 283905.pdf 3f253036388ce876ffc0401d4926f5351f2118521479df7b5bc5a07fdd9170db 283905.pdf Title: Usefulness of Two Independent DNA and RNA Tissue-Based Multiplex Assays for the Routine Care of Advanced NSCLC Patients Subject: Personalized medicine is nowadays a paradigm in lung cancer management, offering important benefits to patients. This study aimed to test the feasibility and utility of embedding two multiplexed genomic platforms as the routine workup of advanced non-squamous non-small cell lung cancer (NSCLC) patients. Two parallel multiplexed approaches were performed based on DNA sequencing and direct digital detection of RNA with nCounter® technology to evaluate gene mutations and fusions. The results were used to guide genotype-directed therapies and patient outcomes were collected. A total of 224 advanced non-squamous NSCLC patients were prospectively included in the study. Overall, 85% of samples were successfully characterized at DNA and RNA levels and oncogenic drivers were found in 68% of patients, with KRAS, EGFR, METex14, BRAF, and ALK being the most frequent (31%, 19%, 5%, 4%, and 4%, respectively). Among all patients with complete genotyping results and follow-up data (n = 156), the median overall survival (OS) was 1.90 years (confidence interval (CI) 95% 1.69–2.10) for individuals harbouring an actionable driver treated with a matched therapy, compared with 0.59 years (CI 95% 0.39–0.79) in those not eligible for any targeted therapy and 0.61 years (CI 95% 0.12–1.10) in patients with no drivers identified (p < 0.001). Integrating DNA and RNA multiplexing technologies into the routine molecular testing of advanced NSCLC patients is feasible and useful and highlights the necessity of widespread integrating comprehensive molecular diagnosis into lung cancer care. Keywords: advanced non-small cell lung cancer; molecular diagnostics; oncogenic drivers; mutations; targeted therapies Author: Elba Marin, Cristina Teixido, Elena Carmona-Rocha, Roxana Reyes, Ainara Arcocha, Nuria Viñolas, MªCarmen Rodríguez-Mues, Carlos Cabrera, Marcelo Sánchez, Ivan Vollmer, Sergi Castillo, Silvia Muñoz, Ivana G. Sullivan, Adela Rodriguez, Mireia Garcia, Silvia Alos, Pedro Jares, Antonio Martinez, Aleix Prat, Miguel Ángel Molina-Vila and Noemi Reguart Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Apr 30 11:41:41 2020 CEST ModDate: Thu Apr 30 11:41:41 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1845994 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EJIGOJ+URWPalladioL-Ital Type 1 Custom yes yes no 76 0 WGLYXJ+URWPalladioL-Bold Type 1 Custom yes yes no 77 0 YXDTOY+VnURWPalladioL-Bold Type 1 Custom yes yes no 78 0 VVTNLV+URWPalladioL-Roma Type 1 Custom yes yes no 79 0 VPJRUR+VnURWPalladioL Type 1 Custom yes yes no 80 0 SYXPPI+Rpxr Type 1 Builtin yes yes no 81 0 TGMEOR+Rpcxr Type 1 Builtin yes yes no 82 0 MPUDFL+PazoMath Type 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Annotation: Subtype: Link Rect: 315, 456, 353, 467 Annotation: Subtype: Link Rect: 76, 414, 166, 445 Annotation: Subtype: Link Rect: 234, 408, 407, 419 Checksum: e25167ca Type: CRC32 Checksum: 375067d5be1845a676af97cd0a84aecf Type: MD5 Checksum: bdccee3abddd70ed418f52e93ee066983df713f6 Type: SHA-1