7bbdc92ca722181fd5028f98a2caf2fe pmc_32751800.pdf 8cc3f46c163efe9ea8e73d16f612345658819382 pmc_32751800.pdf d2f9d7023ee8b5db394b2b977e4221a245d1bdafaa60b439bf23934df6d4f545 pmc_32751800.pdf Title: Microbial Signature in Adipose Tissue of Crohn’s Disease Patients Subject: Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity. Keywords: tissue microbiota; inflammatory bowel disease; 16S sequencing; creeping fat; PICRUSt analysis; Escherichia coli; Fusobacterium; lipopolysaccharide biosynthesis Author: Carolina Serena, Maribel Queipo-Ortuño, Monica Millan, Lidia Sanchez-Alcoholado, Aleidis Caro, Beatriz Espina, Margarita Menacho, Michelle Bautista, Diandra Monfort-Ferré, Margarida Terrón-Puig, Catalina Núñez-Roa, Elsa Maymó-Masip, M. Mar Rodriguez, Francisco J. Tinahones, Eloy Espin, Marc Martí, Sonia Fernández-Veledo and Joan Vendrell Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Jul 31 07:47:25 2020 CEST ModDate: Fri Jul 31 07:47:25 2020 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1904124 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QHPAVS+URWPalladioL-Ital Type 1 Custom yes yes no 83 0 HNDGHJ+URWPalladioL-Bold Type 1 Custom yes yes no 84 0 QTLELQ+Pxbsy Type 1 Builtin yes yes no 85 0 DGBDNV+VnURWPalladioL-Bold Type 1 Custom yes yes no 86 0 KQFMYE+URWPalladioL-Roma Type 1 Custom yes yes no 87 0 IQYYHS+VnURWPalladioL Type 1 Custom yes yes no 88 0 OFLXAT+Rpxr Type 1 Builtin yes yes no 89 0 WIYETI+Pxsy Type 1 Builtin yes yes no 90 0 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CID TrueType Identity-H yes yes yes 238 0 JMCPAB+Arial-BoldMT CID TrueType Identity-H yes yes yes 239 0 JMCODO+Cambria CID TrueType Identity-H yes yes yes 310 0 JMCPEC+Arial-BoldItalicMT TrueType WinAnsi yes yes no 311 0 JMCPFC+ArialMT CID TrueType Identity-H yes yes yes 312 0 JMDAAP+Calibri CID TrueType Identity-H yes yes yes 313 0 JMDJHG+Calibri-Bold CID TrueType Identity-H yes yes yes 314 0 JMDPHC+Calibri-BoldItalic CID TrueType Identity-H yes yes yes 315 0 JMCOFO+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 316 0 JMCOGP+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 317 0 JMCOHA+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 318 0 JMCOIA+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 319 0 JMCOJC+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 320 0 JMCOKC+SymbolMT CID TrueType Identity-H yes yes yes 321 0 JMCPAB+Arial-BoldMT CID TrueType Identity-H yes yes yes 322 0 JNLIIF+Rpxi Type 1 Builtin yes yes no 394 0 PJOCAD+PalatinoLinotype TrueType 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Item: Title: げぎぁ〠ぅへぴひちっぴどはの〠てひはね〠ごどびびふづび〠 Item: Title: ぐぃげ〠ぁねばぬどてどっちぴどはの〠ちのつ〠ぁのちぬべびどび〠はて〠〱〶こ〭ひげぎぁ〠こづぱふづのっづび〠 Item: Title: あどはどのてはひねちぴどっ〠ぁのちぬべびどび〠 Item: Title: こぴちぴどびぴどっちぬ〠ぁのちぬべびどび〠 Item: Title: ぅぴとどっび〠ぁばばひはぶちぬ〠ちのつ〠ぃはのびづのぴ〠ぴは〠ぐちひぴどっどばちぴづ〠 Item: Title: げづびふぬぴび〠 Children: Item: Title: きどっひはぢどはねづ〠こどでのちぴふひづ〠はて〠きづびづのぴづひどっ〠ぁつどばはびづ〠ごどびびふづ〠どの〠ぐちぴどづのぴび〠ぷどぴと〠ぃひはとの〙び〠いどびづちびづ〠 Item: Title: ごとづ〠きどっひはぢどはねづ〠こどでのちぴふひづ〠どの〠きづびづのぴづひどっ〠ぁつどばはびづ〠ごどびびふづ〠どの〠ぃひはとの〙び〠いどびづちびづ〠ぉび〠げづぬちぴづつ〠ぴは〠ぴとづ〠ぃぬどのどっちぬ〠こぴちぴふび〠 Item: Title: うふのっぴどはのちぬ〠いどててづひづのっづび〠どの〠ぴとづ〠ぇふぴ〠きどっひはぢどはぴち〠どの〠きづびづのぴづひどっ〠ぁつどばはびづ〠ごどびびふづ〠ぢづぴぷづづの〠ぁっぴどぶづ〠ちのつ〠ぉのちっぴどぶづ〠ぃひはとの〙び〠いどびづちびづ〠 Item: Title: いどびっふびびどはの〠 Item: Title: ぃはのっぬふびどはのび〠 Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QHPAVS+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: QHPAVS+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: 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