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2023-12-01 02:37:05 CET RepresentationInformation: pmc_33057023.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2020-11-02 03:37:06 CET Size: 4073244 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 986 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae Destination: title_link Children: Item: Title: Results Destination: A1 Children: Item: Title: Structure determination of P1 Destination: B1 Item: Title: Crystal structure of P40/P90N Destination: B2 Item: Title: Complexes of P40/P90N with sialylated oligosaccharides Destination: B3 Item: Title: Structural relationships of P1 and P40/P90 Destination: B4 Item: Title: Mapping of epitopes and genetic variability onto P1 and P40/P90 Destination: B5 Item: Title: Polyclonal antibodies against constructs of the C-terminal domain Destination: B6 Item: Title: Recognition of P1 and P40/P90 by sera from M. pneumoniae patients Destination: B7 Item: Title: Discussion Destination: A2 Item: Title: Methods Destination: A3 Children: Item: Title: Cloning, expression, and purification of P1 and P40/P90 constructs Destination: B8 Item: Title: Crystallization and X-ray data collection of P1 and P40/P90N Destination: B9 Item: Title: Determination of the P1 crystal structure Destination: B10 Item: Title: Determination of the P40/P90’structures Destination: B11 Item: Title: Single-particle cryo-EM Destination: B12 Item: Title: Single-particle cryo-EM image processing Destination: B13 Item: Title: SEC–nobreakMALS analysis Destination: B14 Item: Title: Antibody production and validation Destination: B15 Item: Title: Quantitative adhesion assay Destination: B16 Item: Title: Immunoassays Destination: B17 Item: Title: Ethical approval Destination: B18 Item: Title: Inductive coupled plasma–nobreakmass spectrometry (ICP–nobreakMS) Destination: B19 Item: Title: Surface plasmon resonance Destination: B20 Item: Title: Reporting summary Destination: A4 Item: Title: Data availability Destination: M1 Item: Title: References Destination: A5 Item: Title: Acknowledgements Destination: A6 Item: Title: Author contributions Destination: A7 Item: Title: Competing interests Destination: A8 Item: Title: Additional information Destination: M3 Info: Title: Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae Author: David Vizarraga Subject: Nature Communications, doi:10.1038/s41467-020-18777-y Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Thu Oct 08 05:03:51 CEST 2020 ModDate: Thu Oct 08 18:10:33 CEST 2020 ID: 0x456b4429fdc548f79bfe2bac4d8168b8, 0xb53ba924476f220301382dfb421e69a9 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: 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pathogen Mycoplasma pneumoniae David Vizarraga Akihiro Kawamoto U. Matsumoto Ramiro Illanes Rosa Pérez-Luque Jesús Martín Rocco Mazzolini Paula Bierge Oscar Q. Pich Mateu Espasa Isabel Sanfeliu Juliana Esperalba Miguel Fernández-Huerta Margot P. Scheffer Jaume Pinyol Achilleas S. Frangakis Maria Lluch-Senar Shigetarou Mori Keigo Shibayama Tsuyoshi Kenri Takayuki Kato Keiichi Namba Ignacio Fita Makoto Miyata David Aparicio Springer US Nature Communications, doi:10.1038/s41467-020-18777-y 10.1038/s41467-020-18777-y http://dx.doi.org/10.1038/s41467-020-18777-y journal Nature Communications © 2020, The Author(s) 2041-1723 10.1038/s41467-020-18777-y springer.com springerlink.com 2010-04-23 True 10.1038/s41467-020-18777-y springer.com springerlink.com Springer 2020-10-08T18:10:33+02:00 2020-10-08T08:03:51+05:30 2020-10-08T18:10:33+02:00 True iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) VoR uuid:bce0a9e1-7ab8-4c9f-b99a-4fa764409dbf uuid:b9a5703f-d7ca-43ee-a3b1-ad4e343aeb1a default 1 converted uuid:1b341fd6-a460-402d-9846-b77d47e4c1e6 converted to PDF/A-2b pdfToolbox 2020-10-08T08:04:43+05:30 2 B David Vizarraga http://orcid.org/0000-0002-4478-0155 Akihiro Kawamoto http://orcid.org/0000-0002-7380-0127 Ramiro Illanes http://orcid.org/0000-0002-1710-061X Oscar Q. 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PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. In lieu of using #other please reach out to the PRISM group at prism-wg@yahoogroups.com to request addition of your term to the Platform Controlled Vocabulary. url Text http://springernature.com/ns/xmpExtensions/2.0/ sn Springer Nature ORCID Schema external Author information: contains the name of each author and his/her ORCID (ORCiD: Open Researcher and Contributor ID). 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