77ae04821fa084902f4355c55156b705 pmc_33082423.pdf a78e8f5a2bdc71bdd600d498fdb90437dac60f97 pmc_33082423.pdf 0be341757653dd66ba2196fe73abec121cd93281717446342ede61087aae0130 pmc_33082423.pdf Title: In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos Subject: Scientific Reports, https://doi.org/10.1038/s41598-020-74961-6 Author: Meritxell Vendrell-Flotats Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Wed Oct 14 04:45:49 2020 CEST ModDate: Wed Oct 14 14:36:23 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 1750856 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OEVVMC+Wingdings-Regular Type 1C WinAnsi yes yes yes 591 0 MinionPro-Regular Type 1C Custom yes no yes 91 0 LZPUYB+Corbel-BoldItalic CID TrueType Identity-H yes yes yes 592 0 WCINWF+Corbel-Bold CID TrueType Identity-H yes yes yes 93 0 Corbel2 TrueType WinAnsi yes no yes 94 0 RCUOEP+Corbel CID TrueType Identity-H yes yes yes 95 0 MinionPro-It Type 1C Custom yes no yes 92 0 MinionPro-Bold Type 1C Custom yes no yes 110 0 KGZLKD+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 239 0 TimesNewRomanPSMT2 TrueType WinAnsi yes no yes 264 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-09-29 02:40:11 CEST RepresentationInformation: pmc_33082423.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-11-09 03:37:15 CET Size: 1750856 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 669 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos Children: Item: Title: Results Children: Item: Title: Effect of IL-6, IL-11 and LIF on miRNA expression in oocytes and cumulus cells. Item: Title: Effects of the addition of LIF during IVM on in vitro embryo developmental competence and levels of specific transcripts in blastocysts. Item: Title: Effects of LIF supplementation during IVM on levels of specific mRNA transcripts in oocytes and early embryo stages. Item: Title: Discussion Item: Title: Materials and methods Children: Item: Title: Chemicals and suppliers. Item: Title: Oocyte collection and in vitro maturation. Item: Title: In vitro fertilization and embryo culture. Item: Title: RNA extraction, reverse transcription and quantitative real-time PCR analysis for miRNA. Item: Title: RNA extraction, reverse transcription and quantitative real-time PCR analysis for mRNA. Item: Title: Differential staining of blastocysts. Item: Title: Experimental design. Children: Item: Title: Effect of IL-6, IL-11 and LIF on miRNA expression on oocytes and cumulus cells. Item: Title: Effects of the LIF addition during IVM on in vitro embryo developmental competence and levels of specific transcripts in blastocysts. Item: Title: Effects of the LIF addition during IVM on levels of specific transcripts in oocytes and early embryo stages. Item: Title: Statistical analysis. Item: Title: References Item: Title: Acknowledgements Info: Title: In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos Author: Meritxell Vendrell-Flotats Subject: Scientific Reports, https://doi.org/10.1038/s41598-020-74961-6 Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Wed Oct 14 05:15:49 CEST 2020 ModDate: Wed Oct 14 14:36:23 CEST 2020 ID: 0xf5c0575ff23130f600f7d02f26e2cf6f, 0xbc6d091ee9db9f522c8b340bc93ec729 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2032 ImageHeight: 1516 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2032 ImageHeight: 1649 ColorSpace: black is zero 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