3b44f4d5bf8c557dd06576ff1609e003 pmc_33105904.pdf 5e89bc35ecab1e737f8bf3f7b50830dc2e7adea7 pmc_33105904.pdf 9e681f6cc052fe4b1a0e01f93dc066096e373f20a78f84917d180061e5a82af9 pmc_33105904.pdf Title: Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans Subject: Few studies have analyzed the potential of biophysical parameters as markers of cardiac remodeling post-myocardial infarction (MI), particularly in human hearts. Fourier transform infrared spectroscopy (FTIR) illustrates the overall changes in proteins, nucleic acids and lipids in a single signature. The aim of this work was to define the FTIR and lipidomic pattern for human left ventricular remodeling post-MI. A total of nine explanted hearts from ischemic cardiomyopathy patients were collected. Samples from the right ventricle (RV), left ventricle (LV) and infarcted left ventricle (LV INF) were subjected to biophysical (FTIR and differential scanning calorimetry, DSC) and lipidomic (liquid chromatography–high-resolution mass spectrometry, LC–HRMS) studies. FTIR evidenced deep alterations in the myofibers, extracellular matrix proteins, and the hydric response of the LV INF compared to the RV or LV from the same subject. The lipid and esterified lipid FTIR bands were enhanced in LV INF, and both lipid indicators were tightly and positively correlated with remodeling markers such as collagen, lactate, polysaccharides, and glycogen in these samples. Lipidomic analysis revealed an increase in several species of sphingomyelin (SM), hexosylceramide (HexCer), and cholesteryl esters combined with a decrease in glycerophospholipids in the infarcted tissue. Our results validate FTIR indicators and several species of lipids as useful markers of left ventricular remodeling post-MI in humans. Keywords: biophysical markers; cardiac remodeling post-MI; lipidomics; Fourier transform infrared spectroscopy; heart failure Author: Valerie Samouillan, Ignacio Miguel Martinez de Lejarza Samper, Aleyda Benitez Amaro, David Vilades, Jany Dandurand, Josefina Casas, Esther Jorge, David de Gonzalo Calvo, Alberto Gallardo, Enrique Lerma, Jose Maria Guerra, Francesc Carreras, Ruben Leta and Vicenta Llorente Cortes Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Nov 5 09:23:46 2020 CET ModDate: Thu Nov 5 09:47:03 2020 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2905766 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TPGMUU+VnURWPalladioL Type 1 Custom yes yes no 10 0 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yes no 257 0 MDNMLH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 260 0 MDNNCG+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 263 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 285 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:18:03 CEST RepresentationInformation: pmc_33105904.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:43:31 CEST Size: 2905766 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 511 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Collection of Human Samples Destination: subsection.2.1 Item: Title: Tissue Homogenization and Preparation of the Samples for the Different Studies Destination: subsection.2.2 Item: Title: Immunohistochemical Analysis Destination: subsection.2.3 Item: Title: Vibrational Characterization Destination: subsection.2.4 Item: Title: Differential Scanning Calorimetry Destination: subsection.2.5 Item: Title: Lipidomic Analysis of the Heart Destination: subsection.2.6 Children: Item: Title: Phospholipids and Neutral Lipids Destination: subsubsection.2.6.1 Item: Title: Sphingolipids Destination: subsubsection.2.6.2 Item: Title: UPLC Coupled to HRMS Analysis Destination: subsubsection.2.6.3 Item: Title: Statistical Analysis Destination: subsection.2.7 Item: Title: Results Destination: section.3 Children: Item: Title: Identification of the Main FTIR Bands in the Human Heart Destination: subsection.3.1 Item: Title: Characterization of the Human Heart Lipidome Destination: subsection.3.2 Item: Title: Identification of the Alterations of the FTIR Bands Related to Myofibers and Structural Extracellular Matrix Proteins in Human LV INF compared to LV and RV Destination: subsection.3.3 Item: Title: Identification of the Alterations in the FTIR Bands Corresponding to DNA Response in Human LV INF Compared to LV and RV Destination: subsection.3.4 Item: Title: Study of the Hydric Response of Human RV, LV and LV INF Destination: subsection.3.5 Item: Title: Study of the Associations between FITR Variables in Human RV, LV and LV INF Destination: subsection.3.6 Item: Title: Identification of the Alterations in Sphingolipids, Glycerophospholipids and Neutral Lipids in Human LV INF Compared to LV and RV Destination: subsection.3.7 Item: Title: Study of the Association between FTIR and Lipidomic Variables in RV, LV and LV INF Destination: subsection.3.8 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans Author: Valerie Samouillan, Ignacio Miguel Martinez de Lejarza Samper, Aleyda Benitez Amaro, David Vilades, Jany Dandurand, Josefina Casas, Esther Jorge, David de Gonzalo Calvo, Alberto Gallardo, Enrique Lerma, Jose Maria Guerra, Francesc Carreras, Ruben Leta and Vicenta Llorente Cortes Subject: Few studies have analyzed the potential of biophysical parameters as markers of cardiac remodeling post-myocardial infarction (MI), particularly in human hearts. Fourier transform infrared spectroscopy (FTIR) illustrates the overall changes in proteins, nucleic acids and lipids in a single signature. The aim of this work was to define the FTIR and lipidomic pattern for human left ventricular remodeling post-MI. A total of nine explanted hearts from ischemic cardiomyopathy patients were collected. Samples from the right ventricle (RV), left ventricle (LV) and infarcted left ventricle (LV INF) were subjected to biophysical (FTIR and differential scanning calorimetry, DSC) and lipidomic (liquid chromatography high-resolution mass spectrometry, LC HRMS) studies. FTIR evidenced deep alterations in the myofibers, extracellular matrix proteins, and the hydric response of the LV INF compared to the RV or LV from the same subject. The lipid and esterified lipid FTIR bands were enhanced in LV INF, and both lipid indicators were tightly and positively correlated with remodeling markers such as collagen, lactate, polysaccharides, and glycogen in these samples. Lipidomic analysis revealed an increase in several species of sphingomyelin (SM), hexosylceramide (HexCer), and cholesteryl esters combined with a decrease in glycerophospholipids in the infarcted tissue. Our results validate FTIR indicators and several species of lipids as useful markers of left ventricular remodeling post-MI in humans. Keywords: biophysical markers; cardiac remodeling post-MI; lipidomics; Fourier transform infrared spectroscopy; heart failure Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Nov 05 09:23:46 CET 2020 ModDate: Thu Nov 05 09:47:03 CET 2020 ID: 0x82f071f134bcb4a0cb26ef0565056b5b, 0x82f071f134bcb4a0cb26ef0565056b5b Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: VUPETJ+Rpxb FontSubset: true FirstChar: 61 LastChar: 61 FontDescriptor: FontName: VUPETJ+Rpxb Flags: Symbolic FontBBox: -57, -266, 932, 729 FontFile: true Font: BaseFont: HHMDGK+Pxsy FontSubset: true FirstChar: 0 LastChar: 14 FontDescriptor: FontName: HHMDGK+Pxsy Flags: Symbolic FontBBox: -21, -994, 1292, 912 FontFile: true Font: BaseFont: VJHZXT+URWPalladioL-Bold FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: VJHZXT+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: 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