6173d30f90bd22df71e9119202faebd6 pmc_33162975.pdf b0165e2dde07678513fa3696bbdeb8a3676d9343 pmc_33162975.pdf e1164501d8af9c91351114b9028e37ce9333e55d649e0e5d541e7d3d06e84dfa pmc_33162975.pdf Title: Development of Pig Conventional Dendritic Cells From Bone Marrow Hematopoietic Cells in vitro Subject: In recent years, porcine dendritic cells (DCs) have been identified from pig tissues. Keywords: Flt3L, cDC1, cDC2, CD14C DC, pig Author: Yanli Li Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Thu Oct 8 08:33:46 2020 CEST ModDate: Thu Oct 8 11:55:38 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 3362648 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DPEFQQ+MinionPro-Regular Type 1 Custom yes yes no 24 0 EACXJH+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 25 0 GIIFFW+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 26 0 CCVXXJ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 31 0 FOOHQQ+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 32 0 SWPZFU+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 33 0 MLYTRF+MTSYN Type 1 Builtin yes yes no 34 0 CEOMIT+RBLMI Type 1 Builtin yes yes no 35 0 RWDYTP+MTGU Type 1 Builtin yes yes no 36 0 UZUSUO+MTSYB Type 1 Builtin yes yes no 90 0 HZQYWO+MinionPro-It Type 1 Custom yes yes no 94 0 THKLQT+MinionPro-Bold Type 1 Custom yes yes no 152 0 TAAFOS+MSAM7 Type 1 Builtin yes yes no 314 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-09-29 02:39:58 CEST RepresentationInformation: pmc_33162975.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-11-16 03:37:11 CET Size: 3362648 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 749 FreeObjects: 8 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Development of Pig Conventional Dendritic Cells From Bone Marrow Hematopoietic Cells in vitro Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Obtention of Bone Marrow Cells Item: Title: Generation of DCs From Flt3L-Dependent Bone Marrow Cultures Item: Title: Obtention of Alveolar Macrophages, Isolation of Peripheral Blood Mononuclear Cells and Monocytes, and Generation of Monocyte-Derived DCs Item: Title: Flow Cytometry Analysis Item: Title: RNA Extraction and Quantitative Real-Time PCR Item: Title: Dextran-FITC Uptake Item: Title: Phagocytosis of Staphylococcus aureus Item: Title: Mixed Lymphocyte Reaction Assay Item: Title: Response to TLR Agonist Stimulation: Maturation Markers and Cytokine Patterns Item: Title: Effect of SCF, GM-CSF, and IL-4 Addition to Flt3L-Based BMHC Cultures Item: Title: Statistical Analysis Item: Title: Results Children: Item: Title: Three Distinct Cell Populations Can Be Identified in Flt3L-Derived Bone Marrow Cell Cultures Item: Title: Development Process of Flt3L-Derived Bone Marrow Cell Cultures Item: Title: Expression of Conserved DC Markers Assessed by RT-qPCR Item: Title: Functional Analysis of Putative cDC1, cDC2, and CD14+ DCs Item: Title: Response to TLR Agonist Stimulation: Maturation Markers and Cytokine Patterns Item: Title: Addition of SCF, GM-CSF, or IL-4 Does Not Improve the Production of cDCs Item: Title: Discussion Item: Title: Data Availability Statement Item: Title: Ethics Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: Development of Pig Conventional Dendritic Cells From Bone Marrow Hematopoietic Cells in vitro Author: Yanli Li Subject: In recent years, porcine dendritic cells (DCs) have been identified from pig tissues. 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