3d0757b7feca826d950cb13b94aa6dd7 pmc_33255666.pdf d569896a18386a29e72b7882f92399bc67b0fff5 pmc_33255666.pdf 06fb342d7c1e5407080d2f29026b1545ee9c636b9f2afe51780ae1c6da1ff859 pmc_33255666.pdf Title: Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits Subject: The maternal environment modulates immune responses to facilitate embryo development and ensure pregnancy. Unraveling this modulation could improve the livestock breeding systems. Here it is hypothesized that the exposure of the female rabbit reproductive tract to semen, as well as to early embryos, modulates inflammation and angiogenesis among different tissue segments. qPCR analysis of the gene expression changes of the anti-inflammatory interleukin-10 (IL10) and transforming growth factor beta family (TGF1–3) and the angiogenesis mediator vascular endothelial growth factor (VEGF-A) were examined in response to mating or insemination with sperm-free seminal plasma (SP). Reproductive tract segment (cervix to infundibulum) samples were obtained in Experiment 1, 20 h after gonadotropin-releasing hormone (GnRH) stimulation (control), natural mating (NM) or vaginal infusion with sperm-free SP (SP-AI). Additionally, segmented samples were also obtained at 10, 24, 36, 68 or 72 h after GnRH-stimulation and natural mating (Experiment 2). The results of gene expression, analyzed by quantitative PCR, showed that NM effects were mainly localized in the uterine tissues, depicting clear temporal variation, while SP-AI effects were restricted to the oviduct. Changes in anti-inflammatory and angiogenesis mediators indicate an early response in the uterus and a late modulation in the oviduct either induced by semen or preimplantation embryos. This knowledge could be used in the implementation of physiological strategies in breeding systems to face the new challenges on rabbit productivity and sustainability. Keywords: gene expression; endometrium; oviduct; spermatozoa; seminal plasma; inflammation; angiogenesis; rabbit Author: Jaume Gardela, Amaia Jauregi-Miguel, Cristina A. Martinez, Heriberto Rodríguez-Martinez, Manel López-Béjar and Manuel Álvarez-Rodríguez Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Wed Nov 25 11:49:11 2020 CET ModDate: Wed Nov 25 11:49:11 2020 CET Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1051768 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OPKANF+URWPalladioL-Ital Type 1 Custom yes yes no 88 0 AAHINN+URWPalladioL-Bold Type 1 Custom yes yes no 89 0 QZKPTP+VnURWPalladioL-Bold Type 1 Custom yes yes no 90 0 FDOHGW+URWPalladioL-Roma Type 1 Custom yes yes no 91 0 JIAXHG+VnURWPalladioL Type 1 Custom yes yes no 92 0 IELMKZ+Rpxr Type 1 Builtin yes yes no 93 0 LWLIVD+EURM10 Type 1 Builtin yes yes no 94 0 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ぎちぴふひちぬ〠きちぴどので〠ちのつ〠こばづひね〭うひづづ〠こづねどのちぬ〠ぐぬちびねち〠ぉのてふびどはの〠いどててづひづのぴどちぬぬべ〠きはつふぬちぴづつ〠ぁのぴど〭ぉのてぬちねねちぴはひべ〠ぃべぴはにどのづび〠ちのつ〠ざぅぇう〭ぁ〠ぇづのづび〠 Item: Title: ごづねばはひちぬ〠ぇづのづ〠ぅへばひづびびどはの〠はて〠ぁのぴど〭ぉのてぬちねねちぴはひべ〠っべぴはにどのづび〠ちのつ〠ざぅぇう〭ぁ〠ちぴ〠〱〰〠と〠ふば〠ぴは〠〷〲〠と〠ぐはびぴ〭きちぴどので〠 Item: Title: こばちぴどちぬ〠ぇづのづ〠ぅへばひづびびどはの〠ごひどででづひづつ〠ぢべ〠ぎちぴふひちぬ〠きちぴどので〠ちのつ〠こばづひね〭うひづづ〠こづねどのちぬ〠ぐぬちびねち〠ぉのてふびどはの〠 Item: Title: こばちぴどちぬ〠ぇづのづ〠ぅへばひづびびどはの〠ちぴ〠〱〰〠と〠ふば〠ぴは〠〷〲〠と〠ぐはびぴ〭きちぴどので〠 Item: Title: いどびっふびびどはの〠 Item: Title: ぃはのっぬふびどはのび〠 Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: GBLQZB+Rpxi FontSubset: true FirstChar: 11 LastChar: 11 FontDescriptor: FontName: GBLQZB+Rpxi Flags: Symbolic FontBBox: -162, -280, 856, 733 FontFile: true Font: BaseFont: YNURIB+Rpxmi FontSubset: true FirstChar: 12 LastChar: 12 FontDescriptor: FontName: YNURIB+Rpxmi Flags: Symbolic FontBBox: -70, -289, 949, 802 FontFile: true Font: BaseFont: DFIRNB+Pxsy FontSubset: true FirstChar: 0 LastChar: 48 FontDescriptor: FontName: DFIRNB+Pxsy Flags: 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