9287d10310e45495d5e004f4c8a793e4 pmc_33255984.pdf 9587f356a2bcf16531b3af9399a1a6aabe3a20af pmc_33255984.pdf 08502516b8e1eb0bead889fd5c949715cf07f187cd36d92133ef14b9a17c607f pmc_33255984.pdf Title: Helpful Criteria When Implementing NGS Panels in Childhood Lymphoblastic Leukemia Subject: The development of Next-Generation Sequencing (NGS) has provided useful diagnostic, prognostic, and therapeutic strategies for individualized management of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) patients. Consequently, NGS is rapidly being established in clinical practice. However, the technology’s complexity, bioinformatics analysis, and the different available options difficult a broad consensus between different laboratories in its daily routine introduction. This collaborative study among Spanish centers was aimed to assess the feasibility, pros, and cons of our customized panel and other commercial alternatives of NGS-targeted approaches. The custom panel was tested in three different sequencing centers. We used the same samples to assess other commercial panels (OncomineTM Childhood Cancer Research Assay; Archer®FusionPlex® ALL, and Human Comprehensive Cancer Panel GeneRead Panel v2®). Overall, the panels showed a good performance in different centers and platforms, but each NGS approach presented some issues, as well as pros and cons. Moreover, a previous consensus on the analysis and reporting following international guidelines would be preferable to improve the concordance in results among centers. Our study shows the challenges posed by NGS methodology and the need to consider several aspects of the chosen NGS-targeted approach and reach a consensus before implementing it in daily practice. Keywords: next-generation sequencing; NGS-targeted panel; childhood acute lymphoblastic leukemia Author: Nerea Vega-Garcia, Rocío Benito, Elena Esperanza-Cebollada, Marta Llop, Cristina Robledo, Clara Vicente-Garcés, Javier Alonso, Eva Barragán, Guerau Fernández, Jesús M. Hernández-Sánchez, Marta Martín-Izquierdo, Joan Maynou, Alfredo Minguela, Adrián Montaño, Margarita Ortega, Montserrat Torrebadell, José Cervera, Joaquín Sánchez, Antonio Jiménez-Velasco, Susana Riesco, Jesús M. Hernández-Rivas, Álvaro Lassaletta, José María Fernández, Susana Rives, José Luis Dapena, Manuel Ramírez, Mireia Camós and on behalf of the Group of Leukemia of the Spanish Society of Pediatric Hematology and Oncology (SEHOP) Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Nov 26 10:40:29 2020 CET ModDate: Thu Nov 26 10:40:29 2020 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1624626 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TRDZIV+URWPalladioL-Ital Type 1 Custom yes yes no 141 0 FFZYQB+URWPalladioL-Bold Type 1 Custom yes yes no 142 0 WIRPUU+VnURWPalladioL-Bold Type 1 Custom yes yes no 143 0 CUAJJQ+Pxbsy Type 1 Builtin yes yes no 144 0 LYQIKS+URWPalladioL-Roma Type 1 Custom yes yes no 145 0 ARIQJE+VnURWPalladioL Type 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no no 264 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 300 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 301 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 302 0 Times New Roman TrueType WinAnsi no no no 303 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-07-12 02:36:04 CEST RepresentationInformation: pmc_33255984.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-12-14 03:46:45 CET Size: 1624626 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぅぴとどっちぬぜ〴ぜ〰ぉびびふづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰いづびどでのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こぴふつべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぎぇこ〭ごちひでづぴづつぜ〴ぜ〰ぐちのづぬびぜ〴ぜ〰ぉのっぬふつづつぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰こぴふつべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Children: Item: Title: ぅぴとどっちぬぜ〴ぜ〰ぉびびふづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰いづびどでのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こぴふつべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぎぇこ〭ごちひでづぴづつぜ〴ぜ〰ぐちのづぬびぜ〴ぜ〰ぉのっぬふつづつぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰こぴふつべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Item: Title: いづびどでのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こぴふつべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぎぇこ〭ごちひでづぴづつぜ〴ぜ〰ぐちのづぬびぜ〴ぜ〰ぉのっぬふつづつぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰こぴふつべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Item: Title: ぎぇこ〭ごちひでづぴづつぜ〴ぜ〰ぐちのづぬびぜ〴ぜ〰ぉのっぬふつづつぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰こぴふつべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Children: Item: Title: あぃぐ〭ぁががぜ〴ぜ〰ぃふびぴはねぜ〴ぜ〰ぐちのづぬぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸲ Item: Title: ぁひっとづひぜ㈵㙜〰ぜ〴ぜ〰うふびどはのぐぬづへぜ㈵㙜〰ぜ〴ぜ〰ぁががぜ〴ぜ〰かどぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸳ Item: Title: くのっはねどのづごきぜ〴ぜ〰ぃとどぬつとははつぜ〴ぜ〰ぃちのっづひぜ〴ぜ〰げづびづちひっとぜ〴ぜ〰ぁびびちべぜ〴ぜ〰ぜ〵ぜ〰くぃぃげぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸴ Item: Title: えふねちのぜ〴ぜ〰ぃはねばひづとづのびどぶづぜ〴ぜ〰ぃちのっづひぜ〴ぜ〰ぇづのづげづちつぜ〴ぜ〰ぐちのづぬぜ〴ぜ〰ぶ〲ぜ㈵㙜〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰こづぱふづのっどのでぜ〴ぜ〰ちのつぜ〴ぜ〰ざちひどちのぴぜ〴ぜ〰いちぴちぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こづぱふづのっどのでぜ〴ぜ〰ちのつぜ〴ぜ〰ざちひどちのぴぜ〴ぜ〰いちぴちぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰いどびびづっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぎぇこぜ〴ぜ〰ぐちのづぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸱⸱ Children: Item: Title: いどびびづっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぎぇこぜ〴ぜ〰ぐちのづぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸱⸱ Children: Item: Title: あぃぐ〭ぁががぜ〴ぜ〰ぃふびぴはねぜ〴ぜ〰ぐちのづぬぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸱⸲ Item: Title: ぁひっとづひぜ㈵㙜〰ぜ〴ぜ〰うふびどはのぐぬづへぜ㈵㙜〰ぜ〴ぜ〰ぁががぜ〴ぜ〰かどぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸱⸳ Item: Title: くのっはねどのづごきぜ〴ぜ〰ぃとどぬつとははつぜ〴ぜ〰ぃちのっづひぜ〴ぜ〰げづびづちひっとぜ〴ぜ〰ぁびびちべぜ〴ぜ〰ぜ〵ぜ〰くぃぃげぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸱⸴ Item: Title: えふねちのぜ〴ぜ〰ぃはねばひづとづのびどぶづぜ〴ぜ〰ぃちのっづひぜ〴ぜ〰ぇづのづげづちつぜ〴ぜ〰ぐちのづぬぜ〴ぜ〰ぶ〲ぜ㈵㙜〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぐちのづぬぜ〴ぜ〰いどびびづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Item: Title: ぐちのづぬぜ〴ぜ〰いどびびづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸱ Children: Item: Title: こぎざぜ〴ぜ〰いづぴづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸲ Item: Title: ぃぎざぜ〴ぜ〰いづぴづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸳⸲⸳ Item: Title: げぎぁぜ〴ぜ〰うふびどはのぜ〴ぜ〰いづぴづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐちのづぬぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰ぉねばぬづねづのぴどのでぜ〴ぜ〰ぎぇこぜ〴ぜ〰どのぜ〴ぜ〰ぐづつどちぴひどっぜ〴ぜ〰あぃぐ〭ぁががぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸴⸲⸱ Children: Item: Title: ぐちのづぬぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰ぉねばぬづねづのぴどのでぜ〴ぜ〰ぎぇこぜ〴ぜ〰どのぜ〴ぜ〰ぐづつどちぴひどっぜ〴ぜ〰あぃぐ〭ぁががぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸴⸲⸱ Item: Title: ぉねばぬづねづのぴどのでぜ〴ぜ〰ぎぇこぜ〴ぜ〰どのぜ〴ぜ〰ぐづつどちぴひどっぜ〴ぜ〰あぃぐ〭ぁががぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸴⸲⸱ Children: Item: Title: ごとづぜ〴ぜ〰ぐちのづぬぜ〴ぜ〰ぃとはどっづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸴⸲⸲ Item: Title: ごづっとのどっちぬぜ〴ぜ〰ぃとちぬぬづのでづびぜ〴ぜ〰はてぜ〴ぜ〰ぅちっとぜ〴ぜ〰ぁのちぬべぺづつぜ〴ぜ〰ぐちのづぬぜ〴ぜ〰てはひぜ〴ぜ〰あぃぐ〭ぁががぜ〴ぜ〰いどちでのはびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸴⸲⸳ Item: Title: いちぴちぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰てはひぜ〴ぜ〰ざちひどちのぴぜ〴ぜ〰いづぴづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸴⸲⸴ Item: Title: ざちひどちのぴぜ〴ぜ〰ぐひどはひどぴどぺちぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰げづばはひぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのび〺ぜ〴ぜ〰がづびびはのびぜ〴ぜ〰がづちひのづつぜ〴ぜ〰ちのつぜ〴ぜ〰ぴとづぜ〴ぜ〰うふぴふひづぜ〴ぜ〰ぁとづちつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: CUAJJQ+Pxbsy 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