780e6b8d56eec059521f94fd0c9fce67 pmc_33260846.pdf c10935a12bfc328de8f91d7a702744a8f3393b7b pmc_33260846.pdf 993395e3441ef31d88d4c9965b1d2c4640aca8dacbc567d7db319d786d2f6881 pmc_33260846.pdf Title: Changes in Acute Phase Proteins in Bitches after Laparoscopic, Midline, and Flank Ovariectomy Using the Same Method for Hemostasis Subject: Acute phase proteins (APP) are biomarkers of systemic inflammation, which allow monitoring the evolution of diseases, the response to treatments, and post-operative complications. Ovariectomy (OVE) is frequently performed in veterinary medicine and can be a useful model to evaluate surgical trauma and inflammation in the bitch. The objective was to investigate and compare the acute phase response (APR) after applying three different OVE techniques by measuring serum levels of C-reactive protein (CRP), haptoglobin (Hp), albumin (Alb), and paraoxonase-1 (PON-1). Forty-five intact bitches were included in the study, being randomly distributed into three groups: laparoscopic OVE (L), midline OVE (M), and flank OVE (F). Serum CRP, Hp, Alb, and PON-1 were measured before surgery, 1, 24, 72, and 168 h post-intervention. CRP levels increased significantly 24 h post-surgery in the M and F groups, but no significant variation was observed in the L group at any time of the study period. Hp was significantly higher in group L than in group F 72 h post-surgery. Alb and PON-1 showed no statistical difference among groups or among sampling periods. CRP response suggests that the use of laparoscopic procedures produce lower inflammation compared to open conventional approaches when performing OVE in the bitch. Keywords: acute phase proteins; surgical trauma; ovariectomy; laparoscopy Author: Ayla Del Romero, Belén Cuervo, Pau Peláez, Laura Miguel, Marta Torres, Marc Yeste, Maria Montserrat Rivera del Alamo, Camila P. Rubio and Mónica Rubio Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Nov 27 07:44:38 2020 CET ModDate: Mon Nov 30 09:48:07 2020 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1986010 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZZTQUO+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 IEFSKB+VnURWPalladioL Type 1 Custom yes yes no 15 0 MIGDOU+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 PEMUQH+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 NKUBAD+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 IELMKZ+Rpxr Type 1 Builtin yes yes no 32 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 62 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 66 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 70 0 YICMPF+Rpcxr Type 1 Builtin yes yes no 83 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 87 0 DQGBIX+Pxsy Type 1 Builtin yes yes no 94 0 HOHHFN+PalatinoLinotype TrueType WinAnsi yes yes no 104 0 HOHHHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 107 0 HOHHJA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 110 0 HOHHFN+PalatinoLinotype TrueType WinAnsi yes yes no 118 0 HOHHHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 121 0 HOHHJA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 124 0 WYANLJ+Rpxi Type 1 Builtin yes yes no 132 0 HOHHFN+PalatinoLinotype TrueType WinAnsi yes yes no 139 0 HOHHHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 142 0 HOHHJA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 145 0 HOHHFN+PalatinoLinotype TrueType WinAnsi yes yes no 153 0 HOHHHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 156 0 HOHHJA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 159 0 HOHHFN+PalatinoLinotype TrueType WinAnsi yes yes no 170 0 HOHHHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 173 0 HOHHJA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 176 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:17:27 CEST RepresentationInformation: pmc_33260846.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:43:36 CEST Size: 1986010 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 476 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals Destination: subsection.2.1 Item: Title: Study Groups Destination: subsection.2.2 Item: Title: Anesthesia and Antibiotic Prophylaxis Destination: subsection.2.3 Item: Title: Surgical Procedures Destination: subsection.2.4 Item: Title: Blood Sampling Destination: subsection.2.5 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results Destination: section.3 Children: Item: Title: CRP and Hp Destination: subsection.3.1 Item: Title: Alb and PON-1 Destination: subsection.3.2 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Changes in Acute Phase Proteins in Bitches after Laparoscopic, Midline, and Flank Ovariectomy Using the Same Method for Hemostasis Author: Ayla Del Romero, Belén Cuervo, Pau Peláez, Laura Miguel, Marta Torres, Marc Yeste, Maria Montserrat Rivera del Alamo, Camila P. Rubio and Mónica Rubio Subject: Acute phase proteins (APP) are biomarkers of systemic inflammation, which allow monitoring the evolution of diseases, the response to treatments, and post-operative complications. Ovariectomy (OVE) is frequently performed in veterinary medicine and can be a useful model to evaluate surgical trauma and inflammation in the bitch. The objective was to investigate and compare the acute phase response (APR) after applying three different OVE techniques by measuring serum levels of C-reactive protein (CRP), haptoglobin (Hp), albumin (Alb), and paraoxonase-1 (PON-1). Forty-five intact bitches were included in the study, being randomly distributed into three groups: laparoscopic OVE (L), midline OVE (M), and flank OVE (F). Serum CRP, Hp, Alb, and PON-1 were measured before surgery, 1, 24, 72, and 168 h post-intervention. CRP levels increased significantly 24 h post-surgery in the M and F groups, but no significant variation was observed in the L group at any time of the study period. Hp was significantly higher in group L than in group F 72 h post-surgery. Alb and PON-1 showed no statistical difference among groups or among sampling periods. CRP response suggests that the use of laparoscopic procedures produce lower inflammation compared to open conventional approaches when performing OVE in the bitch. Keywords: acute phase proteins; surgical trauma; ovariectomy; laparoscopy Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Nov 27 07:44:38 CET 2020 ModDate: Mon Nov 30 09:48:07 CET 2020 ID: 0x8d764158eb6f28abfdc9da9badc399fe, 0x8d764158eb6f28abfdc9da9badc399fe Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: IELMKZ+Rpxr FontSubset: true FirstChar: 11 LastChar: 61 FontDescriptor: FontName: IELMKZ+Rpxr Flags: Symbolic FontBBox: -40, -283, 904, 728 FontFile: true Font: BaseFont: MIGDOU+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: MIGDOU+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: YICMPF+Rpcxr FontSubset: true FirstChar: 151 LastChar: 174 FontDescriptor: FontName: YICMPF+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: WYANLJ+Rpxi FontSubset: true FirstChar: 61 LastChar: 61 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