02d51eb2411a1dfe1ce187f940b65371 pmc_33261078.pdf ba51129e1e0dd2fa18b230bee8e3699174e61fb7 pmc_33261078.pdf 2749f2a74106902cf1636be2554bfb7f4322cfb036c7adf488cdc399fef6ad77 pmc_33261078.pdf Title: Hastening Time to Ejaculation in Donkey Jacks Treated with the PGF2 Analog, Cloprostenol Sodium Subject: Due to the long courtship needed to attain excitation and erection, donkey semen collection can take up to 90 min. ProstaglandinF2 (PGF2) has been reported to hasten the onset of erection and ejaculation in domesticated mammals, presumably by inducing smooth muscle contractions in the internal genitalia. However, while it has been anecdotally used in donkeys, it has yet to be critically evaluated. This study aimed to compare behavioral and semen parameters in Catalan, Balearic, Amiata, and Miranda jacks treated with the PGF2 analogue cloprostenol sodium immediately prior to exposure to an estrus jenny. Nineteen donkeys were assigned in a crossover design to receive cloprostenol sodium (125 g, i.m.; n = 53 collections) or saline (1 mL, i.m.; n = 53 collections). There were no differences for erection (52/53 vs. 52/53) or ejaculation (52/53 vs. 48/53) for collection attempts assigned to saline or cloprostenol sodium, respectively. Cloprostenol sodium significantly hastened treatment-to-erection and treatment-to-ejaculation times from 12.0 1.6 to 6.0 1.6 min and from 14.0 1.4 to 9.6 1.4 min, respectively. Significant effects of breed and age were observed in behavioral and parameters, but there were no effects of cloprostenol sodium administration on semen parameters. In conclusion, cloprostenol sodium administration immediately prior to semen collection hastened time to collect semen in donkeys with no detrimental effects on semen quality and can be used by practitioners to circumvent long delays in donkey semen collection. Keywords: donkey; semen collection; cloprostenol sodium; erection; ejaculation Author: Duccio Panzani, Miguel Quaresma, Diana Fanelli, Francesco Camillo, Rebecca Moroni, Alessandra Rota, Ana Martins-Bessa, Miguel Nóvoa, Jaime Catalán, Igor F. Canisso, Giuseppe Conte and Jordi Mirò Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.19 CreationDate: Thu Dec 3 11:03:16 2020 CET ModDate: Thu Dec 3 11:09:22 2020 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2257911 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZXDKIP+EURM10 Type 1 Builtin yes yes no 10 0 HDGIXW+VnURWPalladioL-Bold Type 1 Custom yes yes no 14 0 VVCFQA+VnURWPalladioL Type 1 Custom yes yes no 19 0 UXDAZF+URWPalladioL-Ital Type 1 Custom yes yes no 23 0 BJHHCW+URWPalladioL-Bold Type 1 Custom yes yes no 28 0 TTWDOU+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 OFLXAT+Rpxr Type 1 Builtin yes yes no 36 0 CZOINJ+EURB10 Type 1 Builtin yes yes no 40 0 CHOIGB+Pxsy Type 1 Builtin yes yes no 44 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 52 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 74 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 78 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 82 0 TGMEOR+Rpcxr Type 1 Builtin yes yes no 92 0 PHSWUJ+Rpxb Type 1 Builtin yes yes no 102 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 106 0 ITYRUV+VnURWPalladioL-Italic Type 1 Custom yes yes no 133 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-06-10 02:24:10 CEST RepresentationInformation: pmc_33261078.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-12-28 03:46:59 CET Size: 2257911 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 276 FreeObjects: 3 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Animals and Locations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Behavioral Assessment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Semen Assessment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Info: Title: Hastening Time to Ejaculation in Donkey Jacks Treated with the PGF2 Analog, Cloprostenol Sodium Author: Duccio Panzani, Miguel Quaresma, Diana Fanelli, Francesco Camillo, Rebecca Moroni, Alessandra Rota, Ana Martins-Bessa, Miguel Nóvoa, Jaime Catalán, Igor F. Canisso, Giuseppe Conte and Jordi Mirò Subject: Due to the long courtship needed to attain excitation and erection, donkey semen collection can take up to 90 min. ProstaglandinF2 (PGF2) has been reported to hasten the onset of erection and ejaculation in domesticated mammals, presumably by inducing smooth muscle contractions in the internal genitalia. However, while it has been anecdotally used in donkeys, it has yet to be critically evaluated. This study aimed to compare behavioral and semen parameters in Catalan, Balearic, Amiata, and Miranda jacks treated with the PGF2 analogue cloprostenol sodium immediately prior to exposure to an estrus jenny. Nineteen donkeys were assigned in a crossover design to receive cloprostenol sodium (125 g, i.m.; n = 53 collections) or saline (1 mL, i.m.; n = 53 collections). There were no differences for erection (52/53 vs. 52/53) or ejaculation (52/53 vs. 48/53) for collection attempts assigned to saline or cloprostenol sodium, respectively. Cloprostenol sodium significantly hastened treatment-to-erection and treatment-to-ejaculation times from 12.0 1.6 to 6.0 1.6 min and from 14.0 1.4 to 9.6 1.4 min, respectively. Significant effects of breed and age were observed in behavioral and parameters, but there were no effects of cloprostenol sodium administration on semen parameters. In conclusion, cloprostenol sodium administration immediately prior to semen collection hastened time to collect semen in donkeys with no detrimental effects on semen quality and can be used by practitioners to circumvent long delays in donkey semen collection. 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