471038038e48272495c081baa84ef946 pmc_33365309.pdf ccd55f0010d423d0613309b518de4c4228340a82 pmc_33365309.pdf d91c1d77297af1b02650ef7024428348ef760a96d0a1c0643b479bf371e19663 pmc_33365309.pdf Title: Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light Subject: This work aimed to investigate how stimulation of donkey sperm with red LED light affects mitochondrial function. Keywords: sperm, red light stimulation, mitochondrial function, donkey, oligomycin A, FCCP Author: Marc Yeste Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Tue Dec 1 16:01:14 2020 CET ModDate: Tue Dec 1 18:23:05 2020 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 18 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 2300835 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QPKUYG+MinionPro-Regular Type 1 Custom yes yes no 34 0 DUIXQH+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 35 0 QMEHHD+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 36 0 CCVXXJ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 41 0 WBQIVR+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 42 0 JFQXWZ+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 43 0 ZYUAEV+RBLMI Type 1 Builtin yes yes no 148 0 APMKFX+MinionPro-It 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Title: Animals, Semen Samples, and Ethics Item: Title: Experimental Design Item: Title: Sperm Motility Children: Item: Title: Flow Cytometry Item: Title: Plasma Membrane Integrity Item: Title: Mitochondrial Membrane Potential Item: Title: Analysis of Intracellular ROS Levels: H2O2 and O2- Item: Title: Intracellular Calcium Levels Item: Title: Determination of Intracellular ATP Levels Item: Title: Determination of O2 Consumption Rate Item: Title: Determination of Cytochrome C Oxidase Activity Item: Title: Statistical Analysis Item: Title: Results Children: Item: Title: Effects of Red Light Stimulation on Plasma Membrane Integrity in the Presence or Absence of Either Omy A or FCCP Item: Title: Effects of Red Light Stimulation on Sperm Motility in the Presence or Absence of Either Omy A or FCCP Item: Title: Effects of Red Light Stimulation on Mitochondrial Membrane Potential in the Presence or Absence of Either Omy A or FCCP Item: Title: Effects of Red Light Stimulation on Intracellular ROS Levels in the Presence or Absence of Either Omy A or FCCP Item: Title: Effects of Red Light Stimulation on Intracellular Calcium Levels in the Presence or Absence of Either Omy A or FCCP Item: Title: Effects of Red Light Stimulation on Intracellular Levels of ATP and Oxygen Consumption in the Presence or Absence of Either Omy A or FCCP Item: Title: Effects of Red Light Stimulation on Cytochrome C Oxidase Activity in the Presence or Absence of Either Omy A or FCCP Item: Title: Discussion Item: Title: Conclusion Item: Title: Data Availability Statement Item: Title: Ethics Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QPKUYG+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: QPKUYG+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true 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Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light This work aimed to investigate how stimulation of donkey sperm with red LED light affects mitochondrial function. Marc Yeste sperm red light stimulation mitochondrial function donkey oligomycin A FCCP 2020-12-01T20:31:14+05:30 2020-12-01T20:31:14+05:30 2020-12-01T22:53:05+05:30 2020-12-01T22:53:05+05:30 LaTeX with hyperref package uuid:c9b6ce41-8d1d-4188-8888-d1d1d7a3efd7 uuid:1cd99574-cb4a-4772-819c-add9482511de Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 43, 718, 270, 754 Annotation: Subtype: Link Rect: 474, 736, 551, 745 Annotation: Subtype: Link Rect: 457, 727, 551, 737 Annotation: Subtype: Link Rect: 454, 718, 551, 729 Annotation: Subtype: Link Rect: 86, 495, 160, 505 Annotation: Subtype: Link Rect: 76, 67, 160, 74 Annotation: Subtype: Link Rect: 521, 677, 551, 707 Annotation: Subtype: Link Rect: 176, 516, 551, 629 Annotation: Subtype: Link Rect: 259, 87, 315, 99 ActionDest: 813 Annotation: Subtype: Link Rect: 319, 87, 339, 99 ActionDest: 813 Annotation: Subtype: Link Rect: 343, 87, 395, 99 ActionDest: 813 Annotation: Subtype: Link Rect: 399, 87, 419, 99 ActionDest: 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