0a1e48ac6916bcb8df5702ae0b35ddc5 pmc_33371314.pdf 2f9cd9a1f6806015926d8c39e55d86498ce072d8 pmc_33371314.pdf 726aaf73003525874b17c0c166f4cf47bb37fc7228dbc28a7915a87363f169e1 pmc_33371314.pdf Title: Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis Subject: Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, smear microscopy and X-Rays. Oral mucosa swabs collected on PrimeStore-MTM, stored at room temperature if tested within 30 days or at -20 C if examined at a later time. RT-PCR was performed to detect M. tuberculosis DNA. Eighty patients had bacteriologically-confirmed TB, 34 had bacteriologically-negative TB (negative tests but abnormal X-rays) and 152 were considered not to have TB (not TB). Oral swabs RT-PCR were positive in 29/80 (36.3%) bacteriologically-confirmed, 9/34 (26.5%) bacteriologically-negative and 29/152 (19.1%) not TB. The yield varied among samples stored for less and more than 30 days (p = 0.013) from 61% (11/18) and 29% (18/62) among bacteriologically confirmed, and 30.8% (4/13) and 23.8% (5/21) among bacteriologically-negative participants. Among not TB patients, the specificity was 80.9% (123/152), being 78.3% (18/23) among samples stored less than 30 days and 81.4% (105/129) among samples stored for more than 30 days (p = 0.46). The detection of M. tuberculosis in oral mucosa samples is feasible, but storage conditions may affect the yield. Keywords: oral swab; diagnosis; real-time PCR Author: Barbara Molina-Moya, Nelly Ciobanu, Marta Hernandez, Cristina Prat-Aymerich, Valeriu Crudu, Emily R. Adams, Alexandru Codreanu, Derek J. Sloan, Luis E. Cuevas and Jose Dominguez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Dec 22 08:08:28 2020 CET ModDate: Tue Dec 22 08:15:52 2020 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 235757 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- NXPYWR+Pxsy Type 1 Builtin yes yes no 10 0 VUVBFS+VnURWPalladioL Type 1 Custom yes yes no 14 0 URNFIR+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 ZSEIDT+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 GSMHDM+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 RPDRHF+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 OFLXAT+Rpxr Type 1 Builtin yes yes no 36 0 CUAJJQ+Pxbsy Type 1 Builtin yes yes no 40 0 XPUSBJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 51 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 73 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 77 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 81 0 HVOROU+Rpxb Type 1 Builtin yes yes no 91 0 YWGLGW+Rpxi Type 1 Builtin yes yes no 107 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 111 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:47:44 CEST RepresentationInformation: pmc_33371314.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 11:43:40 CEST Size: 235757 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 173 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Info: Title: Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis Author: Barbara Molina-Moya, Nelly Ciobanu, Marta Hernandez, Cristina Prat-Aymerich, Valeriu Crudu, Emily R. Adams, Alexandru Codreanu, Derek J. Sloan, Luis E. Cuevas and Jose Dominguez Subject: Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, smear microscopy and X-Rays. Oral mucosa swabs collected on PrimeStore-MTM, stored at room temperature if tested within 30 days or at -20 C if examined at a later time. RT-PCR was performed to detect M. tuberculosis DNA. Eighty patients had bacteriologically-confirmed TB, 34 had bacteriologically-negative TB (negative tests but abnormal X-rays) and 152 were considered not to have TB (not TB). Oral swabs RT-PCR were positive in 29/80 (36.3%) bacteriologically-confirmed, 9/34 (26.5%) bacteriologically-negative and 29/152 (19.1%) not TB. The yield varied among samples stored for less and more than 30 days (p = 0.013) from 61% (11/18) and 29% (18/62) among bacteriologically confirmed, and 30.8% (4/13) and 23.8% (5/21) among bacteriologically-negative participants. Among not TB patients, the specificity was 80.9% (123/152), being 78.3% (18/23) among samples stored less than 30 days and 81.4% (105/129) among samples stored for more than 30 days (p = 0.46). The detection of M. tuberculosis in oral mucosa samples is feasible, but storage conditions may affect the yield. 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