987587f3191a534902e242af35fb77da pmc_32092878.pdf 0922e4b4928e039abbec818900c978f7fc3b1b24 pmc_32092878.pdf a07f531b9e5c57dfe104bdbc26cf54df2ebf45e7fdc523beeab6a82c32b75f70 pmc_32092878.pdf Title: Effects of Cannabis Use on the Protein and Lipid Profile of Olfactory Neuroepithelium Cells from Schizophrenia Patients Studied by Synchrotron-Based FTIR Spectroscopy Subject: Schizophrenia (SCZ) is a neurodevelopmental disorder with a high genetic component, but the presence of environmental stressors can be important for its onset and progression. Cannabis use can be a major risk factor for developing SCZ. However, despite the available data on the neurobiological underpinnings of SCZ, there is an important lack of studies in human neuronal tissue and living cells addressing the effects of cannabis in SCZ patients. In this study, we analysed the most relevant bio-macromolecular constituents in olfactory neuroepithelium (ON) cells of healthy controls non-cannabis users, healthy cannabis users, SCZ patients non-cannabis users, and SCZ patients cannabis users using Synchrotron Radiation-Fourier Transform Infrared (SR-FTIR) spectrometry and microscopy. Our results revealed that SCZ patients non-cannabis users, and healthy cannabis users exhibit similar alterations in the macromolecular profile of ON cells, including disruption in lipid composition, increased lipid membrane renewal rate and lipid peroxidation, altered proteins containing more -sheet structures, and showed an increase in DNA and histone methylation. Notably, these alterations were not observed in SCZ patients who use cannabis regularly. These data suggest a differential effect of cannabis in healthy controls and in SCZ patients in terms of the macromolecular constituents of ON cells. Keywords: schizophrenia; olfactory neuroepithelium; SR-FTIR; cannabis Author: Sergi Saladrigas-Manjón, Tanja Dučić, Liliana Galindo, Cristina Fernández-Avilés, Víctor Pérez, Rafael de la Torre and Patricia Robledo Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Wed Feb 19 11:20:42 2020 CET ModDate: Wed Feb 19 11:20:42 2020 CET Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1733834 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AGCOES+URWPalladioL-Ital Type 1 Custom yes yes no 88 0 BKAJAH+URWPalladioL-Bold Type 1 Custom yes yes no 89 0 LZDGTK+Rpxb Type 1 Builtin yes yes no 90 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 91 0 YWJXXD+URWPalladioL-Roma Type 1 Custom yes yes no 92 0 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yes 410 0 CODIBE+PalatinoLinotype TrueType WinAnsi yes yes no 411 0 CODIMB+TimesNewRoman CID TrueType Identity-H yes yes yes 412 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:14:36 CEST RepresentationInformation: pmc_32092878.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:44:13 CEST Size: 1733834 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはの〠 Item: Title: きちぴづひどちぬ〠ちのつ〠きづぴとはつび〠 Children: Item: Title: こふぢなづっぴび〠ちのつ〠こぴふつべ〠いづびどでの〠 Item: Title: ぃぬどのどっちぬ〠ちのつ〠うふのっぴどはのちぬ〠ぁびびづびびねづのぴ〠 Item: 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