8f392f72a77c46befd8a8f203bf8ed62 pmc_32140111.pdf f0633e1a8822c6a5799d6489eae4e88c58ba39be pmc_32140111.pdf 9638ea97d74e0aff3668e828afb5d22f4a15eccf4020ea8b8692821fdb46e947 pmc_32140111.pdf Title: A Transcriptome Analysis: Various Reasons of Adverse Pregnancy Outcomes Caused by Acute Toxoplasma gondii Infection Subject: Background: Toxoplasma gondii (T. gondii) is an obligate intracellular parasite, which can affect the pregnancy outcomes in infected females by damaging the uterus, and the intrauterine environment as well as and the hypothalamus resulting in hormonal imbalance. Keywords: T. gondii, RNA-Seq, acute infection, abnormal pregnancy, DEGs Author: Xiu-Xiang Zhang Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Mon Feb 17 12:27:16 2020 CET ModDate: Mon Feb 17 13:36:26 2020 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 4050983 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PDSHQN+MinionPro-Regular Type 1 Custom yes yes no 28 0 VCFUTZ+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 29 0 MWTNIF+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 30 0 HBLJAG+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 35 0 TTGANQ+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 36 0 SGULQI+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 37 0 COYNAT+MinionPro-It Type 1 Custom yes yes no 106 0 WEGEYF+MinionPro-Bold Type 1 Custom yes yes no 108 0 RYFJSZ+MTSYN Type 1 Builtin yes yes no 168 0 OFLPKB+MTGU Type 1 Builtin yes yes no 172 0 VXWSEY+RBLMI Type 1 Builtin yes yes no 176 0 XDWDPG+MTSYB Type 1 Builtin yes yes no 242 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-11-18 06:54:41 CET RepresentationInformation: pmc_32140111.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-03-16 03:50:49 CET Size: 4050983 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 698 FreeObjects: 7 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: A Transcriptome Analysis: Various Reasons of Adverse Pregnancy Outcomes Caused by Acute Toxoplasma gondii Infection Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Animals, Parasite Challenge and Sample Collection Item: Title: mRNA Library Preparation and Sequencing Item: Title: Processing of RNA-Seq Data Item: Title: Identification and Bioinformatic Analysis of Differentially Expressed Genes Item: Title: Validation of RNA-Seq Results by Quantitative Real-Time PCR (qRT-PCR) Analysis Item: Title: Validations of RNA-Seq Results by Western Blotting Item: Title: Results Children: Item: Title: Identification of Expressed Transcripts in the Mouse Uterus Transcriptome Item: Title: GO Enrichment of Differentially Expressed Genes Item: Title: KEGG Enrichment Analysis Item: Title: Validation of Ten Differentially Expressed Genes by qRT-PCR Item: Title: Verification of Hsd11b2 and Ifitm1 Expression Levels by Western Blotting Item: Title: Discussion Item: Title: Conclusion Item: Title: Data Availability Statement Item: Title: Ethics Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: A Transcriptome Analysis: Various Reasons of Adverse Pregnancy Outcomes Caused by Acute Toxoplasma gondii Infection Author: Xiu-Xiang Zhang Subject: Background: Toxoplasma gondii (T. gondii) is an obligate intracellular parasite, which can affect the pregnancy outcomes in infected females by damaging the uterus, and the intrauterine environment as well as and the hypothalamus resulting in hormonal imbalance. Keywords: T. gondii, RNA-Seq, acute infection, abnormal pregnancy, DEGs Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Mon Feb 17 12:57:16 CET 2020 ModDate: Mon Feb 17 14:06:26 CET 2020 ID: 0x57d4e29209c70c900730ba561382fc64, 0x8ffb0becd2e30c42aa96a9092bbc2d40 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: VXWSEY+RBLMI FontSubset: true FirstChar: 49 LastChar: 62 FontDescriptor: FontName: VXWSEY+RBLMI Flags: Symbolic FontBBox: -11, -230, 987, 750 FontFile: true Font: BaseFont: XDWDPG+MTSYB FontSubset: true FirstChar: 48 LastChar: 48 FontDescriptor: FontName: XDWDPG+MTSYB Flags: Symbolic FontBBox: -16, -935, 1043, 896 FontFile: true Font: BaseFont: HBLJAG+HelveticaNeueLTStd-BdIt FontSubset: true FirstChar: 42 LastChar: 121 FontDescriptor: FontName: HBLJAG+HelveticaNeueLTStd-BdIt Flags: Symbolic FontBBox: -166, -218, 1129, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TTGANQ+HelveticaNeueLTStd-LtIt FontSubset: true FirstChar: 2 LastChar: 243 FontDescriptor: FontName: TTGANQ+HelveticaNeueLTStd-LtIt Flags: Symbolic FontBBox: -165, -214, 1099, 951 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: SGULQI+HelveticaNeueLTStd-MdIt FontSubset: true FirstChar: 42 LastChar: 135 FontDescriptor: FontName: SGULQI+HelveticaNeueLTStd-MdIt Flags: Symbolic FontBBox: -165, -218, 1125, 947 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: RYFJSZ+MTSYN FontSubset: true FirstChar: 0 LastChar: 67 FontDescriptor: FontName: RYFJSZ+MTSYN Flags: Symbolic FontBBox: 0, -954, 1043, 900 FontFile: true Font: BaseFont: COYNAT+MinionPro-It FontSubset: true FirstChar: 40 LastChar: 169 FontDescriptor: FontName: COYNAT+MinionPro-It Flags: Symbolic FontBBox: -201, -360, 1684, 919 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: PDSHQN+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 243 FontDescriptor: FontName: PDSHQN+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WEGEYF+MinionPro-Bold FontSubset: true FirstChar: 3 LastChar: 121 FontDescriptor: FontName: WEGEYF+MinionPro-Bold Flags: Symbolic FontBBox: -318, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: OFLPKB+MTGU FontSubset: true FirstChar: 98 LastChar: 109 FontDescriptor: FontName: OFLPKB+MTGU Flags: Symbolic FontBBox: 25, -213, 839, 683 FontFile: true Font: BaseFont: VCFUTZ+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 2 LastChar: 150 FontDescriptor: FontName: VCFUTZ+HelveticaNeueLTStd-Bd Flags: Symbolic FontBBox: -166, -218, 1078, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MWTNIF+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: MWTNIF+HelveticaNeueLTStd-Lt Flags: Symbolic FontBBox: -166, -214, 1050, 967 FontFile: true EncodingDictionary: Differences: true XMP: T. gondii, RNA-Seq, acute infection, abnormal pregnancy, DEGs pdfTeX-1.40.15 1.5 application/pdf fphys-11-00115.tex A Transcriptome Analysis: Various Reasons of Adverse Pregnancy Outcomes Caused by Acute Toxoplasma gondii Infection Background: Toxoplasma gondii (T. gondii) is an obligate intracellular parasite, which can affect the pregnancy outcomes in infected females by damaging the uterus, and the intrauterine environment as well as and the hypothalamus resulting in hormonal imbalance. Xiu-Xiang Zhang Xiu-Ming Li and Zi-Guo Yuan 2020-02-17T16:57:16+05:30 T. gondii RNA-Seq acute infection abnormal pregnancy DEGs 2020-02-17T16:57:16+05:30 2020-02-17T18:06:26+05:30 2020-02-17T18:06:26+05:30 LaTeX with hyperref package uuid:90a88d7f-2620-4a8d-bfdc-4133ea8d18d7 uuid:b0788b4e-d40e-499c-9c0b-076904a4826d Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 43, 718, 147, 754 Annotation: Subtype: Link Rect: 474, 736, 551, 745 Annotation: Subtype: Link Rect: 462, 727, 551, 737 Annotation: Subtype: Link Rect: 453, 718, 551, 729 Annotation: Subtype: Link Rect: 86, 529, 160, 539 Annotation: Subtype: Link Rect: 75, 66, 160, 74 Annotation: Subtype: Link Rect: 521, 677, 551, 707 Annotation: Subtype: Link Rect: 176, 511, 551, 601 Annotation: Subtype: Link Rect: 176, 490, 218, 501 Annotation: Subtype: Link Rect: 326, 490, 414, 501 Annotation: Subtype: Link Rect: 389, 480, 457, 490 Annotation: Subtype: Link Rect: 194, 469, 248, 481 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 198, 541, 262, 552 ActionDest: 504 Annotation: Subtype: Link Rect: 267, 541, 287, 552 ActionDest: 504 Annotation: Subtype: Link Rect: 104, 461, 143, 472 ActionDest: 504 Annotation: Subtype: Link Rect: 146, 461, 167, 472 ActionDest: 504 Annotation: Subtype: Link Rect: 47, 393, 131, 403 ActionDest: 544 Annotation: Subtype: Link Rect: 133, 393, 153, 403 ActionDest: 544 Annotation: Subtype: Link Rect: 272, 369, 292, 380 ActionDest: 544 Annotation: Subtype: Link Rect: 43, 358, 68, 369 ActionDest: 544 Annotation: Subtype: Link Rect: 75, 358, 95, 369 ActionDest: 544 Annotation: Subtype: Link Rect: 105, 358, 155, 369 ActionDest: 544 Annotation: Subtype: Link Rect: 163, 358, 183, 369 ActionDest: 544 Annotation: Subtype: Link Rect: 89, 300, 140, 312 ActionDest: 544 Annotation: Subtype: Link Rect: 147, 300, 167, 312 ActionDest: 544 Annotation: Subtype: Link Rect: 169, 243, 236, 254 ActionDest: 544 Annotation: Subtype: Link Rect: 240, 243, 260, 254 ActionDest: 544 Annotation: Subtype: Link Rect: 151, 220, 223, 231 ActionDest: 544 Annotation: Subtype: Link Rect: 229, 220, 249, 231 ActionDest: 544 Annotation: Subtype: Link Rect: 113, 186, 156, 197 ActionDest: 504 Annotation: Subtype: Link Rect: 160, 186, 181, 197 ActionDest: 504 Annotation: Subtype: Link Rect: 189, 186, 246, 197 ActionDest: 504 Annotation: Subtype: Link Rect: 252, 186, 272, 197 ActionDest: 504 Annotation: Subtype: Link Rect: 321, 591, 370, 603 ActionDest: 504 Annotation: Subtype: Link Rect: 374, 591, 394, 603 ActionDest: 504 Annotation: Subtype: Link Rect: 363, 569, 464, 580 ActionDest: 544 Annotation: Subtype: Link Rect: 468, 569, 489, 580 ActionDest: 544 Annotation: Subtype: Link Rect: 435, 547, 491, 557 ActionDest: 504 Annotation: Subtype: Link Rect: 494, 547, 515, 557 ActionDest: 504 Annotation: Subtype: Link Rect: 517, 547, 551, 557 ActionDest: 544 Annotation: Subtype: Link Rect: 302, 534, 322, 546 ActionDest: 544 Annotation: Subtype: Link Rect: 325, 534, 345, 546 ActionDest: 544 Annotation: Subtype: Link Rect: 528, 511, 551, 523 ActionDest: 504 Annotation: Subtype: Link Rect: 302, 500, 323, 511 ActionDest: 504 Annotation: Subtype: Link Rect: 325, 500, 346, 511 ActionDest: 504 Annotation: Subtype: Link Rect: 337, 454, 381, 465 ActionDest: 504 Annotation: Subtype: Link Rect: 383, 454, 404, 465 ActionDest: 504 Annotation: Subtype: Link Rect: 409, 420, 478, 431 ActionDest: 544 Annotation: Subtype: Link Rect: 484, 420, 504, 431 ActionDest: 544 Annotation: Subtype: Link Rect: 509, 420, 549, 431 ActionDest: 544 Annotation: Subtype: Link Rect: 302, 408, 322, 420 ActionDest: 544 Annotation: Subtype: Link Rect: 521, 305, 551, 316 ActionDest: 544 Annotation: Subtype: Link Rect: 302, 294, 326, 305 ActionDest: 544 Annotation: Subtype: Link Rect: 332, 294, 352, 305 ActionDest: 544 Annotation: Subtype: Link Rect: 430, 294, 486, 305 ActionDest: 504 Annotation: Subtype: Link Rect: 493, 294, 513, 305 ActionDest: 504 Annotation: Subtype: Link Rect: 514, 271, 551, 282 ActionDest: 504 Annotation: Subtype: Link Rect: 305, 259, 331, 271 ActionDest: 504 Annotation: Subtype: Link Rect: 399, 213, 438, 225 ActionDest: 504 Annotation: Subtype: Link Rect: 443, 213, 468, 225 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 202, 345, 240, 357 ActionDest: 200 Annotation: Subtype: Link Rect: 516, 486, 551, 498 ActionDest: 544 Annotation: Subtype: Link Rect: 302, 475, 322, 486 ActionDest: 544 Annotation: Subtype: Link Rect: 325, 475, 345, 486 ActionDest: 544 Annotation: Subtype: Link Rect: 521, 390, 551, 401 ActionDest: 504 Annotation: Subtype: Link Rect: 302, 379, 349, 390 ActionDest: 504 Annotation: Subtype: Link Rect: 355, 379, 375, 390 ActionDest: 504 Annotation: Subtype: Link Rect: 444, 367, 476, 378 ActionDest: 504 Annotation: Subtype: Link Rect: 480, 367, 501, 378 ActionDest: 504 Annotation: Subtype: Link Rect: 546, 310, 551, 321 ActionDest: 159 Annotation: Subtype: Link Rect: 526, 275, 531, 287 ActionDest: 159 Annotation: Subtype: Link Rect: 546, 241, 551, 252 ActionDest: 159 Annotation: Subtype: Link Rect: 305, 85, 399, 98 Annotation: Subtype: Link Rect: 305, 75, 384, 87 Annotation: Subtype: Link Rect: 305, 64, 395, 77 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 0, 412, 2, 424 ActionDest: 5 Annotation: Subtype: Link Rect: 0, 412, 2, 424 ActionDest: 5 Annotation: Subtype: Link Rect: 113, 304, 143, 315 ActionDest: 239 Annotation: Subtype: Link Rect: 200, 178, 290, 191 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 167, 64, 178 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 108, 387, 139, 398 ActionDest: 262 Annotation: Subtype: Link Rect: 259, 192, 290, 204 ActionDest: 262 Annotation: Subtype: Link Rect: 216, 147, 251, 158 ActionDest: 273 Annotation: Subtype: Link Rect: 257, 147, 292, 158 ActionDest: 313 Annotation: Subtype: Link Rect: 186, 64, 224, 76 ActionDest: 365 Annotation: Subtype: Link Rect: 330, 307, 372, 318 ActionDest: 365 Annotation: Subtype: Link Rect: 458, 180, 499, 192 ActionDest: 365 Annotation: Subtype: Link Rect: 509, 122, 545, 134 ActionDest: 401 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 141, 87, 182, 99 ActionDest: 417 Annotation: Subtype: Link Rect: 354, 191, 395, 203 ActionDest: 417 Annotation: Subtype: Link Rect: 340, 65, 381, 77 ActionDest: 417 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 84, 128, 119, 139 ActionDest: 438 Annotation: Subtype: Link Rect: 511, 683, 545, 694 ActionDest: 438 Annotation: Subtype: Link Rect: 361, 419, 412, 431 ActionDest: 504 Annotation: Subtype: Link Rect: 418, 419, 438, 431 ActionDest: 504 Annotation: Subtype: Link Rect: 444, 419, 549, 431 ActionDest: 504 Annotation: Subtype: Link Rect: 302, 408, 322, 419 ActionDest: 504 Annotation: Subtype: Link Rect: 448, 374, 549, 385 ActionDest: 504 Annotation: Subtype: Link Rect: 302, 362, 322, 373 ActionDest: 504 Annotation: Subtype: Link Rect: 399, 305, 446, 316 ActionDest: 544 Annotation: Subtype: Link Rect: 451, 305, 471, 316 ActionDest: 544 Annotation: Subtype: Link Rect: 499, 293, 551, 305 ActionDest: 504 Annotation: Subtype: Link Rect: 305, 282, 326, 293 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 98, 352, 147, 364 ActionDest: 544 Annotation: Subtype: Link Rect: 152, 352, 172, 364 ActionDest: 544 Annotation: Subtype: Link Rect: 224, 341, 260, 352 ActionDest: 465 Annotation: Subtype: Link Rect: 184, 284, 227, 296 ActionDest: 544 Annotation: Subtype: Link Rect: 231, 284, 251, 296 ActionDest: 544 Annotation: Subtype: Link Rect: 222, 261, 265, 272 ActionDest: 504 Annotation: Subtype: Link Rect: 270, 261, 290, 272 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 249, 105, 261 ActionDest: 544 Annotation: Subtype: Link Rect: 108, 249, 128, 261 ActionDest: 544 Annotation: Subtype: Link Rect: 47, 238, 95, 249 ActionDest: 504 Annotation: Subtype: Link Rect: 100, 238, 121, 249 ActionDest: 504 Annotation: Subtype: Link Rect: 47, 146, 89, 158 ActionDest: 544 Annotation: Subtype: Link Rect: 93, 146, 113, 158 ActionDest: 544 Annotation: Subtype: Link Rect: 79, 123, 121, 135 ActionDest: 504 Annotation: Subtype: Link Rect: 127, 123, 147, 135 ActionDest: 504 Annotation: Subtype: Link Rect: 305, 387, 368, 398 ActionDest: 544 Annotation: Subtype: Link Rect: 373, 387, 393, 398 ActionDest: 544 Annotation: Subtype: Link Rect: 469, 376, 521, 387 ActionDest: 504 Annotation: Subtype: Link Rect: 525, 376, 545, 387 ActionDest: 504 Annotation: Subtype: Link Rect: 369, 352, 411, 364 ActionDest: 504 Annotation: Subtype: Link Rect: 417, 352, 437, 364 ActionDest: 504 Annotation: Subtype: Link Rect: 305, 238, 356, 249 ActionDest: 544 Annotation: Subtype: Link Rect: 361, 238, 381, 249 ActionDest: 544 Annotation: Subtype: Link Rect: 518, 227, 549, 238 ActionDest: 504 Annotation: Subtype: Link Rect: 302, 216, 322, 226 ActionDest: 504 Annotation: Subtype: Link Rect: 326, 216, 385, 226 ActionDest: 504 Annotation: Subtype: Link Rect: 388, 216, 408, 226 ActionDest: 504 Annotation: Subtype: Link Rect: 469, 204, 523, 215 ActionDest: 504 Annotation: Subtype: Link Rect: 525, 204, 545, 215 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 209, 116, 260, 127 ActionDest: 544 Annotation: Subtype: Link Rect: 267, 116, 287, 127 ActionDest: 544 Annotation: Subtype: Link Rect: 440, 220, 501, 230 ActionDest: 544 Annotation: Subtype: Link Rect: 503, 220, 523, 230 ActionDest: 544 Annotation: Subtype: Link Rect: 526, 93, 551, 104 ActionDest: 504 Annotation: Subtype: Link Rect: 305, 81, 326, 93 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 267, 122, 292, 133 ActionDest: 544 Annotation: Subtype: Link Rect: 43, 110, 67, 121 ActionDest: 544 Annotation: Subtype: Link Rect: 74, 110, 95, 121 ActionDest: 544 Annotation: Subtype: Link Rect: 494, 226, 551, 237 ActionDest: 544 Annotation: Subtype: Link Rect: 302, 215, 333, 225 ActionDest: 544 Annotation: Subtype: Link Rect: 339, 215, 359, 225 ActionDest: 544 Annotation: Subtype: Link Rect: 364, 215, 418, 225 ActionDest: 544 Annotation: Subtype: Link Rect: 424, 215, 444, 225 ActionDest: 544 Annotation: Subtype: Link Rect: 456, 77, 507, 88 ActionDest: 544 Annotation: Subtype: Link Rect: 510, 77, 530, 88 ActionDest: 544 Annotation: Subtype: Link Rect: 385, 66, 435, 77 ActionDest: 504 Annotation: Subtype: Link Rect: 437, 66, 458, 77 ActionDest: 504 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 481, 145, 551, 156 Annotation: Subtype: Link Rect: 302, 133, 451, 144 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 355, 497, 551, 509 Annotation: Subtype: Link Rect: 302, 486, 460, 497 Annotation: Subtype: Link Rect: 158, 425, 261, 434 Annotation: Subtype: Link Rect: 53, 358, 155, 368 Annotation: Subtype: Link Rect: 195, 330, 272, 339 Annotation: Subtype: Link Rect: 123, 292, 226, 301 Annotation: Subtype: Link Rect: 82, 264, 182, 273 Annotation: Subtype: Link Rect: 118, 141, 204, 150 Annotation: Subtype: Link Rect: 248, 122, 293, 131 Annotation: Subtype: Link Rect: 53, 112, 118, 122 Annotation: Subtype: Link Rect: 140, 66, 229, 74 Annotation: Subtype: Link Rect: 396, 435, 496, 443 Annotation: Subtype: Link Rect: 410, 396, 489, 405 Annotation: Subtype: Link Rect: 355, 331, 455, 339 Annotation: Subtype: Link Rect: 341, 293, 441, 301 Annotation: Subtype: Link Rect: 458, 197, 527, 207 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 100, 698, 199, 706 Annotation: Subtype: Link Rect: 128, 574, 207, 583 Annotation: Subtype: Link Rect: 239, 545, 293, 555 Annotation: Subtype: Link Rect: 53, 537, 84, 545 Annotation: Subtype: Link Rect: 279, 517, 293, 526 Annotation: Subtype: Link Rect: 53, 508, 148, 517 Annotation: Subtype: Link Rect: 152, 470, 221, 479 Annotation: Subtype: Link Rect: 82, 319, 182, 328 Annotation: Subtype: Link Rect: 127, 271, 236, 280 Annotation: Subtype: Link Rect: 426, 659, 508, 668 Annotation: Subtype: Link Rect: 386, 594, 453, 602 Annotation: Subtype: Link Rect: 506, 536, 551, 545 Annotation: Subtype: Link Rect: 312, 528, 358, 536 Annotation: Subtype: Link Rect: 341, 480, 431, 489 Annotation: Subtype: Link Rect: 524, 318, 551, 328 Annotation: Subtype: Link Rect: 302, 309, 334, 318 Annotation: Subtype: Link Rect: 335, 309, 370, 318 Annotation: Subtype: Link Rect: 371, 309, 395, 318 Annotation: Subtype: Link Rect: 396, 309, 424, 318 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 430, 29, 551, 38 Checksum: 6debb09b Type: CRC32 Checksum: 8f392f72a77c46befd8a8f203bf8ed62 Type: MD5 Checksum: f0633e1a8822c6a5799d6489eae4e88c58ba39be Type: SHA-1