1bab0ccb0ce5e5240c3f0dde455e6e31 pmc_32150818.pdf 10761d1b6d34894c3e23c4fefe6ecbdb76eb0334 pmc_32150818.pdf dec5dd2ad7f0adfc4ad2afac02fc2dd212e11fdbb98ab1694d9095e7300ebdd4 pmc_32150818.pdf Title: Effects of Titanium Dioxide Nanoparticles on the Hprt Gene Mutations in V79 Hamster Cells Subject: The genotoxicity of anatase/rutile TiO2 nanoparticles (TiO2 NPs, NM105 at 3, 15 and 75 g/cm2) was assessed with the mammalian in-vitro Hypoxanthine guanine phosphoribosyl transferase (Hprt) gene mutation test in Chinese hamster lung (V79) fibroblasts after 24 h exposure. Two dispersion procedures giving different size distribution and dispersion stability were used to investigate whether the effects of TiO2 NPs depend on the state of agglomeration. TiO2 NPs were fully characterised in the previous European FP7 projects NanoTEST and NanoREG2. Uptake of TiO2 NPs was measured by transmission electron microscopy (TEM). TiO2 NPs were found in cytoplasmic vesicles, as well as close to the nucleus. The internalisation of TiO2 NPs did not depend on the state of agglomeration and dispersion used. The cytotoxicity of TiO2 NPs was measured by determining both the relative growth activity (RGA) and the plating efficiency (PE). There were no substantial effects of exposure time (24, 48 and 72 h), although a tendency to lower RGA at longer exposure was observed. No significant difference in PE values and no increases in the Hprt gene mutant frequency were found in exposed relative to unexposed cultures in spite of evidence of uptake of NPs by cells. Keywords: titanium dioxide nanoparticles; V79 cells; genotoxicity; Hprt Author: Alena Kazimirova, Naouale El Yamani, Laura Rubio, Alba García-Rodríguez, Magdalena Barancokova, Ricard Marcos and Maria Dusinska Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Mar 5 08:57:46 2020 CET ModDate: Thu Mar 5 08:57:46 2020 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1075553 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SVJCOC+URWPalladioL-Ital Type 1 Custom yes yes no 102 0 QYTDDH+URWPalladioL-Bold Type 1 Custom yes yes no 103 0 LZDGTK+Rpxb Type 1 Builtin yes yes no 104 0 OGJGYD+URWPalladioL-BoldItal Type 1 Custom yes yes no 105 0 WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes no 106 0 BLIIJN+URWPalladioL-Roma Type 1 Custom yes 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369 0 QBUZPS+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 370 0 UPVSTK+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 371 0 GRYRFM+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 372 0 TimesNewRomanPSMT TrueType WinAnsi no no no 373 0 ArialNarrow TrueType WinAnsi no no no 374 0 ArialMT TrueType WinAnsi no no no 375 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:45:15 CEST RepresentationInformation: pmc_32150818.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 11:44:17 CEST Size: 1075553 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 3 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぎちのはばちひぴどっぬづぜ〴ぜ〰ぃとちひちっぴづひどぺちぴどはの〬ぜ〴ぜ〰いどびばづひびどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぃづぬぬぜ〴ぜ〰ぅへばはびふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぅへぴひどのびどっぜ〴ぜ〰ぐひはばづひぴどづびぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬふぬちひぜ〴ぜ〰さばぴちにづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどぶづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰ぜ〵ぜ〰げぇぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぐぬちぴどのでぜ〴ぜ〰ぅててどっどづのっべぜ〴ぜ〰ぜ〵ぜ〰ぐぅぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぃづぬぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぎちのはばちひぴどっぬづぜ〴ぜ〰ぃとちひちっぴづひどぺちぴどはの〬ぜ〴ぜ〰いどびばづひびどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぃづぬぬぜ〴ぜ〰ぅへばはびふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぅへぴひどのびどっぜ〴ぜ〰ぐひはばづひぴどづびぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬふぬちひぜ〴ぜ〰さばぴちにづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどぶづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰ぜ〵ぜ〰げぇぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぐぬちぴどのでぜ〴ぜ〰ぅててどっどづのっべぜ〴ぜ〰ぜ〵ぜ〰ぐぅぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぎちのはばちひぴどっぬづぜ〴ぜ〰ぃとちひちっぴづひどぺちぴどはの〬ぜ〴ぜ〰いどびばづひびどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぃづぬぬぜ〴ぜ〰ぅへばはびふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぅへぴひどのびどっぜ〴ぜ〰ぐひはばづひぴどづびぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬふぬちひぜ〴ぜ〰さばぴちにづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどぶづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰ぜ〵ぜ〰げぇぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぐぬちぴどのでぜ〴ぜ〰ぅててどっどづのっべぜ〴ぜ〰ぜ〵ぜ〰ぐぅぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぅへぴひどのびどっぜ〴ぜ〰ぐひはばづひぴどづびぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬふぬちひぜ〴ぜ〰さばぴちにづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどぶづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰ぜ〵ぜ〰げぇぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぐぬちぴどのでぜ〴ぜ〰ぅててどっどづのっべぜ〴ぜ〰ぜ〵ぜ〰ぐぅぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃづぬぬふぬちひぜ〴ぜ〰さばぴちにづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどぶづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰ぜ〵ぜ〰げぇぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぐぬちぴどのでぜ〴ぜ〰ぅててどっどづのっべぜ〴ぜ〰ぜ〵ぜ〰ぐぅぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづぬちぴどぶづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰ぜ〵ぜ〰げぇぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぐぬちぴどのでぜ〴ぜ〰ぅててどっどづのっべぜ〴ぜ〰ぜ〵ぜ〰ぐぅぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぐぬちぴどのでぜ〴ぜ〰ぅててどっどづのっべぜ〴ぜ〰ぜ〵ぜ〰ぐぅぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: えばひぴぜ〴ぜ〰きちねねちぬどちのぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ごどく〲ぜ〴ぜ〰ぃとちひちっぴづひどぺちぴどはの〬ぜ〴ぜ〰ぅへぴひどのびどっぜ〴ぜ〰ぐひはばづひぴどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰さばぴちにづぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰きづちびふひづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぴとづぜ〴ぜ〰ごぅきぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰はのぜ〴ぜ〰ざ〷〹〭〴ぜ〴ぜ〰ぃづぬぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰きちねねちぬどちのぜ〴ぜ〰えばひぴぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべ〬ぜ〴ぜ〰ぴとづぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰ぁてぴづひぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰ぅへばはびふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ごどく〲ぜ〴ぜ〰ぃとちひちっぴづひどぺちぴどはの〬ぜ〴ぜ〰ぅへぴひどのびどっぜ〴ぜ〰ぐひはばづひぴどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰さばぴちにづぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰きづちびふひづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぴとづぜ〴ぜ〰ごぅきぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰はのぜ〴ぜ〰ざ〷〹〭〴ぜ〴ぜ〰ぃづぬぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰きちねねちぬどちのぜ〴ぜ〰えばひぴぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべ〬ぜ〴ぜ〰ぴとづぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰ぁてぴづひぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰ぅへばはびふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: さばぴちにづぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰きづちびふひづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぴとづぜ〴ぜ〰ごぅきぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰はのぜ〴ぜ〰ざ〷〹〭〴ぜ〴ぜ〰ぃづぬぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰きちねねちぬどちのぜ〴ぜ〰えばひぴぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべ〬ぜ〴ぜ〰ぴとづぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰ぁてぴづひぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰ぅへばはびふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃべぴはぴはへどっぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰はのぜ〴ぜ〰ざ〷〹〭〴ぜ〴ぜ〰ぃづぬぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰きちねねちぬどちのぜ〴ぜ〰えばひぴぜ〴ぜ〰ぇづのづぜ〴ぜ〰きふぴちぴどはのぜ〴ぜ〰ぁびびちべ〬ぜ〴ぜ〰ぴとづぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰ぁてぴづひぜ〴ぜ〰ごどく〲ぜ〴ぜ〰ぎぐびぜ〴ぜ〰ぅへばはびふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: 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422, 624, 459, 635 Annotation: Subtype: Link Rect: 465, 624, 502, 635 Annotation: Subtype: Link Rect: 145, 586, 183, 596 Annotation: Subtype: Link Rect: 189, 586, 225, 596 Annotation: Subtype: Link Rect: 201, 547, 239, 557 Annotation: Subtype: Link Rect: 172, 508, 519, 519 Annotation: Subtype: Link Rect: 97, 495, 401, 506 Annotation: Subtype: Link Rect: 358, 469, 395, 480 Annotation: Subtype: Link Rect: 100, 444, 137, 454 Annotation: Subtype: Link Rect: 159, 418, 196, 428 Annotation: Subtype: Link Rect: 100, 379, 137, 389 Annotation: Subtype: Link Rect: 341, 340, 379, 351 Annotation: Subtype: Link Rect: 100, 315, 137, 325 Annotation: Subtype: Link Rect: 328, 275, 366, 286 Annotation: Subtype: Link Rect: 372, 275, 408, 286 Annotation: Subtype: Link Rect: 145, 237, 183, 247 Annotation: Subtype: Link Rect: 189, 237, 225, 247 Annotation: Subtype: Link Rect: 181, 199, 219, 209 Annotation: Subtype: Link Rect: 225, 199, 261, 209 Annotation: Subtype: Link Rect: 248, 160, 285, 170 Annotation: Subtype: Link Rect: 292, 160, 328, 170 Annotation: Subtype: Link Rect: 76, 118, 166, 149 Annotation: Subtype: Link Rect: 234, 112, 407, 123 Checksum: 86e8be34 Type: CRC32 Checksum: 1bab0ccb0ce5e5240c3f0dde455e6e31 Type: MD5 Checksum: 10761d1b6d34894c3e23c4fefe6ecbdb76eb0334 Type: SHA-1