654b7a2b7c70fe7a1e615f3c14ba1e41 pmc_32193445.pdf b08e69666f16700d6aa592aad7c80dd08305b443 pmc_32193445.pdf 1a174f3b7598b7cff4b463f0140098b91d3970533ad22f43268bc80ef4a955f1 pmc_32193445.pdf Title: Mixtures of prion substrains in natural scrapie cases revealed by ovinised murine models Subject: Scientific Reports, doi:10.1038/s41598-020-61977-1 Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Thu Mar 12 06:16:44 2020 CET ModDate: Fri Mar 13 15:23:21 2020 CET Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 4419939 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SVABHT+Corbel-Bold CID TrueType Identity-H yes yes yes 115 0 MinionPro-Regular Type 1C Custom yes no yes 116 0 YRVKVS+Corbel-Bold2 Type 1C WinAnsi yes yes yes 117 0 PCHHPA+Corbel2 Type 1C WinAnsi yes yes yes 119 0 EXDATV+Corbel CID TrueType Identity-H yes yes yes 120 0 MyriadPro-It Type 1C WinAnsi yes no yes 121 0 MinionPro-It Type 1C Custom yes no yes 118 0 MinionPro-Bold Type 1C Custom yes no yes 137 0 EuclidSymbol Type 1C Custom yes no yes 153 0 WNWYGL+STIXGeneral-Regular Type 1C Custom yes yes yes 372 0 SHTACT+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 385 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-11-18 06:51:33 CET RepresentationInformation: pmc_32193445.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-06 03:55:01 CEST Size: 4419939 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd: Table 1. Clinical stage, clinical signs, genotype, date and age at sacrifice and flock of origin of sheep included in the study, and references of the inocula prepared from their tissues.:81' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 1. Western blot of inocula sourced from sheep tissues. (A) Inocula from medulla oblongata; (B) inocula from mesenteric lymph node. Note that all but one samples had banding patterns with the non-glycosylated (NG) band at 19⁜琀欀䐀愀⸀⃾＀䷾＀㨀 㨀㠀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈲ㄮ㤲㌠㈰㐮㘰ㄠ㈳㈮㐳㤠ㄹ㈮㐶崯䠯丯䅐㰼⽎‹㠠〠刾㸾㸊敮摯扪਴㠠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾＀￾＀㐀㘀⻾￾＀￾￾￾￾＀䠀漀瀀攀Ⰰ 䨀⸀⃾＀攀琀 愀泾＀⸀ 䴀漀氀攀挀甀氀愀爀 愀渀愀氀礀猀椀猀 漀昀 漀瘀椀渀攀 瀀爀椀漀渀 瀀爀漀琀攀椀渀 椀搀攀渀琀椀昀椀攀猀 猀椀洀椀氀愀爀椀琀椀攀猀 戀攀琀眀攀攀渀 䈀匀䔀 愀渀搀 愀渀 攀砀瀀攀爀椀洀攀渀琀愀氀 椀猀漀氀愀琀攀 漀昀 渀愀琀甀爀愀氀 猀挀爀愀瀀椀攀Ⰰ 䌀䠀㄀㘀㐀㄀⸀⃾＀䨀⸀ 䜀攀渀⸀ 嘀椀爀漀氀⻾＀⃾＀㠀ヾ＀倀琀 ㄀Ⰰ ㄠጀ㐀 ㄀㤀㤀㤀⻾＀㨀㠀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐷ㄮ㜱′〴⸶〱‴㜷⸳ㄲ‱㤲⸴㙝⽈⽎⽁值㰯丠㤹‰⁒㸾㸾੥湤潢樊㐹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 47. Baron, T. & Biacabe, A. G. Molecular behaviors of “CH1641-like” sheep scrapie isolates in ovine transgenic mice (TgOvPrP4). J. Virol. 81, 7230–7237 (2007). :84' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 1. Western blot of inocula sourced from sheep tissues. (A) Inocula from medulla oblongata; (B) inocula from mesenteric lymph node. Note that all but one samples had banding patterns with the non-glycosylated (NG) band at 19⁜琀欀䐀愀⸀⃾＀䷾＀㨀㨀㠀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐷㔮〳㈠ㄷ㐮㘰ㄠ㐸㔮㔳㘠ㄶ㈮㐶崯䠯丯䅐㰼⽎‱〳‰⁒㸾㸾੥湤潢樊㔳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 1. Western blot of inocula sourced from sheep tissues. (A) Inocula from medulla oblongata; (B) inocula from mesenteric lymph node. Note that all but one samples had banding patterns with the non-glycosylated (NG) band at 19⁜琀欀䐀愀⸀⃾＀䷾＀㨀㠀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈵㐮〱‱㐴⸶〱′㘳⸸㌹‱㌲⸴㙝⽈⽎⽁值㰯丠㄰㐠〠刾㸾㸊敮摯扪ਵ㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾＀￾＀㌀㌀⻾￾＀￾￾￾￾＀䈀攀爀椀渀最甀攀Ⰰ 嘀⸀Ⰰ 嘀椀氀漀琀琀攀Ⰰ 䨀⸀ 䰀⸀ ☀ 䰀愀甀搀攀Ⰰ 䠀⸀ 倀爀椀漀渀 愀最攀渀琀 搀椀瘀攀爀猀椀琀礀 愀渀搀 猀瀀攀挀椀攀猀 戀愀爀爀椀攀爀⸀⃾＀嘀攀琀⸀ 刀攀猀⻾＀⃾＀㌀㧾＀Ⰰ 㐀㜀 ㈀　　㠀⻾＀㨀㠀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷㄮ㔴ㄠ㄰㐮㘰ㄠㄷ㜮ㄵ㜠㤲⸴㙝⽈⽎⽁值㰯丠㄰㔠〠刾㸾㸊敮摯扪ਵ㔠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾＀￾＀㐀㘀⻾￾＀￾￾￾￾＀䠀漀瀀攀Ⰰ 䨀⸀⃾＀攀琀 愀泾＀⸀ 䴀漀氀攀挀甀氀愀爀 愀渀愀氀礀猀椀猀 漀昀 漀瘀椀渀攀 瀀爀椀漀渀 瀀爀漀琀攀椀渀 椀搀攀渀琀椀昀椀攀猀 猀椀洀椀氀愀爀椀琀椀攀猀 戀攀琀眀攀攀渀 䈀匀䔀 愀渀搀 愀渀 攀砀瀀攀爀椀洀攀渀琀愀氀 椀猀漀氀愀琀攀 漀昀 渀愀琀甀爀愀氀 猀挀爀愀瀀椀攀Ⰰ 䌀䠀㄀㘀㐀㄀⸀⃾＀䨀⸀ 䜀攀渀⸀ 嘀椀爀漀氀⻾＀⃾＀㠀ヾ＀倀琀 ㄀Ⰰ ㄠጀ㐀 ㄀㤀㤀㤀⻾＀㨀㤀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㠮㐹‱〴⸶〱‱㠴⸱〶‹㈮㐶崯䠯丯䅐㰼⽎‱〶‰⁒㸾㸾੥湤潢樊㔶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 50. Hill, A. F. et al. Molecular screening of sheep for bovine spongiform encephalopathy. Neurosci. Lett. 255, 159–162 (1998). :91' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 1. Western blot of inocula sourced from sheep tissues. (A) Inocula from medulla oblongata; (B) inocula from mesenteric lymph node. Note that all but one samples had banding patterns with the non-glycosylated (NG) band at 19⁜琀欀䐀愀⸀⃾＀䷾＀㨀㠀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈵㐮〱‱㐴⸶〱′㘳⸸㌹‱㌲⸴㙝⽈⽎⽁值㰯丠㄰㐠〠刾㸾㸊敮摯扪ਵ㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾＀￾＀㌀㌀⻾￾＀￾￾￾￾＀䈀攀爀椀渀最甀攀Ⰰ 嘀⸀Ⰰ 嘀椀氀漀琀琀攀Ⰰ 䨀⸀ 䰀⸀ ☀ 䰀愀甀搀攀Ⰰ 䠀⸀ 倀爀椀漀渀 愀最攀渀琀 搀椀瘀攀爀猀椀琀礀 愀渀搀 猀瀀攀挀椀攀猀 戀愀爀爀椀攀爀⸀⃾＀嘀攀琀⸀ 刀攀猀⻾＀⃾＀㌀㧾＀Ⰰ 㐀㜀 ㈀　　㠀⻾＀㨀㠀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷㄮ㔴ㄠ㄰㐮㘰ㄠㄷ㜮ㄵ㜠㤲⸴㙝⽈⽎⽁值㰯丠㄰㔠〠刾㸾㸊敮摯扪ਵ㔠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾＀￾＀㐀㘀⻾￾＀￾￾￾￾＀䠀漀瀀攀Ⰰ 䨀⸀⃾＀攀琀 愀泾＀⸀ 䴀漀氀攀挀甀氀愀爀 愀渀愀氀礀猀椀猀 漀昀 漀瘀椀渀攀 瀀爀椀漀渀 瀀爀漀琀攀椀渀 椀搀攀渀琀椀昀椀攀猀 猀椀洀椀氀愀爀椀琀椀攀猀 戀攀琀眀攀攀渀 䈀匀䔀 愀渀搀 愀渀 攀砀瀀攀爀椀洀攀渀琀愀氀 椀猀漀氀愀琀攀 漀昀 渀愀琀甀爀愀氀 猀挀爀愀瀀椀攀Ⰰ 䌀䠀㄀㘀㐀㄀⸀⃾＀䨀⸀ 䜀攀渀⸀ 嘀椀爀漀氀⻾＀⃾＀㠀ヾ＀倀琀 ㄀Ⰰ ㄠጀ㐀 ㄀㤀㤀㤀⻾＀㨀㤀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㠮㐹‱〴⸶〱‱㠴⸱〶‹㈮㐶崯䠯丯䅐㰼⽎‱〶‰⁒㸾㸾੥湤潢樊㔶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 50. Hill, A. F. et al. Molecular screening of sheep for bovine spongiform encephalopathy. Neurosci. Lett. 255, 159–162 (1998). :91' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd: Table 2. Banding patterns of source sheep tissues and survival periods, attack rates and banding patterns of experimentally challenged first and second-passage TgShp XI and Tg338 mice. Survival period provided as:96' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 2. Western blot of spinal cord homogenates from second-passage TgShp XI mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that most samples had banding patterns with t:97' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 3. Western blot of spinal cord homogenates from second-passage Tg338 mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that 13/18 samples were clearly positive with no:98' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 3. Western blot of spinal cord homogenates from second-passage Tg338 mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that 13/18 samples were clearly positive with n:99' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 2. Western blot of spinal cord homogenates from second-passage TgShp XI mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that most samples had banding patterns with :100' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 2. Western blot of spinal cord homogenates from second-passage TgShp XI mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that most samples had banding patterns with:101' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 2. Western blot of spinal cord homogenates from second-passage TgShp XI mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that most samples had banding patterns wit:102' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 1. Western blot of inocula sourced from sheep tissues. (A) Inocula from medulla oblongata; (B) inocula from mesenteric lymph node. Note that all but one samples had banding patterns with the non-glycosylated (NG) band at 19⁜琀欀䐀愀⸀⃾＀䴀㨀㄀　㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌶㈮ㄸ㜠㈵㜮㈶㌠㌷ㄮ㠷㜠㈴㔮ㄲ㉝⽈⽎⽁值㰯丠ㄷ㐠〠刾㸾㸊敮摯扪਱㘶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 3. Western blot of spinal cord homogenates from second-passage Tg338 mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that 13/18 samples were clearly positive with :104' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 3. Western blot of spinal cord homogenates from second-passage Tg338 mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that 13/18 samples were clearly positive with :104' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 3. Western blot of spinal cord homogenates from second-passage Tg338 mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that 13/18 samples were clearly positive with:105' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 3. Western blot of spinal cord homogenates from second-passage Tg338 mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Note that 13/18 samples were clearly positive wit:106' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 4. Lesion profiles of second-passage TgShp XI mice. Both inocula associated to the 19K phenotype (A) and inocula associated to the 21K-TgShp XI phenotype (B) provoked similarly shaped lesion profiles in TgShp XI mice (C), most of th:107' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 4. Lesion profiles of second-passage TgShp XI mice. Both inocula associated to the 19K phenotype (A) and inocula associated to the 21K-TgShp XI phenotype (B) provoked similarly shaped lesion profiles in TgShp XI mice (C), most of t:108' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 4. Lesion profiles of second-passage TgShp XI mice. Both inocula associated to the 19K phenotype (A) and inocula associated to the 21K-TgShp XI phenotype (B) provoked similarly shaped lesion profiles in TgShp XI mice (C), most of :109' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 5. Lesion profiles of second-passage Tg338 mice. Inocula associated to the 19K phenotype provoked similar lesion profiles in Tg338 mice, mostly characterized by peaks at medulla oblongata and thalamus (A). However, isolates linked to th:110' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 5. Lesion profiles of second-passage Tg338 mice. Inocula associated to the 19K phenotype provoked similar lesion profiles in Tg338 mice, mostly characterized by peaks at medulla oblongata and thalamus (A). However, isolates linked to :112' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 6. PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2⁜琀一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀ⴀ椀渀㨀㄀㄀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌳㈮㈱㈠㔰㐮㘸㈠㌵㠮㐸ㄠ㐹㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㈠〠刾㸾㸊敮摯扪਱㤴‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 6. PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2⁜琀一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀ⴀ椀㨀㄀㄀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔰〮ㄹ㘠㐷㐮㘸㈠㔱㠮㌳㈠㐶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㌠〠刾㸾㸊敮摯扪਱㤵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 6. PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2⁜琀一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀ⴀ㨀㄀㄀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌶〮㘹‴㘴⸶㠲″㜱⸶㔹‴㔲⸵㐱崯䠯丯䅐㰼⽎′㈴‰⁒㸾㸾੥湤潢樊ㄹ㘠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾￾￾＀䘀椀最甀爀攀 㛾￾＀⻾￾＠˾￾￾＀倀爀僾＀匀揾＀ 搀椀猀琀爀椀戀甀琀椀漀渀 椀渀 爀攀瀀爀攀猀攀渀琀愀琀椀瘀攀 戀爀愀椀渀 猀攀挀琀椀漀渀猀 昀爀漀洀 吀最匀栀瀀 堀䤀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 ㈠一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀㨀㄀㄀㘩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈳㈮㌶㌠㐵㐮㘸㈠㈴ㄮ㜹㔠㐴㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㔠〠刾㸾㸊敮摯扪਱㤷‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀椀㨀㄀㄀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㈮㈷㔠㐱㐮㘸㈠㈸㘮㘸㠠㐰㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㘠〠刾㸾㸊敮摯扪਱㤸‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀㨀㄀㄀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㐮㠶㤠㌷㐮㜹㤠㈰〮㌳㤠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㜠〠刾㸾㸊敮摯扪਱㤹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀㨀㄀㄀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌰㐮㜶㜠㌷㐮㜹㤠㌲㌮㜲㘠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㠠〠刾㸾㸊敮摯扪ਲ〰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 6. PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2⁜琀一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀ⴀ椀㨀㄀㄀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔰〮ㄹ㘠㐷㐮㘸㈠㔱㠮㌳㈠㐶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㌠〠刾㸾㸊敮摯扪਱㤵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 6. PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2⁜琀一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀ⴀ㨀㄀㄀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌶〮㘹‴㘴⸶㠲″㜱⸶㔹‴㔲⸵㐱崯䠯丯䅐㰼⽎′㈴‰⁒㸾㸾੥湤潢樊ㄹ㘠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾￾￾＀䘀椀最甀爀攀 㛾￾＀⻾￾＠˾￾￾＀倀爀僾＀匀揾＀ 搀椀猀琀爀椀戀甀琀椀漀渀 椀渀 爀攀瀀爀攀猀攀渀琀愀琀椀瘀攀 戀爀愀椀渀 猀攀挀琀椀漀渀猀 昀爀漀洀 吀最匀栀瀀 堀䤀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 ㈠一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀㨀㄀㄀㘩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈳㈮㌶㌠㐵㐮㘸㈠㈴ㄮ㜹㔠㐴㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㔠〠刾㸾㸊敮摯扪਱㤷‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀椀㨀㄀㄀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㈮㈷㔠㐱㐮㘸㈠㈸㘮㘸㠠㐰㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㘠〠刾㸾㸊敮摯扪਱㤸‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀㨀㄀㄀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㐮㠶㤠㌷㐮㜹㤠㈰〮㌳㤠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㜠〠刾㸾㸊敮摯扪਱㤹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀㨀㄀㄀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌰㐮㜶㜠㌷㐮㜹㤠㌲㌮㜲㘠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㠠〠刾㸾㸊敮摯扪ਲ〰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 6. PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2⁜琀一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀ⴀ㨀㄀㄀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌶〮㘹‴㘴⸶㠲″㜱⸶㔹‴㔲⸵㐱崯䠯丯䅐㰼⽎′㈴‰⁒㸾㸾੥湤潢樊ㄹ㘠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾￾￾＀䘀椀最甀爀攀 㛾￾＀⻾￾＠˾￾￾＀倀爀僾＀匀揾＀ 搀椀猀琀爀椀戀甀琀椀漀渀 椀渀 爀攀瀀爀攀猀攀渀琀愀琀椀瘀攀 戀爀愀椀渀 猀攀挀琀椀漀渀猀 昀爀漀洀 吀最匀栀瀀 堀䤀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 ㈠一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀㨀㄀㄀㘩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈳㈮㌶㌠㐵㐮㘸㈠㈴ㄮ㜹㔠㐴㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㔠〠刾㸾㸊敮摯扪਱㤷‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀椀㨀㄀㄀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㈮㈷㔠㐱㐮㘸㈠㈸㘮㘸㠠㐰㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㘠〠刾㸾㸊敮摯扪਱㤸‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀㨀㄀㄀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㐮㠶㤠㌷㐮㜹㤠㈰〮㌳㤠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㜠〠刾㸾㸊敮摯扪਱㤹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀㨀㄀㄀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌰㐮㜶㜠㌷㐮㜹㤠㌲㌮㜲㘠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㠠〠刾㸾㸊敮摯扪ਲ〰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 6. PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2⁜琀一 ＀䇾＀Ⰰ ㌠一 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⸀ 吀眀漀 搀椀昀昀攀爀攀渀琀 琀爀攀渀搀猀 漀昀 倀爀僾＀匀揾＀ 愀挀挀甀洀甀氀愀琀椀漀渀 眀攀爀攀 漀戀猀攀爀瘀攀搀 漀渀 倀䔀吀ⴀ戀氀漀琀猀㨀 愀 最攀渀攀爀愀氀椀稀攀搀 氀漀眀㨀㄀㄀㘩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈳㈮㌶㌠㐵㐮㘸㈠㈴ㄮ㜹㔠㐴㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㔠〠刾㸾㸊敮摯扪਱㤷‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀椀㨀㄀㄀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㈮㈷㔠㐱㐮㘸㈠㈸㘮㘸㠠㐰㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㘠〠刾㸾㸊敮摯扪਱㤸‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀㨀㄀㄀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㐮㠶㤠㌷㐮㜹㤠㈰〮㌳㤠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㜠〠刾㸾㸊敮摯扪਱㤹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀㨀㄀㄀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌰㐮㜶㜠㌷㐮㜹㤠㌲㌮㜲㘠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㠠〠刾㸾㸊敮摯扪ਲ〰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀椀㨀㄀㄀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㈮㈷㔠㐱㐮㘸㈠㈸㘮㘸㠠㐰㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㘠〠刾㸾㸊敮摯扪਱㤸‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀㨀㄀㄀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㐮㠶㤠㌷㐮㜹㤠㈰〮㌳㤠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㜠〠刾㸾㸊敮摯扪਱㤹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀㨀㄀㄀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌰㐮㜶㜠㌷㐮㜹㤠㌲㌮㜲㘠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㠠〠刾㸾㸊敮摯扪ਲ〰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀愀㨀㄀㄀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛ㄷ㐮㠶㤠㌷㐮㜹㤠㈰〮㌳㤠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㜠〠刾㸾㸊敮摯扪਱㤹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀㨀㄀㄀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌰㐮㜶㜠㌷㐮㜹㤠㌲㌮㜲㘠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㠠〠刾㸾㸊敮摯扪ਲ〰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀爀㨀㄀㄀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌰㐮㜶㜠㌷㐮㜹㤠㌲㌮㜲㘠㌶㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㠠〠刾㸾㸊敮摯扪ਲ〰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 戀㨀㄀㈀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㐮㈱㐠㌶㐮㘸㈠㈳ㄮ〹㈠㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈲㤠〠刾㸾㸊敮摯扪ਲ〱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀 㨀㄀㈀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐸㈮㘷㜠㌶㐮㘸㈠㔰〮㘷ㄠ㌵㈮㔴ㅝ⽈⽎⽁值㰯丠㈳〠〠刾㸾㸊敮摯扪ਲ〲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 7. PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1⁜琀䰀 ＀䇾＀Ⰰ ㌠䰀 ＀䋾＀Ⰰ 㜠䰀 ＀䏾＀ 愀渀搀 㠠䰀 ＀䓾＀⳾＀䗾＀⸀ 䄀 最攀渀攀爀愀氀 瀀愀琀琀攀爀渀 愀爀漀猀攀 椀渀 愀氀氀 攀砀愀洀椀渀攀搀 戀爀愀椀渀 猀愀洀瀀氀攀猀 挀漀渀猀椀猀琀椀渀最 漀昀 椀渀琀攀渀猀攀 猀琀愀椀渀椀渀最 漀昀㨀㄀㈀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈶㐮㜴″ㄴ⸶㠲′㠲⸴㠠㌰㈮㔴ㅝ⽈⽎⽁值㰯丠㈳ㄠ〠刾㸾㸊敮摯扪ਲ〳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 眀㨀㄀㈀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐹㜮㔵″ㄴ⸶㠲‵㈹⸸㤸″〲⸵㐱崯䠯丯䅐㰼⽎′㌲‰⁒㸾㸾੥湤潢樊㈰㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㘀㄀㤀㜀㜀⸀椀渀搀搀㫾￾￾￾＀䘀椀最甀爀攀 㣾￾＀⻾￾＠˾￾￾＀唀渀椀挀攀渀琀爀椀挀 ＀䇾＀ 琀漀⃾＀䏾＀Ⰰ 昀氀漀爀椀搀 ＀䓾＀ 琀漀⃾＀䛾＀ 愀渀搀 愀洀漀爀瀀栀漀甀猀 瀀攀爀椀瘀愀猀挀甀氀愀爀 瀀氀愀焀甀攀猀 ＀䟾＀ 琀漀⃾＀䳾＀ 椀渀 戀爀愀椀渀 漀昀 猀攀挀漀渀搀ⴀ瀀愀猀猀愀最攀 吀最㌀㌀㠀 洀椀挀攀 椀渀昀攀挀琀攀搀 眀椀琀栀 椀渀漀挀甀氀愀 㘠䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀 㨀㄀㈀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌸ㄮ㤴㘠㌰㐮㘸㈠㐰㤮㠶㜠㈹㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㌠〠刾㸾㸊敮摯扪ਲ〵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd:Figure 8. Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6⁜琀䰀 愀渀搀 㠠䰀⸀ 䠀愀攀洀愀琀漀砀椀氀椀渀ⴀ攀漀猀椀渀 猀琀愀椀渀椀渀最 ＀䇾＀⳾＀䓾＀⳾＀䟾＀⳾＀䫾＀Ⰰ 椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀㨀㄀㈀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈸㌮㠵㠠㈹㐮㘸㈠㌱㜮㔳㘠㈸㈮㔴ㅝ⽈⽎⽁值㰯丠㈳㐠〠刾㸾㸊敮摯扪ਲ〶‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_61977.indd: 52. Jeffrey, M., Martin, S. & Gonzalez, L. Cell-associated variants of disease-specific prion protein immunolabelling are found in different sources of sheep transmissible spongiform encephalopathy. J. Gen. Virol. 84, 1033–1045 (2003). :126' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd: 26. Bruce, M. E. et al. Strain characterization of natural sheep scrapie and comparison with BSE. J. Gen. Virol. 83, 695–704 (2002). :129' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd: 47. Baron, T. & Biacabe, A. G. Molecular behaviors of “CH1641-like” sheep scrapie isolates in ovine transgenic mice (TgOvPrP4). J. Virol. 81, 7230–7237 (2007). :144' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd: 46. Hope, J. et al. Molecular analysis of ovine prion protein identifies similarities between BSE and an experimental isolate of natural scrapie, CH1641. J. Gen. Virol. 80(Pt 1), 1–4 (1999). :145' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd: 49. Cassard, H. et al. Evidence for zoonotic potential of ovine scrapie prions. Nat. Commun. 5, 5821 (2014). :146' cannot be found Offset: 4091372 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_61977.indd: 25. Collinge, J. & Clarke, A. R. A general model of prion strains and their pathogenicity. Science 318, 930–936 (2007). :149' cannot be found Offset: 4091372 MIMEtype: application/pdf PDFMetadata: Objects: 598 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Mixtures of prion substrains in natural scrapie cases revealed by ovinised murine models Children: Item: Title: Results Children: Item: Title: Biochemical characterization of nervous and lymphoid tissue sheep scrapie field isolates discloses different biochemical pa ... Item: Title: Bioassay in sheep PrPC-expressing transgenic mice suggest the presence of more than one prion variant. Item: Title: Mice brains show distinct histopathological hallmarks. Item: Title: Discussion Item: Title: Methods Children: Item: Title: Sheep. Item: Title: Inocula. Item: Title: Mice. Item: Title: Intracerebral inoculation. Item: Title: Bioassay. Item: Title: Western blotting. Item: Title: Tissue processing. Item: Title: Haematoxylin and eosin staining. Item: Title: Immunohistochemistry. Item: Title: Paraffin-embedded tissue-blot (PET-blot). Item: Title: Lesion and PrPSc distribution profiling. Item: Title: Acknowledgements Item: Title: Figure 1 Western blot of inocula sourced from sheep tissues. Item: Title: Figure 2 Western blot of spinal cord homogenates from second-passage TgShp XI mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Item: Title: Figure 3 Western blot of spinal cord homogenates from second-passage Tg338 mice experimentally challenged with (A) sheep brain-derived isolates and (B) sheep lymph node-derived isolates. Item: Title: Figure 4 Lesion profiles of second-passage TgShp XI mice. Item: Title: Figure 5 Lesion profiles of second-passage Tg338 mice. Item: Title: Figure 6 PrPSc distribution in representative brain sections from TgShp XI mice infected with inocula 2 N (A), 3 N (B), 7 L (C) and 8 L (D). Item: Title: Figure 7 PrPSc distribution in representative brain sections from Tg338 mice infected with inocula 1 L (A), 3 L (B), 7 L (C) and 8 L (D,E). Item: Title: Figure 8 Unicentric (A to C), florid (D to F) and amorphous perivascular plaques (G to L) in brain of second-passage Tg338 mice infected with inocula 6 L and 8 L. Item: Title: Table 1 Clinical stage, clinical signs, genotype, date and age at sacrifice and flock of origin of sheep included in the study, and references of the inocula prepared from their tissues. Item: Title: Table 2 Banding patterns of source sheep tissues and survival periods, attack rates and banding patterns of experimentally challenged first and second-passage TgShp XI and Tg338 mice. 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