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Linearized PDF PDFMetadata: Objects: 1065 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Proteasomal-Mediated Degradation of AKAP150 Accompanies AMPAR Endocytosis during cLTD Destination: bkmk_bookmark_1 Children: Item: Title: Introduction Destination: bkmk_bookmark_2 Item: Title: Materials and Methods Destination: bkmk_bookmark_3 Children: Item: Title: Cell culture, transfection, and transduction Destination: bkmk_bookmark_4 Item: Title: Plasmids and lentivirus production Destination: bkmk_bookmark_5 Item: Title: Cell stimulation and lysate preparation Destination: bkmk_bookmark_6 Item: Title: Surface biotinylation Destination: bkmk_bookmark_7 Item: Title: Immunoprecipitation Destination: bkmk_bookmark_8 Item: Title: Immunoblotting Destination: bkmk_bookmark_9 Item: Title: Immunocytochemistry Destination: bkmk_bookmark_10 Item: Title: Confocal imaging and analysis Destination: bkmk_bookmark_11 Item: Title: Statistical analysis Destination: bkmk_bookmark_12 Item: Title: Results Destination: bkmk_bookmark_13 Children: Item: Title: cLTD triggers a decrease in Ser-845 GluA1 phosphorylation, GluA1 protein levels, GluA1-AMPAR surface expression, and AKAP150 degradation Destination: bkmk_bookmark_14 Item: Title: Ubiquitin-proteasome system mediates cLTD-dependent AKAP150 degradation Destination: bkmk_bookmark_15 Item: Title: Endogenous AKAP150 knock-down triggers dephosphorylation and endocytosis of AMPARs Destination: bkmk_bookmark_16 Item: Title: Overexpression of AKAP150 blocks cLTD-mediated AMPARs endocytosis Destination: bkmk_bookmark_17 Item: Title: Discussion Destination: bkmk_bookmark_18 Item: Title: References Destination: bkmk_bookmark_19 Info: Title: SN-ENUJ200072 1..19 Creator: Arbortext Advanced Print Publisher 11.0.3352/W Unicode Producer: Adobe LiveCycle PDFG ES CreationDate: Tue Mar 31 12:51:48 CEST 2020 ModDate: Tue Mar 31 12:51:48 CEST 2020 ID: 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