4517f8ed3d62b78a64e75e23f8f6c100 pmc_32230770.pdf bed638182656b91b4280d4aa749dc7fd01406214 pmc_32230770.pdf ab4195a3da3fecffe0d3d8c49e802fc8c44d7cede6056ffb5669f2dd64049796 pmc_32230770.pdf Title: NeuroHeal Treatment Alleviates Neuropathic Pain and Enhances Sensory Axon Regeneration Subject: Peripheral nerve injury (PNI) leads to the loss of motor, sensory, and autonomic functions, and often triggers neuropathic pain. During the last years, many efforts have focused on finding new therapies to increase axonal regeneration or to alleviate painful conditions. Still only a few of them have targeted both phenomena. Incipient or aberrant sensory axon regeneration is related to abnormal unpleasant sensations, such as hyperalgesia or allodynia. We recently have discovered NeuroHeal, a combination of two repurposed drugs; Acamprosate and Ribavirin. NeuroHeal is a neuroprotective agent that also enhances motor axon regeneration after PNI. In this work, we investigated its effect on sensory fiber regeneration and PNI-induced painful sensations in a rat model of spare nerve injury and nerve crush. The follow up of the animals showed that NeuroHeal treatment reduced the signs of neuropathic pain in both models. Besides, the treatment favored sensory axon regeneration, as observed in dorsal root ganglion explants. Mechanistically, the effects observed in vivo may improve the resolution of cell-protective autophagy. Additionally, NeuroHeal treatment modulated the P2X4-BDNF-KCC2 axis, which is an essential driver of neuropathic pain. These data open a new therapeutic avenue based on autophagic modulation to foster endogenous regenerative mechanisms and reduce the appearance of neuropathic pain in PNI. Keywords: peripheral nerve injury; neuropathic pain; nerve regeneration; autophagy; NeuroHeal Author: David Romeo-Guitart and Caty Casas Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Tue Mar 31 09:46:39 2020 CEST ModDate: Tue Mar 31 09:46:39 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2784105 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PZNNWZ+URWPalladioL-Ital Type 1 Custom yes yes no 119 0 MGYHKW+URWPalladioL-Bold Type 1 Custom yes yes no 120 0 VSJHNQ+URWPalladioL-Roma Type 1 Custom yes yes no 121 0 KVFQPQ+VnURWPalladioL Type 1 Custom yes yes no 122 0 FATHTC+Rpxr Type 1 Builtin yes yes no 123 0 PFKOHU+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 128 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes 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Type 1 Builtin yes yes no 541 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 600 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:44:50 CEST RepresentationInformation: pmc_32230770.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 11:44:23 CEST Size: 2784105 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰ぃふぬぴふひづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぁへはのちぬぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰いひふでびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぁのどねちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰こふひでづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぅぬづっぴひはばとべびどはぬはでどっちぬぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰ぐひはっづびびどのでぜ〴ぜ〰てはひぜ〴ぜ〰えどびぴはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰ぃふぬぴふひづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぁへはのちぬぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰いひふでびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぁのどねちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰こふひでづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぅぬづっぴひはばとべびどはぬはでどっちぬぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰ぐひはっづびびどのでぜ〴ぜ〰てはひぜ〴ぜ〰えどびぴはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: いひふでびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぁのどねちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰こふひでづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぅぬづっぴひはばとべびどはぬはでどっちぬぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰ぐひはっづびびどのでぜ〴ぜ〰てはひぜ〴ぜ〰えどびぴはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぁのどねちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰こふひでづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぅぬづっぴひはばとべびどはぬはでどっちぬぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰ぐひはっづびびどのでぜ〴ぜ〰てはひぜ〴ぜ〰えどびぴはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぅぬづっぴひはばとべびどはぬはでどっちぬぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰ぐひはっづびびどのでぜ〴ぜ〰てはひぜ〴ぜ〰えどびぴはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぅぬづっぴひはばとべびどはぬはでどっちぬぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰ぐひはっづびびどのでぜ〴ぜ〰てはひぜ〴ぜ〰えどびぴはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ごどびびふづぜ〴ぜ〰ぐひはっづびびどのでぜ〴ぜ〰てはひぜ〴ぜ〰えどびぴはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰ちのつぜ〴ぜ〰ぉねちでづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㤩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰ぅのとちのっづびぜ〴ぜ〰げづでづのづひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰こづのびはひべぜ〴ぜ〰ちのつぜ〴ぜ〰きはぴはひぜ〴ぜ〰ぁへはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰げづつふっづびぜ〴ぜ〰えべばづひちぬでづびどちぜ〴ぜ〰ちてぴづひぜ〴ぜ〰ぐづひどばとづひちぬぜ〴ぜ〰ぎづひぶづぜ〴ぜ〰ぉのなふひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰げづつふっづびぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁふぴはばとちでべぜ〴ぜ〰ぉねばちどひねづのぴぜ〴ぜ〰どびぜ〴ぜ〰げづびはぬぶづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぎづふひはえづちぬぜ〴ぜ〰ごひづちぴねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰ぉのっひづちびづびぜ〴ぜ〰かぃぃ〲ぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰いはひびちぬぜ〴ぜ〰こばどのちぬぜ〴ぜ〰えはひのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ぎづふひはえづちぬぜ〴ぜ〰ぅのとちのっづびぜ〴ぜ〰げづでづのづひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰こづのびはひべぜ〴ぜ〰ちのつぜ〴ぜ〰きはぴはひぜ〴ぜ〰ぁへはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰げづつふっづびぜ〴ぜ〰えべばづひちぬでづびどちぜ〴ぜ〰ちてぴづひぜ〴ぜ〰ぐづひどばとづひちぬぜ〴ぜ〰ぎづひぶづぜ〴ぜ〰ぉのなふひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰げづつふっづびぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁふぴはばとちでべぜ〴ぜ〰ぉねばちどひねづのぴぜ〴ぜ〰どびぜ〴ぜ〰げづびはぬぶづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぎづふひはえづちぬぜ〴ぜ〰ごひづちぴねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰ぉのっひづちびづびぜ〴ぜ〰かぃぃ〲ぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰いはひびちぬぜ〴ぜ〰こばどのちぬぜ〴ぜ〰えはひのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぎづふひはえづちぬぜ〴ぜ〰げづつふっづびぜ〴ぜ〰えべばづひちぬでづびどちぜ〴ぜ〰ちてぴづひぜ〴ぜ〰ぐづひどばとづひちぬぜ〴ぜ〰ぎづひぶづぜ〴ぜ〰ぉのなふひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰げづつふっづびぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁふぴはばとちでべぜ〴ぜ〰ぉねばちどひねづのぴぜ〴ぜ〰どびぜ〴ぜ〰げづびはぬぶづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぎづふひはえづちぬぜ〴ぜ〰ごひづちぴねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰ぉのっひづちびづびぜ〴ぜ〰かぃぃ〲ぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰いはひびちぬぜ〴ぜ〰こばどのちぬぜ〴ぜ〰えはひのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぎづふひはえづちぬぜ〴ぜ〰げづつふっづびぜ〴ぜ〰きづっとちのどっちぬぜ〴ぜ〰ぁぬぬはつべのどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁふぴはばとちでべぜ〴ぜ〰ぉねばちどひねづのぴぜ〴ぜ〰どびぜ〴ぜ〰げづびはぬぶづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぎづふひはえづちぬぜ〴ぜ〰ごひづちぴねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰ぉのっひづちびづびぜ〴ぜ〰かぃぃ〲ぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰いはひびちぬぜ〴ぜ〰こばどのちぬぜ〴ぜ〰えはひのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぁふぴはばとちでべぜ〴ぜ〰ぉねばちどひねづのぴぜ〴ぜ〰どびぜ〴ぜ〰げづびはぬぶづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぎづふひはえづちぬぜ〴ぜ〰ごひづちぴねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぎづふひはえづちぬぜ〴ぜ〰ぉのっひづちびづびぜ〴ぜ〰かぃぃ〲ぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰いはひびちぬぜ〴ぜ〰こばどのちぬぜ〴ぜ〰えはひのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぎづふひはえづちぬぜ〴ぜ〰ぉのっひづちびづびぜ〴ぜ〰かぃぃ〲ぜ〴ぜ〰がづぶづぬびぜ〴ぜ〰どのぜ〴ぜ〰いはひびちぬぜ〴ぜ〰こばどのちぬぜ〴ぜ〰えはひのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: こぴふつべぜ〴ぜ〰がどねどぴちぴどはのびぜ〴ぜ〰ちのつぜ〴ぜ〰うふぴふひづぜ〴ぜ〰いどひづっぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸶ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸷ Item: Title: ぐちぴづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸷ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: KNZVOU+Rpxsc FontSubset: true FirstChar: 100 LastChar: 100 FontDescriptor: FontName: KNZVOU+Rpxsc Flags: Symbolic FontBBox: -37, -172, 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