9704380e2c888ab840e9298856f50885 pmc_32244308.pdf 90428c55b455badf72f67436f21ef8460b9235c8 pmc_32244308.pdf 50af44277b0f059651d4ed6ded9cafc5eefe6444181cd4a984f82538948bcfe5 pmc_32244308.pdf Producer: iText® 7.1.1 ©2000-2018 iText Group NV (AGPL-version) CreationDate: Thu Apr 9 03:29:37 2020 CEST ModDate: Thu Apr 9 03:29:37 2020 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4181802 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- VBANAT+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 TDUHYL+VnURWPalladioL Type 1 Custom yes yes no 15 0 UOXJSA+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 OZFIXI+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 SFXJBC+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 SYXPPI+Rpxr Type 1 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Children: Item: Title: Cloning of Human Recombinant sIFNAR2 Destination: subsection.2.1 Item: Title: Purification of the Recombinant sIFNAR2 Protein Destination: subsection.2.2 Item: Title: Human Samples Destination: subsection.2.3 Item: Title: Cell Cultures in the Presence of Recombinant sIFNAR2 Destination: subsection.2.4 Item: Title: Determination of Cytokines in Supernatant by Luminex Destination: subsection.2.5 Item: Title: Determination of Intracellular Cytokines byFlow Cytometry Destination: subsection.2.6 Item: Title: Analysis of Cell Proliferation Destination: subsection.2.7 Item: Title: Cytopathic Effect Test (CPE) or Bioassay A549/EMCV Destination: subsection.2.8 Item: Title: Determination of p-STAT1 by Flow Cytometry after IFN and Recombinant sIFNAR2 Stimulation Destination: subsection.2.9 Item: Title: MxA Expression by Real Time PCR after IFN and Recombinant sIFNAR2 Stimulation Destination: subsection.2.10 Item: Title: RNA-Seq and Differential Expression Analyses Destination: subsection.2.11 Item: Title: Data Analysis Destination: subsection.2.12 Item: Title: Results Destination: section.3 Children: Item: Title: Effect of Recombinant sIFNAR2 on the Cytokine Expression Pattern in Human Cells Destination: subsection.3.1 Children: Item: Title: Production of Secreted Cytokines, Chemokines and Growth Factors Destination: subsubsection.3.1.1 Item: Title: Production of Intracellular Cytokines Destination: subsubsection.3.1.2 Item: Title: Antiproliferative Activity of Recombinant sIFNAR2 Destination: subsection.3.2 Item: Title: Antiviral Activity of Recombinant sIFNAR2 by CPE (Cytopathic Effect) Inhibition Assay (A549/EMCV) Destination: subsection.3.3 Children: Item: Title: Experimental Conditions Tested to Demonstrate the Specific Antiviral Activity of the Recombinant sIFNAR2. Destination: subsubsection.3.3.1 Item: Title: The Antiviral Activity of Recombinant sIFNAR2 is Independent of IFN-ß Destination: subsubsection.3.3.2 Item: Title: The Mechanism of Action of Human Recombinant sIFNAR2 is Independent of the JAK-STAT Signaling Pathway Destination: subsection.3.4 Children: Item: Title: Recombinant sIFNAR2 Stimulation does not Activate STAT1 in Human Cells Destination: subsubsection.3.4.1 Item: Title: Recombinant sIFNAR2 Stimulation does not Induce MxA in either Human PBMC or Jurkat Cells Destination: subsubsection.3.4.2 Item: Title: Recombinant sIFNAR2 Elicits a Different Gene Expression Response to IFN-ß Destination: subsubsection.3.4.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: Patents Destination: section.6 Item: Title: References Destination: section.6 Info: Producer: iText® 7.1.1 ©2000-2018 iText Group NV (AGPL-version) CreationDate: Thu Apr 09 03:29:37 CEST 2020 ModDate: Thu Apr 09 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