2b9e85dd33d4f8fe9dd97dcb4a0a01a1 pmc_32255384.pdf 78c35189edbb3b09137488f549b49fa04048dddb pmc_32255384.pdf a8cbb1d84666d0cb24a4a2601cf853a441e130edd8838babbb270d6cd5eccf6e pmc_32255384.pdf Title: Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978 Subject: Virulence, 2020. doi:10.1080/21505594.2020.1748923 Keywords: A. baumannii; surface-associated motility; chemotaxis; virulence; RecA; CheW-like protein Author: Jordi Corral Creator: Arbortext Advanced Print Publisher 11.0.3433/W Unicode Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Mon Apr 6 22:25:00 2020 CEST ModDate: Mon Apr 6 22:26:02 2020 CEST Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 12 Encrypted: no Page size: 593.972 x 792 pts Page rot: 0 File size: 3577191 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KJCJDL+AdvOT1ef757c0+20 Type 1C WinAnsi yes yes no 337 0 KJCJPH+AdvOT5fcf1b24+20 Type 1C WinAnsi yes yes no 339 0 KJCJAC+AdvOT46dcae81 Type 1C WinAnsi yes yes no 341 0 KJCJAD+AdvOTce3d9a73 Type 1C WinAnsi yes yes no 343 0 KJCJBE+AdvOT56309c18.I Type 1C WinAnsi yes yes no 345 0 KJCJBF+AdvOT5fcf1b24 Type 1C WinAnsi yes yes no 347 0 KJCJBG+AdvOT2bda31c3.B Type 1C WinAnsi yes yes no 349 0 KJCJBH+AdvOT65f8a23b.I Type 1C WinAnsi yes yes no 351 0 KJCJCH+AdvOT5c7b8c86.I Type 1C WinAnsi yes yes no 353 0 KJCJCI+AdvOT3b30f6db.B Type 1C WinAnsi yes yes no 355 0 KJCJDJ+AdvOT7d6df7ab.I Type 1C WinAnsi yes yes no 357 0 KJCJDK+AdvOT1ef757c0 Type 1C WinAnsi yes yes no 359 0 KJCKIE+AdvOTd0125be5.BI Type 1C WinAnsi yes yes no 240 0 KJCKIF+AdvOT1ef757c0+03 Type 1C Custom yes yes yes 237 0 KJCLAG+AdvOT5fcf1b24+22 Type 1C Custom yes yes no 249 0 KJCKPE+AdvOTc20ddc96 Type 1C WinAnsi yes yes no 250 0 KJCKPF+AdvOT5fcf1b24+03 Type 1C Custom yes yes yes 248 0 KJCKPG+AdvOT5c7b8c86.I+03 Type 1C Custom yes yes yes 244 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:12:48 CEST RepresentationInformation: pmc_32255384.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:44:25 CEST Size: 3577191 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: true NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: OneColumn PageMode: UseOutlines Language: English Outlines: Item: Title: Abstract Destination: 1 Item: Title: Introduction Destination: 1 Item: Title: Materials and methods Destination: 1 Children: Item: Title: Bacterial strains, plasmids and growth conditions Destination: 1 Item: Title: Accession numbers Destination: 1 Item: Title: A.baumannii knockout construction and complementation Destination: 1 Item: Title: Surface-associated motility assays Destination: 1 Item: Title: Chemotaxis assays Destination: 1 Item: Title: Transmission electron microscopy (TEM) Destination: 1 Item: Title: Nematode fertility assays Destination: 1 Item: Title: Galleria killing assays Destination: 1 Item: Title: Co-immunoprecipitation assays Destination: 1 Item: Title: Site-specific mutagenesis of CheW-like protein Destination: 1 Item: Title: Statistical analysis Destination: 1 Item: Title: Results Destination: 1 Children: Item: Title: RecA and CheW-like proteins are involved in the surface-associated motility and chemotaxis of A.baumannii strain ATCC 17978. Destination: 1 Item: Title: The lack of either RecA or cheW-like proteins reduces the virulence of A.baumannii strain ATCC 17978 in two different animal models. 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