3a80b8949ca759765c287356ddc18826 pmc_32268566.pdf d41e54735606a53eb98b94689d678f84979e4b21 pmc_32268566.pdf 98517db424a8800ab1fee0f02bf758e14f1a81a291fc5e7496bc7dc66f910e50 pmc_32268566.pdf Title: Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use Subject: Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions 2 log CFU/cm2) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2.07 log CFU/cm2). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4.90 log CFU/cm2) and S. aureus (3.84 log CFU/cm2). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5.80 log CFU/cm2) and S. aureus (4.11 log CFU/cm2). The results also showed a high correlation between concentration levels and the bacterial activity of Ag–ZnO-NPs (R2 = 0.97 for S. aureus, and R2 = 0.99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use. Keywords: nanoparticles; zinc oxide; silver; antimicrobial; biofilms; polyester; Escherichia coli; Staphylococcus aureus; Listeria monocytogenes Author: Fabio Fontecha-Umaña, Abel Guillermo Ríos-Castillo, Carolina Ripolles-Avila and José Juan Rodríguez-Jerez Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Mon Apr 6 14:18:25 2020 CEST ModDate: Mon Apr 6 14:18:25 2020 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 976729 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YVVFTQ+URWPalladioL-Ital Type 1 Custom yes yes no 93 0 PZCPAR+URWPalladioL-Bold Type 1 Custom yes yes no 94 0 YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 95 0 VEKLJW+URWPalladioL-Roma Type 1 Custom yes yes no 96 0 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