166ef8d19157e2abc8a65aac6f231f66 pmc_32326282.pdf e5cdc528f655eae6c755c1384b15a9505680674f pmc_32326282.pdf 51fae7849da165036423c1fe39bdb0ade35eee4ac7a5c28ab3a857e4ed8492e2 pmc_32326282.pdf Title: Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms Subject: Escherichia coli is considered one of the most common agents associated with neonatal diarrhea in piglets. The aim of this work was to characterize the pathogenic and antimicrobial resistance (AMR) profiles of 122 E. coli strains isolated from pigs suffering diarrhea (n = 94) and pigs without diarrhea (n = 28) of 24 farms in Spain. Virulence factors, toxins and AMR (ESBL and colistin) genes and AMR phenotypes of E. coli isolates were analyzed. Low prevalence of pathogenic E. coli strains (26%) was found in both groups. However, ETEC and VTEC strains were more frequently isolated from diarrheic piglets. Irrespectively of diarrhea occurrence, 97.5% of the strains showed a multidrug-resistance (MDR) profile to aminopenicillins, sulfonamides and tetracyclines. It was found that 22% of E. coli was CTX-M+, with CTX-M-14 being the principal allelic variant. Remarkably, 81.5% of CTX-M+ strains were isolated from diarrheic animals and presented an extended MDR profile to aminopenicillins, quinolones and aminoglycosides. Finally, low frequencies of colistin resistance genes mcr-1 (4/122) and mcr-4 (1/122) were found. MDR E. coli strains are circulating in pig farms of Spain, representing a serious threat to animal and public health. More appropriate diagnostic approaches (genetic and AMR phenotypic analysis) should be implemented in animal health to optimize antibiotic treatments. Keywords: antimicrobial resistance; ESBL genes; colistin resistance; Escherichia coli; neonatal diarrhea; pig Author: Anna Vidal, Laia Aguirre, Chiara Seminati, Montse Tello, Noelia Redondo, Marga Martín and Laila Darwich Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Tue Apr 21 14:16:21 2020 CEST ModDate: Tue Apr 21 14:16:21 2020 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 895234 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- VEWFKS+URWPalladioL-Ital Type 1 Custom yes yes no 89 0 AGXMPI+URWPalladioL-Bold Type 1 Custom yes yes no 90 0 SFUDNE+URWPalladioL-BoldItal Type 1 Custom yes yes no 91 0 WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes no 92 0 QTLELQ+Pxbsy Type 1 Builtin yes 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yes 242 0 PGMCKA+PalatinoLinotype TrueType WinAnsi yes yes no 285 0 PGMCNB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 286 0 PGMCND+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 287 0 PGMDHC+TimesNewRoman CID TrueType Identity-H yes yes yes 288 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 441 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:11:33 CEST RepresentationInformation: pmc_32326282.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:46:18 CEST Size: 895234 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 5 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone 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