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HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-GB Outlines: Item: Title: In Vitro and In Vivo Validation of EP2-Receptor Agonism to Selectively Achieve Inhibition of Mast Cell Activity Children: Item: Title: INTRODUCTION Item: Title: MATERIALS AND METHODS Children: Item: Title: Synthesis of the EP2 agonist CP-533536 Item: Title: Laboratory animals Item: Title: Murine and human MC populations Item: Title: MC stimulation and release assay Item: Title: Mice sensitization to house dust mite (HDM) aeroallergens Item: Title: Treatment with the EP2 agonist CP-533536 Item: Title: Assessment of airway hyper-responsiveness Item: Title: Assessment of airway inflammation Item: Title: Lung mouse MC protease-1 (mMCP-1) determination Item: Title: PCA Item: Title: Data and statistical analysis Item: Title: RESULTS Children: Item: Title: Effect of the selective prostanoid EP2 agonist butaprost on hIgE-mediated MC stimulation in vivo and in vitro Item: Title: In vivo and in vitro effects of the non-lipidic EP2 agonist CP-533536 on MC activation and airway sensitization Item: Title: DISCUSSION Item: Title: REFERENCES Info: Creator: Adobe InDesign CC 13.1 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Mon May 11 09:58:35 CEST 2020 ModDate: Mon May 11 09:58:42 CEST 2020 ID: 0x23ba09efbd22a241b094d3c09b5013ea, 0x030437bb3687114eb2ec3346d6fecd47 Filters: FilterPipeline: FlateDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 408 ImageHeight: 53 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Fonts: Type0: Font: BaseFont: PXEZDK+ArialUnicodeMS Encoding: Identity-H ToUnicode: true Font: BaseFont: UYGJVA+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: PTTFDK+TimesNewRomanPSMT Encoding: 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</tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.bold.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.first.bold</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.first.bold.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.first.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title1.bold</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title1.bold.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title1.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title2.bold</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title2.bold.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title2.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title3.bold</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title3.bold.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>body.title3.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Book Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>copyright</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name>National</tps:name> <tps:style>Book</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>corresp-email</tps:name> <tps:basedOn>*</tps:basedOn> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>crossref</tps:name> <tps:basedOn>*</tps:basedOn> <tps:leading>0 pt</tps:leading> <tps:color> C=15 M=100 Y=100 K=0 </tps:color> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>Fig.2</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>ExtraBold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> <tps:color> Black </tps:color> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>fig.bold</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> 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</tps:color> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>tableref</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name>National</tps:name> <tps:style>Bold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> <tps:color tint="90 %"> Black </tps:color> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>tbl.caption.bold</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>tbl.caption.bold.italic</tps:name> <tps:basedOn>*</tps:basedOn> <tps:font> <tps:name/> <tps:style>Bold Italic</tps:style> </tps:font> <tps:leading>0 pt</tps:leading> </tps:charStyleDef> </tps:definition> <tps:definition tag="charStyleDef"> <tps:charStyleDef> <tps:name>tbl.caption.italic</tps:name> 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