09e5b04ad8a4f6729ecd9d8221006036 pmc_32409652.pdf 05ca8861993d65d781bef5361af6a14c0dfeb53f pmc_32409652.pdf 31d625cba6c7421068754c62d615bea09e9c9ffaae967da043968c47772d48d3 pmc_32409652.pdf Title: Identification of circular RNAs in porcine sperm and evaluation of their relation to sperm motility Subject: Scientific Reports, doi:10.1038/s41598-020-64711-z Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Wed May 6 13:29:40 2020 CEST ModDate: Fri May 8 14:11:06 2020 CEST Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 2349533 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ADTKZY+Corbel-Bold CID TrueType Identity-H yes yes yes 67 0 YEHEXC+ArialUnicodeMS CID TrueType Identity-H yes yes yes 454 0 MinionPro-Regular Type 1C Custom yes no yes 69 0 CEBCOL+Corbel-Bold2 Type 1C WinAnsi yes yes yes 70 0 MinionPro-It Type 1C Custom yes no yes 71 0 KOCBQR+Corbel2 Type 1C WinAnsi yes yes yes 72 0 ELXSDQ+Corbel CID TrueType Identity-H yes yes yes 73 0 MyriadPro-It Type 1C WinAnsi yes no yes 74 0 KOCBQR+Corbel2 Type 1C WinAnsi yes yes yes 377 0 EuclidSymbol Type 1C Custom yes no yes 68 0 MinionPro-Bold Type 1C WinAnsi yes no yes 86 0 GLSKFM+STIXGeneral-Regular Type 1C Custom yes yes yes 306 0 GLSKFM+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 343 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-11-18 06:39:57 CET RepresentationInformation: pmc_32409652.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-06 03:44:28 CEST Size: 2349533 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 1. Genomic features of the circRNAs identified in the boar sperm. (a) Distribution of the genomic location (CDS, intergenic, intronic, 3′ UTR or 5′UTR) of the 1,598 circRNAs identified in the boar sperm. (b) Distribution of the number:57' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 1. Genomic features of the circRNAs identified in the boar sperm. (a) Distribution of the genomic location (CDS, intergenic, intronic, 3′ UTR or 5′UTR) of the 1,598 circRNAs identified in the boar sperm. (b) Distribution of the numbe:58' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 1. Genomic features of the circRNAs identified in the boar sperm. (a) Distribution of the genomic location (CDS, intergenic, intronic, 3′ UTR or 5′UTR) of the 1,598 circRNAs identified in the boar sperm. (b) Distribution of the numb:59' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 1. List of the 15 most abundant exonic circRNAs in swine sperm. circRNA genomic coordinates are indicated as chromosome:start_position..end_position. Mean and SD: standard deviation, are in CPM (Counts Per Million).:60' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 2. circRNA hotspot genes in swine sperm. 12 genes harbored 5 or more exonic circRNAs.:62' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 3. Concordance between the circRNAs catalogue of the boar sperm and the circRNA list in other species and pig tissues. Number of circRNAs after Sscrofa11.1 liftover from human, mice and swine (detailed by tissue). Column ‘Pig spe:67' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 3. Concordance between the circRNAs catalogue of the boar sperm and the circRNA list in other species and pig tissues. Number of circRNAs after Sscrofa11.1 liftover from human, mice and swine (detailed by tissue). Column ‘Pig sp:70' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 3. Concordance between the circRNAs catalogue of the boar sperm and the circRNA list in other species and pig tissues. Number of circRNAs after Sscrofa11.1 liftover from human, mice and swine (detailed by tissue). Column ‘Pig s:71' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 3. Concordance between the circRNAs catalogue of the boar sperm and the circRNA list in other species and pig tissues. Number of circRNAs after Sscrofa11.1 liftover from human, mice and swine (detailed by tissue). Column ‘Pig :72' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 2. CircRNA-miRNA interaction network. circRNA:miRNA relationships predicted by both RNA co-abundance and identification of miRNA target sites in the circRNAs sequences. Circular and square nodes represent circRNAs and miRNAs, respectively:77' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 2. CircRNA-miRNA interaction network. circRNA:miRNA relationships predicted by both RNA co-abundance and identification of miRNA target sites in the circRNAs sequences. Circular and square nodes represent circRNAs and miRNAs, respective:79' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 2. CircRNA-miRNA interaction network. circRNA:miRNA relationships predicted by both RNA co-abundance and identification of miRNA target sites in the circRNAs sequences. Circular and square nodes represent circRNAs and miRNAs, respectiv:80' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 23. Liang, G. M., Yang, Y. L., Niu, G. L., Tang, Z. L. & Li, K. Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages. DNA Res. 24, 523–535 (2017). :83' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 22. Glazar, P., Papavasileiou, P. & Rajewsky, N. circBase: a database for circular RNAs. RNA 20, 1666–1670 (2014). :84' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 3. Validation of the circRNAs which RNA-seq based abundance correlated with sperm motility. (a) Sanger sequencing validation of the circRNA black splice junction for ssc_circ_1132 from LIN7A, ssc_circ_1458 from LRBA and ssc_circ_132:86' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 3. Validation of the circRNAs which RNA-seq based abundance correlated with sperm motility. (a) Sanger sequencing validation of the circRNA black splice junction for ssc_circ_1132 from LIN7A, ssc_circ_1458 from LRBA and ssc_circ_1:88' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 3. Validation of the circRNAs which RNA-seq based abundance correlated with sperm motility. (a) Sanger sequencing validation of the circRNA black splice junction for ssc_circ_1132 from LIN7A, ssc_circ_1458 from LRBA and ssc_circ_:89' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 18. Chioccarelli, T. et al. Expression Patterns of Circular RNAs in High Quality and Poor Quality Human Spermatozoa. Front. Endocrinol. 10, 435 (2019). :90' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 1. Genomic features of the circRNAs identified in the boar sperm. (a) Distribution of the genomic location (CDS, intergenic, intronic, 3′ UTR or 5′UTR) of the 1,598 circRNAs identified in the boar sperm. (b) Distribution of the num:92' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 3. Concordance between the circRNAs catalogue of the boar sperm and the circRNA list in other species and pig tissues. Number of circRNAs after Sscrofa11.1 liftover from human, mice and swine (detailed by tissue). Column ‘Pig:96' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 3. Concordance between the circRNAs catalogue of the boar sperm and the circRNA list in other species and pig tissues. Number of circRNAs after Sscrofa11.1 liftover from human, mice and swine (detailed by tissue). Column ‘Pi:97' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 1. List of the 15 most abundant exonic circRNAs in swine sperm. circRNA genomic coordinates are indicated as chromosome:start_position..end_position. Mean and SD: standard deviation, are in CPM (Counts Per Million).:101' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 2. circRNA hotspot genes in swine sperm. 12 genes harbored 5 or more exonic circRNAs.:106' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 9. Gòdia, M., Swanson, G. & Krawetz, S. A. A history of why fathers’ RNA matters. Biol. Reprod. 99, 147–159 (2018). :110' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 24. Zhou, T. et al. Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages. RNA 24, 1443–1456 (2018). :112' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 2. CircRNA-miRNA interaction network. circRNA:miRNA relationships predicted by both RNA co-abundance and identification of miRNA target sites in the circRNAs sequences. Circular and square nodes represent circRNAs and miRNAs, respecti:115' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 2. circRNA hotspot genes in swine sperm. 12 genes harbored 5 or more exonic circRNAs.:128' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 3. Validation of the circRNAs which RNA-seq based abundance correlated with sperm motility. (a) Sanger sequencing validation of the circRNA black splice junction for ssc_circ_1132 from LIN7A, ssc_circ_1458 from LRBA and ssc_circ:134' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 3. Validation of the circRNAs which RNA-seq based abundance correlated with sperm motility. (a) Sanger sequencing validation of the circRNA black splice junction for ssc_circ_1132 from LIN7A, ssc_circ_1458 from LRBA and ssc_cir:138' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: Table 2. circRNA hotspot genes in swine sperm. 12 genes harbored 5 or more exonic circRNAs.:139' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 2. CircRNA-miRNA interaction network. circRNA:miRNA relationships predicted by both RNA co-abundance and identification of miRNA target sites in the circRNAs sequences. Circular and square nodes represent circRNAs and miRNAs, respect:141' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd:Figure 2. CircRNA-miRNA interaction network. circRNA:miRNA relationships predicted by both RNA co-abundance and identification of miRNA target sites in the circRNAs sequences. Circular and square nodes represent circRNAs and miRNAs, respec:145' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 21. Venø, M. T. et al. Spatio-temporal regulation of circular RNA expression during porcine embryonic brain development. Genome Biol. 16, 245 (2015). :147' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 23. Liang, G. M., Yang, Y. L., Niu, G. L., Tang, Z. L. & Li, K. Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages. DNA Res. 24, 523–535 (2017). :156' cannot be found Offset: 1774246 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_64711.indd: 22. Glazar, P., Papavasileiou, P. & Rajewsky, N. circBase: a database for circular RNAs. RNA 20, 1666–1670 (2014). :157' cannot be found Offset: 1774246 MIMEtype: application/pdf PDFMetadata: Objects: 550 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Identification of circular RNAs in porcine sperm and evaluation of their relation to sperm motility Children: Item: Title: Results Children: Item: Title: Phenotypic data. Item: Title: RNA isolation, RNA-seq and bioinformatics analysis. Item: Title: Characterization of the sperm circular RNA repertoire. Item: Title: Sperm circRNAs might be involved in epigenetic regulation and spermatogenesis. Item: Title: The boar sperm has a highly specific circRNAome. Item: Title: Sperm circRNAs do not follow an age-dependent pattern. Item: Title: circRNA-miRNA interaction network. Item: Title: Correlation of circRNAs with sperm motility and circRNA validation. Item: Title: Discussion Item: Title: Methods Children: Item: Title: Sperm collection and phenotyping. Item: Title: RNA isolation and library preparation. Item: Title: RNA-seq and bioinformatics analysis. Item: Title: circRNA-miRNA network visualization. Item: Title: Gene Ontology analysis and correlation with sperm motility parameters. Item: Title: Validation of circRNAs and reverse transcription quantitative PCR (RT-qPCR). Item: Title: Acknowledgements Item: Title: Figure 1 Genomic features of the circRNAs identified in the boar sperm. Item: Title: Figure 2 CircRNA-miRNA interaction network. Item: Title: Figure 3 Validation of the circRNAs which RNA-seq based abundance correlated with sperm motility. Item: Title: Table 1 List of the 15 most abundant exonic circRNAs in swine sperm. Item: Title: Table 2 circRNA hotspot genes in swine sperm. Item: Title: Table 3 Concordance between the circRNAs catalogue of the boar sperm and the circRNA list in other species and pig tissues. Info: Title: Identification of circular RNAs in porcine sperm and evaluation of their relation to sperm motility Subject: Scientific Reports, doi:10.1038/s41598-020-64711-z Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Wed May 06 13:59:40 CEST 2020 ModDate: Fri May 08 14:11:06 CEST 2020 ID: 0xd6ffcf387312fb4d5acd53db60fda2d3, 0x39c28cb1195bc584c467ec08d267772e Filters: FilterPipeline: FlateDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1900 ImageHeight: 646 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2000 ImageHeight: 1043 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1650 ImageHeight: 1494 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1100 ImageHeight: 90 ColorSpace: black is zero BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Fonts: Type0: Font: BaseFont: ADTKZY+Corbel-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: YEHEXC+ArialUnicodeMS Encoding: Identity-H ToUnicode: true Font: BaseFont: ELXSDQ+Corbel Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: GLSKFM+STIXGeneral-Regular FontSubset: true FirstChar: 31 LastChar: 31 FontDescriptor: FontName: GLSKFM+STIXGeneral-Regular Flags: Serif, Symbolic FontBBox: -995, -455, 2000, 1055 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: EuclidSymbol FirstChar: 0 LastChar: 255 FontDescriptor: FontName: EuclidSymbol Flags: Symbolic FontBBox: -166, -253, 1090, 950 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MinionPro-Regular FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Regular Flags: Serif, Nonsymbolic FontBBox: -290, -360, 1684, 989 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: CEBCOL+Corbel-Bold2 FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: CEBCOL+Corbel-Bold2 Flags: Nonsymbolic FontBBox: -556, -239, 1155, 952 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-Bold FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Bold Flags: Serif, Nonsymbolic FontBBox: -319, -360, 1684, 1024 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-It Flags: Serif, Nonsymbolic, Italic FontBBox: -201, -360, 1684, 1002 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: GLSKFM+TimesNewRomanPSMT FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: GLSKFM+TimesNewRomanPSMT Flags: Serif, Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: KOCBQR+Corbel2 FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: KOCBQR+Corbel2 Flags: Nonsymbolic FontBBox: -553, -239, 1110, 952 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MyriadPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MyriadPro-It Flags: Nonsymbolic, Italic FontBBox: -185, -250, 1090, 953 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true CIDFontType2: Font: BaseFont: ADTKZY+Corbel-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: ADTKZY+Corbel-Bold Flags: Symbolic FontBBox: -556, -239, 1155, 952 FontFile2: true Font: BaseFont: ELXSDQ+Corbel CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: ELXSDQ+Corbel Flags: Symbolic FontBBox: -553, -239, 1110, 952 FontFile2: true Font: BaseFont: YEHEXC+ArialUnicodeMS CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: YEHEXC+ArialUnicodeMS Flags: Symbolic FontBBox: -1011, -330, 2260, 1078 FontFile2: true XMP: application/pdf doi:10.1038/s41598-020-64711-z Springer US Scientific Reports, doi:10.1038/s41598-020-64711-z Identification of circular RNAs in porcine sperm and evaluation of their relation to sperm motility 10.1038/s41598-020-64711-z http://dx.doi.org/10.1038/s41598-020-64711-z journal Scientific Reports © 2020, The Author(s) 2045-2322 10.1038/s41598-020-64711-z Springer 2020-05-08T14:11:06+02:00 2020-05-06T16:59:40+05:30 2020-05-08T14:11:06+02:00 True iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; 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PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. In lieu of using #other please reach out to the PRISM group at prism-wg@yahoogroups.com to request addition of your term to the Platform Controlled Vocabulary. url Text http://www.niso.org/schemas/jav/1.0/ jav NISO external Values for Journal Article Version are one of the following: AO = Author’s Original SMUR = Submitted Manuscript Under Review AM = Accepted Manuscript P = Proof VoR = Version of Record CVoR = Corrected Version of Record EVoR = Enhanced Version of Record journal_article_version Closed Choice of Text Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 223, 25, 370, 16 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 208, 538, 217, 526 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 520, 527, 529, 513 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 302, 321, 304, 309 Flags: Print AppearanceDictionary: true Destination: 307 Annotation: Subtype: Link Rect: 271, 311, 274, 299 Flags: 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Annotation: Subtype: Link Rect: 223, 25, 370, 16 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 361, 399, 519, 387 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 349, 370, 441, 358 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 344, 261, 504, 249 Flags: Print AppearanceDictionary: true Thumb: true Checksum: 8e89a872 Type: CRC32 Checksum: 09e5b04ad8a4f6729ecd9d8221006036 Type: MD5 Checksum: 05ca8861993d65d781bef5361af6a14c0dfeb53f Type: SHA-1