c105d8669ea3f49497c0297453fb0e3b pmc_32423030.pdf e99bb365553d62572f44933f80adc9a2a5341480 pmc_32423030.pdf 282bc9c8ae9032e1523f2288a9da728f7cbd9f8e0df1059c67caf0b581a27494 pmc_32423030.pdf Title: Each Mycobacterium Requires a Specific Culture Medium Composition for Triggering an Optimized Immunomodulatory and Antitumoral Effect Subject: Mycobacterium bovis bacillus Calmette-Guérin (BCG) remains the first treatment option for non-muscle-invasive bladder cancer (BC) patients. In research laboratories, M. bovis BCG is mainly grown in commercially available media supplemented with animal-derived agents that favor its growth, while biomass production for patient treatment is performed in Sauton medium which lacks animal-derived components. However, there is not a standardized formulation of Sauton medium, which could affect mycobacterial characteristics. Here, the impact of culture composition on the immunomodulatory and antitumor capacity of M. bovis BCG and Mycolicibacterium brumae, recently described as efficacious for BC treatment, has been addressed. Both mycobacteria grown in Middlebrook and different Sauton formulations, differing in the source of nitrogen and amount of carbon source, were studied. Our results indicate the relevance of culture medium composition on the antitumor effect triggered by mycobacteria, indicating that the most productive culture medium is not necessarily the formulation that provides the most favorable immunomodulatory profile and the highest capacity to inhibit BC cell growth. Strikingly, each mycobacterial species requires a specific culture medium composition to provide the best profile as an immunotherapeutic agent for BC treatment. Our results highlight the relevance of meticulousness in mycobacteria production, providing insight into the application of these bacteria in BC research. Keywords: BCG; M. brumae; nonmuscle invasive; bladder cancer; Sauton; Middlebrook Author: Sandra Guallar-Garrido, Víctor Campo-Pérez, Alejandro Sánchez-Chardi, Marina Luquin and Esther Julián Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu May 14 13:59:19 2020 CEST ModDate: Thu May 14 13:59:19 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3332374 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UICBPZ+URWPalladioL-Ital Type 1 Custom yes yes no 104 0 XTBTTS+URWPalladioL-Bold Type 1 Custom yes yes no 105 0 LZDGTK+Rpxb Type 1 Builtin yes yes no 106 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 107 0 VESSJI+URWPalladioL-Roma Type 1 Custom yes yes no 108 0 TYUELJ+VnURWPalladioL Type 1 Custom yes yes no 109 0 HAKARX+Rpxr Type 1 Builtin yes yes no 110 0 SIXRFS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 115 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 132 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 133 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 134 0 KTOUYD+Rpxi Type 1 Builtin yes yes no 166 0 DFIRNB+Pxsy Type 1 Builtin yes yes no 190 0 YICMPF+Rpcxr Type 1 Builtin yes yes no 191 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 207 0 FPCWAK+Palatino Linotype Bold CID TrueType Identity-H yes yes yes 223 0 FPRZGR+Palatino Linotype CID TrueType Identity-H yes yes yes 224 0 FPVQLD+Times New Roman CID TrueType Identity-H yes yes yes 225 0 FPEYRU+Palatino Linotype Italic CID TrueType Identity-H yes yes yes 226 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 262 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 263 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 264 0 Times New Roman TrueType WinAnsi no no no 265 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 290 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 291 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 292 0 Times New Roman TrueType WinAnsi no no no 293 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 294 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 326 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 327 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 328 0 Times New Roman TrueType WinAnsi no no no 329 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 330 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 364 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 365 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 366 0 Times New Roman TrueType WinAnsi no no no 367 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 368 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 405 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 406 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 407 0 Times New Roman TrueType WinAnsi no no no 408 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 409 0 AWDAAW+VnURWPalladioL-Italic Type 1 Custom yes yes no 591 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:43:24 CEST RepresentationInformation: pmc_32423030.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 11:46:31 CEST Size: 3332374 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰きづつどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぬぜ〴ぜ〰こぴひちどのびぜ〴ぜ〰ちのつぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰てはひぜ〴ぜ〰さぬぴひちびぴひふっぴふひちぬぜ〴ぜ〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きべっはぢちっぴづひどちぬぜ〴ぜ〰ぐづぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰あちっどぬぬどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごふねはひぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぉのとどぢどぴどはのぜ〴ぜ〰ぅへばづひどねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きべっはぢちっぴづひどちぬぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのびどつづぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰きづつどちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぬぜ〴ぜ〰こぴひちどのびぜ〴ぜ〰ちのつぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰てはひぜ〴ぜ〰さぬぴひちびぴひふっぴふひちぬぜ〴ぜ〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きべっはぢちっぴづひどちぬぜ〴ぜ〰ぐづぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰あちっどぬぬどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごふねはひぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぉのとどぢどぴどはのぜ〴ぜ〰ぅへばづひどねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きべっはぢちっぴづひどちぬぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのびどつづぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: あちっぴづひどちぬぜ〴ぜ〰こぴひちどのびぜ〴ぜ〰ちのつぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰てはひぜ〴ぜ〰さぬぴひちびぴひふっぴふひちぬぜ〴ぜ〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きべっはぢちっぴづひどちぬぜ〴ぜ〰ぐづぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰あちっどぬぬどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごふねはひぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぉのとどぢどぴどはのぜ〴ぜ〰ぅへばづひどねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きべっはぢちっぴづひどちぬぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのびどつづぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぐひづばちひちぴどはのぜ〴ぜ〰てはひぜ〴ぜ〰さぬぴひちびぴひふっぴふひちぬぜ〴ぜ〰ぁびびづびびねづのぴぜ〴ぜ〰はてぜ〴ぜ〰きべっはぢちっぴづひどちぬぜ〴ぜ〰ぐづぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰あちっどぬぬどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごふねはひぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぉのとどぢどぴどはのぜ〴ぜ〰ぅへばづひどねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きべっはぢちっぴづひどちぬぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのびどつづぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごふねはひぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぉのとどぢどぴどはのぜ〴ぜ〰ぅへばづひどねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きべっはぢちっぴづひどちぬぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのびどつづぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ごふねはひぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰ぉのとどぢどぴどはのぜ〴ぜ〰ぅへばづひどねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きべっはぢちっぴづひどちぬぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのびどつづぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: きべっはぢちっぴづひどちぬぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのびどつづぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃべぴはにどのづぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐひづびづのっづぜ〴ぜ〰はてぜ〴ぜ〰ずのこく〴ぜ〴ぜ〰ちのつぜ〴ぜ〰が〭ぁびばちひちでどのづぜ〴ぜ〰ちのつぜ〴ぜ〰ぉのっひづちびどのでぜ〴ぜ〰ぇぬべっづひはぬぜ〴ぜ〰ぃはのっづのぴひちぴどはのびぜ〴ぜ〰どのぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰きづつどちぜ〴ぜ〰いづぴづひねどのづぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰はてぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぃふぬぴふひづぜ〴ぜ〰ぃはねばはびどぴどはのぜ〴ぜ〰いどててづひづのぴどちぬぬべぜ〴ぜ〰ぉのてぬふづのっづびぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴ぜ〰あどはねちびびぜ〴ぜ〰ぐひはつふっぴどはのぜ〴ぜ〰ぢふぴぜ〴ぜ〰ぎはぴぜ〴ぜ〰どのぜ〴ぜ〰ごづひねびぜ〴ぜ〰はてぜ〴ぜ〰きどっひはびっはばどっぜ〴ぜ〰ぁばばづちひちのっづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴ぜ〰ぁててづっぴぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉねねふのづぜ〴ぜ〰げづびばはのびづぜ〴ぜ〰ごひどででづひづつぜ〴ぜ〰ぢべぜ〴ぜ〰きべっはぢちっぴづひどちぜ〴ぜ〰ぢふぴぜ〴ぜ〰いはぜ〴ぜ〰ぎはぴぜ〴ぜ〰きはつどてべぜ〴ぜ〰ごとづどひぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁのぴどぴふねはひぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰どびぜ〴ぜ〰ぅのとちのっづつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぁびばちひちでどのづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどふねぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぇぬふぴちねちぴづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどちぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ぐひづびづのっづぜ〴ぜ〰はてぜ〴ぜ〰ずのこく〴ぜ〴ぜ〰ちのつぜ〴ぜ〰が〭ぁびばちひちでどのづぜ〴ぜ〰ちのつぜ〴ぜ〰ぉのっひづちびどのでぜ〴ぜ〰ぇぬべっづひはぬぜ〴ぜ〰ぃはのっづのぴひちぴどはのびぜ〴ぜ〰どのぜ〴ぜ〰ぃふぬぴふひづぜ〴ぜ〰きづつどちぜ〴ぜ〰いづぴづひねどのづぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰はてぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぃふぬぴふひづぜ〴ぜ〰ぃはねばはびどぴどはのぜ〴ぜ〰いどててづひづのぴどちぬぬべぜ〴ぜ〰ぉのてぬふづのっづびぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴ぜ〰あどはねちびびぜ〴ぜ〰ぐひはつふっぴどはのぜ〴ぜ〰ぢふぴぜ〴ぜ〰ぎはぴぜ〴ぜ〰どのぜ〴ぜ〰ごづひねびぜ〴ぜ〰はてぜ〴ぜ〰きどっひはびっはばどっぜ〴ぜ〰ぁばばづちひちのっづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴ぜ〰ぁててづっぴぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉねねふのづぜ〴ぜ〰げづびばはのびづぜ〴ぜ〰ごひどででづひづつぜ〴ぜ〰ぢべぜ〴ぜ〰きべっはぢちっぴづひどちぜ〴ぜ〰ぢふぴぜ〴ぜ〰いはぜ〴ぜ〰ぎはぴぜ〴ぜ〰きはつどてべぜ〴ぜ〰ごとづどひぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁのぴどぴふねはひぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰どびぜ〴ぜ〰ぅのとちのっづつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぁびばちひちでどのづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどふねぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぇぬふぴちねちぴづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどちぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃふぬぴふひづぜ〴ぜ〰ぃはねばはびどぴどはのぜ〴ぜ〰いどててづひづのぴどちぬぬべぜ〴ぜ〰ぉのてぬふづのっづびぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴ぜ〰あどはねちびびぜ〴ぜ〰ぐひはつふっぴどはのぜ〴ぜ〰ぢふぴぜ〴ぜ〰ぎはぴぜ〴ぜ〰どのぜ〴ぜ〰ごづひねびぜ〴ぜ〰はてぜ〴ぜ〰きどっひはびっはばどっぜ〴ぜ〰ぁばばづちひちのっづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴ぜ〰ぁててづっぴぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉねねふのづぜ〴ぜ〰げづびばはのびづぜ〴ぜ〰ごひどででづひづつぜ〴ぜ〰ぢべぜ〴ぜ〰きべっはぢちっぴづひどちぜ〴ぜ〰ぢふぴぜ〴ぜ〰いはぜ〴ぜ〰ぎはぴぜ〴ぜ〰きはつどてべぜ〴ぜ〰ごとづどひぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁのぴどぴふねはひぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰どびぜ〴ぜ〰ぅのとちのっづつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぁびばちひちでどのづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどふねぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぇぬふぴちねちぴづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどちぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃふぬぴふひづぜ〴ぜ〰ぃはのつどぴどはのびぜ〴ぜ〰ぁててづっぴぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉねねふのづぜ〴ぜ〰げづびばはのびづぜ〴ぜ〰ごひどででづひづつぜ〴ぜ〰ぢべぜ〴ぜ〰きべっはぢちっぴづひどちぜ〴ぜ〰ぢふぴぜ〴ぜ〰いはぜ〴ぜ〰ぎはぴぜ〴ぜ〰きはつどてべぜ〴ぜ〰ごとづどひぜ〴ぜ〰こふひぶどぶちぬぜ〴ぜ〰どのぜ〴ぜ〰きちっひはばとちでづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぁのぴどぴふねはひぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰どびぜ〴ぜ〰ぅのとちのっづつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぁびばちひちでどのづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどふねぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぇぬふぴちねちぴづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどちぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぁのぴどぴふねはひぜ〴ぜ〰ぁっぴどぶどぴべぜ〴ぜ〰どびぜ〴ぜ〰ぅのとちのっづつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぁびばちひちでどのづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどふねぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢひふねちづぜ〴ぜ〰ちのつぜ〴ぜ〰どのぜ〴ぜ〰が〭ぇぬふぴちねちぴづ〭ぃはのぴちどのどのでぜ〴ぜ〰きづつどちぜ〴ぜ〰てはひぜ〴ぜ〰き〮ぜ〴ぜ〰ぢはぶどびぜ〴ぜ〰あぃぇぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⹁⸩‾㸊⠩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⹁⸩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: KTOUYD+Rpxi FontSubset: true FirstChar: 11 LastChar: 60 FontDescriptor: FontName: KTOUYD+Rpxi Flags: Symbolic FontBBox: -162, -280, 856, 733 FontFile: true Font: BaseFont: UICBPZ+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: UICBPZ+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XTBTTS+URWPalladioL-Bold FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: XTBTTS+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: LZDGTK+Rpxb FontSubset: true FirstChar: 11 LastChar: 11 FontDescriptor: FontName: LZDGTK+Rpxb Flags: Symbolic FontBBox: -57, -266, 932, 729 FontFile: true Font: BaseFont: IOJCNK+VnURWPalladioL-Bold FontSubset: true FirstChar: 161 LastChar: 189 FontDescriptor: FontName: IOJCNK+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: VESSJI+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 248 FontDescriptor: FontName: VESSJI+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TYUELJ+VnURWPalladioL FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: TYUELJ+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HAKARX+Rpxr FontSubset: true FirstChar: 11 LastChar: 60 FontDescriptor: FontName: HAKARX+Rpxr Flags: Symbolic FontBBox: -40, -283, 904, 728 FontFile: true Font: BaseFont: DFIRNB+Pxsy FontSubset: true FirstChar: 0 LastChar: 48 FontDescriptor: FontName: DFIRNB+Pxsy Flags: Symbolic FontBBox: -21, -994, 1292, 912 FontFile: true Font: BaseFont: YICMPF+Rpcxr FontSubset: true FirstChar: 151 LastChar: 174 FontDescriptor: FontName: YICMPF+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: VVSYYB+EURM10 FontSubset: true FirstChar: 11 LastChar: 22 FontDescriptor: FontName: VVSYYB+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: AWDAAW+VnURWPalladioL-Italic FontSubset: true FirstChar: 160 LastChar: 160 FontDescriptor: FontName: AWDAAW+VnURWPalladioL-Italic Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 75, 757, 238, 793 Annotation: Subtype: Link Rect: 474, 757, 519, 793 Annotation: Subtype: Link Rect: 190, 640, 201, 654 Annotation: Subtype: Link Rect: 309, 640, 321, 654 Annotation: Subtype: Link Rect: 458, 640, 469, 654 Annotation: Subtype: Link Rect: 151, 626, 162, 640 Annotation: Subtype: Link Rect: 255, 626, 266, 640 Annotation: Subtype: Link Rect: 466, 475, 518, 494 Annotation: Subtype: Link Rect: 384, 86, 391, 95 ActionDest: 525 Annotation: Subtype: Link Rect: 216, 72, 223, 81 ActionDest: 525 Annotation: Subtype: Link Rect: 187, 38, 302, 50 Annotation: Subtype: Link Rect: 374, 38, 519, 50 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 292, 742, 299, 752 ActionDest: 525 Annotation: Subtype: Link Rect: 114, 715, 121, 725 ActionDest: 525 Annotation: Subtype: Link Rect: 278, 594, 285, 603 ActionDest: 525 Annotation: Subtype: Link Rect: 286, 594, 293, 603 ActionDest: 525 Annotation: Subtype: Link Rect: 124, 537, 134, 549 ActionDest: 493 Annotation: Subtype: Link Rect: 141, 540, 148, 549 ActionDest: 525 Annotation: Subtype: Link Rect: 150, 540, 157, 549 ActionDest: 525 Annotation: Subtype: Link Rect: 254, 418, 266, 427 ActionDest: 525 Annotation: Subtype: Link Rect: 267, 418, 279, 427 ActionDest: 525 Annotation: Subtype: Link Rect: 373, 405, 385, 414 ActionDest: 525 Annotation: Subtype: Link Rect: 389, 405, 400, 414 ActionDest: 525 Annotation: Subtype: Link Rect: 466, 351, 478, 360 ActionDest: 562 Annotation: Subtype: Link Rect: 281, 324, 293, 333 ActionDest: 525 Annotation: Subtype: Link Rect: 425, 256, 437, 265 ActionDest: 525 Annotation: Subtype: Link Rect: 437, 256, 449, 265 ActionDest: 562 Annotation: Subtype: Link Rect: 452, 256, 464, 265 ActionDest: 562 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 176, 702, 183, 711 ActionDest: 525 Annotation: Subtype: Link Rect: 159, 605, 171, 614 ActionDest: 562 Annotation: Subtype: Link Rect: 372, 373, 383, 383 ActionDest: 562 Annotation: Subtype: Link Rect: 386, 373, 398, 383 ActionDest: 562 Annotation: Subtype: Link Rect: 442, 180, 449, 189 ActionDest: 525 Annotation: Subtype: Link Rect: 449, 180, 461, 189 ActionDest: 562 Annotation: Subtype: Link Rect: 462, 180, 474, 189 ActionDest: 562 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 109, 129, 115, 141 ActionDest: 217 Annotation: Subtype: Link Rect: 284, 102, 290, 114 ActionDest: 217 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 219, 726, 226, 738 ActionDest: 217 Annotation: Subtype: Link Rect: 436, 141, 443, 153 ActionDest: 217 Annotation: Subtype: Link Rect: 504, 114, 510, 126 ActionDest: 256 Annotation: Subtype: Link Rect: 238, 87, 245, 99 ActionDest: 256 Annotation: Subtype: Link Rect: 110, 73, 117, 85 ActionDest: 256 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 470, 740, 477, 752 ActionDest: 256 Annotation: Subtype: Link Rect: 185, 699, 192, 711 ActionDest: 256 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 388, 726, 395, 738 ActionDest: 319 Annotation: Subtype: Link Rect: 305, 686, 312, 698 ActionDest: 319 Annotation: Subtype: Link Rect: 384, 632, 391, 644 ActionDest: 319 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 406, 459, 414, 471 ActionDest: 319 Annotation: Subtype: Link Rect: 388, 337, 395, 349 ActionDest: 357 Annotation: Subtype: Link Rect: 110, 189, 117, 200 ActionDest: 357 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 109, 180, 116, 192 ActionDest: 399 Annotation: Subtype: Link Rect: 182, 153, 189, 165 ActionDest: 399 Annotation: Subtype: Link Rect: 416, 126, 423, 138 ActionDest: 399 Annotation: Subtype: Link Rect: 384, 72, 391, 84 ActionDest: 399 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 211, 234, 218, 243 ActionDest: 525 Annotation: Subtype: Link Rect: 221, 234, 228, 243 ActionDest: 525 Annotation: Subtype: Link Rect: 263, 139, 270, 148 ActionDest: 525 Annotation: Subtype: Link Rect: 270, 139, 282, 148 ActionDest: 525 Annotation: Subtype: Link Rect: 283, 139, 295, 148 ActionDest: 562 Annotation: Subtype: Link Rect: 295, 139, 307, 148 ActionDest: 562 Annotation: Subtype: Link Rect: 308, 139, 320, 148 ActionDest: 562 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 363, 742, 375, 752 ActionDest: 562 Annotation: Subtype: Link Rect: 340, 702, 352, 711 ActionDest: 562 Annotation: Subtype: Link Rect: 352, 702, 364, 711 ActionDest: 562 Annotation: Subtype: Link Rect: 357, 621, 369, 630 ActionDest: 562 Annotation: Subtype: Link Rect: 372, 621, 384, 630 ActionDest: 562 Annotation: Subtype: Link Rect: 391, 594, 403, 603 ActionDest: 562 Annotation: Subtype: Link Rect: 387, 567, 399, 576 ActionDest: 562 Annotation: Subtype: Link Rect: 191, 513, 203, 522 ActionDest: 562 Annotation: Subtype: Link Rect: 204, 513, 216, 522 ActionDest: 562 Annotation: Subtype: Link Rect: 219, 513, 231, 522 ActionDest: 586 Annotation: Subtype: Link Rect: 273, 499, 285, 508 ActionDest: 586 Annotation: Subtype: Link Rect: 285, 499, 297, 508 ActionDest: 586 Annotation: Subtype: Link Rect: 208, 486, 220, 495 ActionDest: 586 Annotation: Subtype: Link Rect: 464, 472, 476, 481 ActionDest: 586 Annotation: Subtype: Link Rect: 338, 459, 350, 468 ActionDest: 562 Annotation: Subtype: Link Rect: 140, 297, 152, 306 ActionDest: 586 Annotation: Subtype: Link Rect: 440, 256, 452, 265 ActionDest: 562 Annotation: Subtype: Link Rect: 453, 256, 465, 265 ActionDest: 562 Annotation: Subtype: Link Rect: 399, 240, 406, 252 ActionDest: 319 Annotation: Subtype: Link Rect: 504, 243, 516, 252 ActionDest: 586 Annotation: Subtype: Link Rect: 503, 229, 515, 238 ActionDest: 562 Annotation: Subtype: Link Rect: 138, 202, 150, 211 ActionDest: 586 Annotation: Subtype: Link Rect: 346, 202, 358, 211 ActionDest: 586 Annotation: Subtype: Link Rect: 330, 175, 342, 184 ActionDest: 586 Annotation: Subtype: Link Rect: 342, 175, 354, 184 ActionDest: 586 Annotation: Subtype: Link Rect: 123, 161, 135, 171 ActionDest: 586 Annotation: Subtype: Link Rect: 138, 161, 150, 171 ActionDest: 586 Annotation: Subtype: Link Rect: 140, 148, 152, 157 ActionDest: 586 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 179, 743, 191, 752 ActionDest: 525 Annotation: Subtype: Link Rect: 192, 742, 204, 752 ActionDest: 525 Annotation: Subtype: Link Rect: 204, 742, 216, 752 ActionDest: 586 Annotation: Subtype: Link Rect: 114, 715, 126, 725 ActionDest: 586 Annotation: Subtype: Link Rect: 285, 634, 297, 644 ActionDest: 562 Annotation: Subtype: Link Rect: 291, 594, 303, 603 ActionDest: 586 Annotation: Subtype: Link Rect: 306, 594, 318, 603 ActionDest: 599 Annotation: Subtype: Link Rect: 332, 297, 505, 308 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 436, 721, 519, 732 Annotation: Subtype: Link Rect: 97, 708, 331, 719 Annotation: Subtype: Link Rect: 150, 670, 187, 680 Annotation: Subtype: Link Rect: 100, 632, 137, 642 Annotation: Subtype: Link Rect: 143, 632, 179, 642 Annotation: Subtype: Link Rect: 268, 592, 306, 603 Annotation: Subtype: Link Rect: 243, 553, 281, 564 Annotation: Subtype: Link Rect: 287, 553, 323, 564 Annotation: Subtype: Link Rect: 406, 527, 443, 538 Annotation: Subtype: Link Rect: 450, 527, 486, 538 Annotation: Subtype: Link Rect: 347, 489, 384, 500 Annotation: Subtype: Link Rect: 223, 451, 260, 461 Annotation: Subtype: Link Rect: 229, 411, 266, 422 Annotation: Subtype: Link Rect: 150, 386, 187, 396 Annotation: Subtype: Link Rect: 446, 360, 483, 370 Annotation: Subtype: Link Rect: 233, 309, 271, 319 Annotation: Subtype: Link Rect: 277, 309, 313, 319 Annotation: Subtype: Link Rect: 325, 269, 362, 280 Annotation: Subtype: Link Rect: 364, 230, 401, 241 Annotation: Subtype: Link Rect: 408, 230, 444, 241 Annotation: Subtype: Link Rect: 240, 205, 277, 216 Annotation: Subtype: Link Rect: 284, 205, 320, 216 Annotation: Subtype: Link Rect: 463, 192, 500, 203 Annotation: Subtype: Link Rect: 479, 166, 516, 177 Annotation: Subtype: Link Rect: 286, 102, 324, 112 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 229, 714, 266, 725 Annotation: Subtype: Link Rect: 480, 675, 516, 686 Annotation: Subtype: Link Rect: 294, 650, 330, 661 Annotation: Subtype: Link Rect: 159, 625, 196, 635 Annotation: Subtype: Link Rect: 202, 625, 238, 635 Annotation: Subtype: Link Rect: 186, 586, 223, 596 Annotation: Subtype: Link Rect: 229, 586, 265, 596 Annotation: Subtype: Link Rect: 310, 533, 347, 544 Annotation: Subtype: Link Rect: 354, 533, 390, 544 Annotation: Subtype: Link Rect: 157, 444, 195, 454 Annotation: Subtype: Link Rect: 231, 405, 268, 415 Annotation: Subtype: Link Rect: 215, 366, 253, 376 Annotation: Subtype: Link Rect: 465, 340, 502, 351 Annotation: Subtype: Link Rect: 271, 314, 308, 325 Annotation: Subtype: Link Rect: 372, 249, 410, 260 Annotation: Subtype: Link Rect: 416, 249, 452, 260 Annotation: Subtype: Link Rect: 235, 211, 272, 222 Annotation: Subtype: Link Rect: 279, 211, 315, 222 Annotation: Subtype: Link Rect: 341, 185, 378, 196 Annotation: Subtype: Link Rect: 226, 160, 264, 170 Annotation: Subtype: Link Rect: 270, 160, 306, 170 Annotation: Subtype: Link Rect: 348, 134, 385, 144 Annotation: Subtype: Link Rect: 391, 134, 427, 144 Annotation: Subtype: Link Rect: 141, 95, 178, 105 Annotation: Subtype: Link Rect: 322, 69, 359, 80 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 366, 727, 403, 738 Annotation: Subtype: Link Rect: 385, 676, 423, 686 Annotation: Subtype: Link Rect: 300, 637, 338, 648 Annotation: Subtype: Link Rect: 344, 637, 380, 648 Annotation: Subtype: Link Rect: 100, 599, 137, 609 Annotation: Subtype: Link Rect: 143, 599, 179, 609 Annotation: Subtype: Link Rect: 300, 573, 337, 583 Annotation: Subtype: Link Rect: 343, 573, 379, 583 Annotation: Subtype: Link Rect: 179, 508, 217, 519 Annotation: Subtype: Link Rect: 474, 456, 512, 467 Annotation: Subtype: Link Rect: 418, 417, 455, 428 Annotation: Subtype: Link Rect: 100, 379, 137, 389 Annotation: Subtype: Link Rect: 163, 341, 200, 351 Annotation: Subtype: Link Rect: 397, 314, 434, 325 Annotation: Subtype: Link Rect: 239, 249, 275, 260 Annotation: Subtype: Link Rect: 277, 172, 315, 183 Annotation: Subtype: Link Rect: 321, 172, 357, 183 Annotation: Subtype: Link Rect: 296, 147, 334, 157 Page: Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 467, 727, 505, 738 Annotation: Subtype: Link Rect: 326, 688, 364, 699 Annotation: Subtype: Link Rect: 76, 646, 166, 677 Annotation: Subtype: Link Rect: 234, 641, 407, 652 Checksum: d4bc5316 Type: CRC32 Checksum: c105d8669ea3f49497c0297453fb0e3b Type: MD5 Checksum: e99bb365553d62572f44933f80adc9a2a5341480 Type: SHA-1