f1dfb5cdb21a2668e13fdf476d8cd6cd pmc_32523561.pdf b4cba86ad207eb693c0c9de1ed9c408226fbb75c pmc_32523561.pdf 4beea841bf99fcc032d183ccda28a67771506207c2bbaef77c4ebb24ca2bcc55 pmc_32523561.pdf Title: Whole Genome Sequencing of Hepatitis A Virus Using a PCR-free Single-Molecule Nanopore Sequencing Approach Subject: Hepatitis A virus (HAV) is one of the most common causes of acute viral hepatitis in humans. Keywords: hepatitis A virus, Picornaviridae, genome, nanopore sequencing, metagenomics, SNV Author: Frederico M. Batista Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed May 20 12:33:22 2020 CEST ModDate: Wed May 20 12:33:22 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 1356311 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DVOZDH+MinionPro-Regular Type 1 Custom yes yes no 30 0 DLXMHT+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 31 0 OXJAMS+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 32 0 CCVXXJ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 37 0 GYKLHP+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 38 0 DFNPCA+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 39 0 TTPJTX+MinionPro-It Type 1 Custom yes yes no 106 0 FZMHIH+MTSYN Type 1 Builtin yes yes no 107 0 JYDOCF+MTGU Type 1 Builtin yes yes no 120 0 RQWPEG+MinionPro-Bold Type 1 Custom yes yes no 188 0 XODHDR+RBLMI Type 1 Builtin yes yes no 230 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:42:51 CEST RepresentationInformation: pmc_32523561.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 11:46:44 CEST Size: 1356311 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 527 FreeObjects: 2 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Whole Genome Sequencing of Hepatitis A Virus Using a PCR-Free Single-Molecule Nanopore Sequencing Approach Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Sample Preparation Item: Title: HAV Quantification Item: Title: RNA Extraction for Sequencing Item: Title: Direct cDNA Library Preparation and Nanopore Sequencing Item: Title: Amplicon Library Preparation and Nanopore Sequencing Item: Title: Bioinformatic Analysis Item: Title: Results and Discussion Children: Item: Title: RNA Extraction and Purification Item: Title: Direct cDNA Nanopore Sequencing Item: Title: HAV Genetic Diversity Item: Title: Conclusion Item: Title: Data Availability Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: References Info: Title: Whole Genome Sequencing of Hepatitis A Virus Using a PCR-free Single-Molecule Nanopore Sequencing Approach Author: Frederico M. Batista Subject: Hepatitis A virus (HAV) is one of the most common causes of acute viral hepatitis in humans. Keywords: hepatitis A virus, Picornaviridae, genome, nanopore sequencing, metagenomics, SNV Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed May 20 13:03:22 CEST 2020 ModDate: Wed May 20 13:03:22 CEST 2020 ID: 0x930a7bef17e3807bd1ccdf2f0a99c7ee, 0x930a7bef17e3807bd1ccdf2f0a99c7ee Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: OXJAMS+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: OXJAMS+HelveticaNeueLTStd-Lt Flags: Symbolic FontBBox: -166, -214, 1050, 967 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: CCVXXJ+HelveticaNeueLTStd-BdIt FontSubset: true FirstChar: 42 LastChar: 121 FontDescriptor: FontName: CCVXXJ+HelveticaNeueLTStd-BdIt Flags: Symbolic FontBBox: -166, -218, 1129, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GYKLHP+HelveticaNeueLTStd-LtIt FontSubset: true FirstChar: 2 LastChar: 242 FontDescriptor: FontName: GYKLHP+HelveticaNeueLTStd-LtIt Flags: Symbolic FontBBox: -165, -214, 1099, 951 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XODHDR+RBLMI FontSubset: true FirstChar: 60 LastChar: 62 FontDescriptor: FontName: XODHDR+RBLMI Flags: Symbolic FontBBox: -11, -230, 987, 750 FontFile: true Font: BaseFont: DFNPCA+HelveticaNeueLTStd-MdIt FontSubset: true FirstChar: 42 LastChar: 122 FontDescriptor: FontName: DFNPCA+HelveticaNeueLTStd-MdIt Flags: Symbolic FontBBox: -165, -218, 1125, 947 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: JYDOCF+MTGU FontSubset: true FirstChar: 109 LastChar: 109 FontDescriptor: FontName: JYDOCF+MTGU Flags: Symbolic FontBBox: 25, -213, 839, 683 FontFile: true Font: BaseFont: TTPJTX+MinionPro-It FontSubset: true FirstChar: 2 LastChar: 169 FontDescriptor: FontName: TTPJTX+MinionPro-It Flags: Symbolic FontBBox: -201, -360, 1684, 919 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: FZMHIH+MTSYN FontSubset: true FirstChar: 0 LastChar: 67 FontDescriptor: FontName: FZMHIH+MTSYN Flags: Symbolic FontBBox: 0, -954, 1043, 900 FontFile: true Font: BaseFont: RQWPEG+MinionPro-Bold FontSubset: true FirstChar: 3 LastChar: 117 FontDescriptor: FontName: RQWPEG+MinionPro-Bold Flags: Symbolic FontBBox: -318, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: DVOZDH+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 233 FontDescriptor: FontName: DVOZDH+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: DLXMHT+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: DLXMHT+HelveticaNeueLTStd-Bd Flags: Symbolic FontBBox: -166, -218, 1078, 975 FontFile: true EncodingDictionary: Differences: true XMP: hepatitis A virus, Picornaviridae, genome, nanopore sequencing, metagenomics, SNV pdfTeX-1.40.15 1.5 application/pdf Whole Genome Sequencing of Hepatitis A Virus Using a PCR-free Single-Molecule Nanopore Sequencing Approach Hepatitis A virus (HAV) is one of the most common causes of acute viral hepatitis in humans. Frederico M. 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