bf4e9bbe1b9243b7d7d06476f4ac94a2 pmc_32645906.pdf d5f4e77b05843d03909b3082c20669a44a643071 pmc_32645906.pdf 079c8c5d79b386db93508fb599a15da96b37f8774417eb2d858eba9ce3a15aa2 pmc_32645906.pdf Title: Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits Subject: Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; / hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10–72 h post-mating. In Experiment 1, does (n = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does (n = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial CTEN and NGF by NM, but not by SP-AI. The findings suggest that the NGF gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development. Keywords: gene expression; qPCR; female reproductive tract; induced ovulator Author: Jaume Gardela, Amaia Jauregi-Miguel, Cristina A. Martinez, Heriberto Rodriguez-Martinez, Manel Lopez-Bejar and Manuel Alvarez-Rodriguez Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Tue Jul 7 12:04:21 2020 CEST ModDate: Tue Jul 7 12:04:21 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1277950 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QZBOSC+URWPalladioL-Ital Type 1 Custom yes yes no 108 0 RLMQIB+URWPalladioL-Bold Type 1 Custom yes yes no 109 0 ACBFSH+URWPalladioL-Roma Type 1 Custom yes yes no 110 0 CMFVHJ+VnURWPalladioL Type 1 Custom yes yes no 111 0 IELMKZ+Rpxr Type 1 Builtin yes yes no 112 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 113 0 HIZBRM+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 118 0 KONDUX+EuphemiaUCAS-Bold Type 1C WinAnsi yes yes no 119 0 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TrueType WinAnsi yes yes no 318 0 KBEECD+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 319 0 KBEENP+TimesNewRoman CID TrueType Identity-H yes yes yes 320 0 KBEDOA+PalatinoLinotype TrueType WinAnsi yes yes no 349 0 KBEEBB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 350 0 KBEECD+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 351 0 KBEENP+TimesNewRoman CID TrueType Identity-H yes yes yes 352 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:42:03 CEST RepresentationInformation: pmc_32645906.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 11:46:58 CEST Size: 1277950 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぅぴとどっびぜ〴ぜ〰こぴちぴづねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぁのどねちぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぅへばづひどねづのぴちぬぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きちぴどのでぜ〴ぜ〰ちのつぜ〴ぜ〰こづねづのぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどぴちぴどぶづぜ〴ぜ〰ぐぃげぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぅぴとどっびぜ〴ぜ〰こぴちぴづねづのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぁのどねちぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぅへばづひどねづのぴちぬぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きちぴどのでぜ〴ぜ〰ちのつぜ〴ぜ〰こづねづのぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどぴちぴどぶづぜ〴ぜ〰ぐぃげぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぁのどねちぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぅへばづひどねづのぴちぬぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きちぴどのでぜ〴ぜ〰ちのつぜ〴ぜ〰こづねづのぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどぴちぴどぶづぜ〴ぜ〰ぐぃげぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぅへばづひどねづのぴちぬぜ〴ぜ〰いづびどでのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰きちぴどのでぜ〴ぜ〰ちのつぜ〴ぜ〰こづねづのぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどぴちぴどぶづぜ〴ぜ〰ぐぃげぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: きちぴどのでぜ〴ぜ〰ちのつぜ〴ぜ〰こづねづのぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどぴちぴどぶづぜ〴ぜ〰ぐぃげぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ごどびびふづぜ〴ぜ〰こちねばぬづぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどぴちぴどぶづぜ〴ぜ〰ぐぃげぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: けふちのぴどぴちぴどぶづぜ〴ぜ〰ぐぃげぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰いどててづひづのぴどちぬぜ〴ぜ〰ぇづのづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰げちぢぢどぴぜ〴ぜ〰うづねちぬづぜ〴ぜ〰げづばひはつふっぴどぶづぜ〴ぜ〰ごひちっぴぜ〴ぜ〰〲〰ぜ〴ぜ〰とぜ〴ぜ〰ちてぴづひぜ〴ぜ〰ぎちぴふひちぬぜ〴ぜ〰きちぴどのでぜ〴ぜ〰はひぜ〴ぜ〰ざちでどのちぬぜ〴ぜ〰ぉのてふびどはのぜ〴ぜ〰しどぴとぜ〴ぜ〰こばづひね〭うひづづぜ〴ぜ〰こづねどのちぬぜ〴ぜ〰ぐぬちびねちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰いどててづひづのぴどちぬぜ〴ぜ〰ぇづのづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰げちぢぢどぴぜ〴ぜ〰うづねちぬづぜ〴ぜ〰げづばひはつふっぴどぶづぜ〴ぜ〰ごひちっぴぜ〴ぜ〰てひはねぜ〴ぜ〰くぶふぬちぴどはのぜ〴ぜ〰ぜ〵ぜ〰〱〰ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〵ㅜ〰ぜ〴ぜ〰ぴはぜ〴ぜ〰ふばぜ〴ぜ〰ぴはぜ〴ぜ〰〷〲ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぁあえい〲ぜ〴ぜ〰ちのつぜ〴ぜ〰ぃごぅぎぜ〴ぜ〰うはぬぬはぷぜ〴ぜ〰ちぜ〴ぜ〰こどねどぬちひぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ごどびびふづびぜ〴ぜ〰ちぴぜ〴ぜ〰〲〰ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぎぇうぜ〴ぜ〰うはぬぬはぷびぜ〴ぜ〰ちのぜ〴ぜ〰さば〭げづでふちぴどはのぜ〴ぜ〰しちぶづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ちぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴ぜ〰ごどねづ〭いづばづのつづのぴぜ〴ぜ〰きちののづひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰くぶふぬちぴづつぜ〴ぜ〰うはぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅねぢひべはぜ〴ぜ〰げづっはぶづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: いどててづひづのぴどちぬぜ〴ぜ〰ぇづのづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰げちぢぢどぴぜ〴ぜ〰うづねちぬづぜ〴ぜ〰げづばひはつふっぴどぶづぜ〴ぜ〰ごひちっぴぜ〴ぜ〰〲〰ぜ〴ぜ〰とぜ〴ぜ〰ちてぴづひぜ〴ぜ〰ぎちぴふひちぬぜ〴ぜ〰きちぴどのでぜ〴ぜ〰はひぜ〴ぜ〰ざちでどのちぬぜ〴ぜ〰ぉのてふびどはのぜ〴ぜ〰しどぴとぜ〴ぜ〰こばづひね〭うひづづぜ〴ぜ〰こづねどのちぬぜ〴ぜ〰ぐぬちびねちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰いどててづひづのぴどちぬぜ〴ぜ〰ぇづのづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰げちぢぢどぴぜ〴ぜ〰うづねちぬづぜ〴ぜ〰げづばひはつふっぴどぶづぜ〴ぜ〰ごひちっぴぜ〴ぜ〰てひはねぜ〴ぜ〰くぶふぬちぴどはのぜ〴ぜ〰ぜ〵ぜ〰〱〰ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〵ㅜ〰ぜ〴ぜ〰ぴはぜ〴ぜ〰ふばぜ〴ぜ〰ぴはぜ〴ぜ〰〷〲ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぁあえい〲ぜ〴ぜ〰ちのつぜ〴ぜ〰ぃごぅぎぜ〴ぜ〰うはぬぬはぷぜ〴ぜ〰ちぜ〴ぜ〰こどねどぬちひぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ごどびびふづびぜ〴ぜ〰ちぴぜ〴ぜ〰〲〰ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぎぇうぜ〴ぜ〰うはぬぬはぷびぜ〴ぜ〰ちのぜ〴ぜ〰さば〭げづでふちぴどはのぜ〴ぜ〰しちぶづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ちぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴ぜ〰ごどねづ〭いづばづのつづのぴぜ〴ぜ〰きちののづひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰くぶふぬちぴづつぜ〴ぜ〰うはぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅねぢひべはぜ〴ぜ〰げづっはぶづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどててづひづのぴどちぬぜ〴ぜ〰ぇづのづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぴとづぜ〴ぜ〰げちぢぢどぴぜ〴ぜ〰うづねちぬづぜ〴ぜ〰げづばひはつふっぴどぶづぜ〴ぜ〰ごひちっぴぜ〴ぜ〰てひはねぜ〴ぜ〰くぶふぬちぴどはのぜ〴ぜ〰ぜ〵ぜ〰〱〰ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〵ㅜ〰ぜ〴ぜ〰ぴはぜ〴ぜ〰ふばぜ〴ぜ〰ぴはぜ〴ぜ〰〷〲ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぁあえい〲ぜ〴ぜ〰ちのつぜ〴ぜ〰ぃごぅぎぜ〴ぜ〰うはぬぬはぷぜ〴ぜ〰ちぜ〴ぜ〰こどねどぬちひぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ごどびびふづびぜ〴ぜ〰ちぴぜ〴ぜ〰〲〰ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぎぇうぜ〴ぜ〰うはぬぬはぷびぜ〴ぜ〰ちのぜ〴ぜ〰さば〭げづでふちぴどはのぜ〴ぜ〰しちぶづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ちぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴ぜ〰ごどねづ〭いづばづのつづのぴぜ〴ぜ〰きちののづひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰くぶふぬちぴづつぜ〴ぜ〰うはぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅねぢひべはぜ〴ぜ〰げづっはぶづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぁあえい〲ぜ〴ぜ〰ちのつぜ〴ぜ〰ぃごぅぎぜ〴ぜ〰うはぬぬはぷぜ〴ぜ〰ちぜ〴ぜ〰こどねどぬちひぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ごどびびふづびぜ〴ぜ〰ちぴぜ〴ぜ〰〲〰ぜ〴ぜ〰とぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぎぇうぜ〴ぜ〰うはぬぬはぷびぜ〴ぜ〰ちのぜ〴ぜ〰さば〭げづでふちぴどはのぜ〴ぜ〰しちぶづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ちぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴ぜ〰ごどねづ〭いづばづのつづのぴぜ〴ぜ〰きちののづひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰くぶふぬちぴづつぜ〴ぜ〰うはぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅねぢひべはぜ〴ぜ〰げづっはぶづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぎぇうぜ〴ぜ〰うはぬぬはぷびぜ〴ぜ〰ちのぜ〴ぜ〰さば〭げづでふちぴどはのぜ〴ぜ〰しちぶづぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぐちぴぴづひのぜ〴ぜ〰どのぜ〴ぜ〰ちぜ〴ぜ〰ぐはびぴ〭きちぴどのでぜ〴ぜ〰ごどねづ〭いづばづのつづのぴぜ〴ぜ〰きちののづひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰くぶふぬちぴづつぜ〴ぜ〰うはぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅねぢひべはぜ〴ぜ〰げづっはぶづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: くぶふぬちぴづつぜ〴ぜ〰うはぬぬどっぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅねぢひべはぜ〴ぜ〰げづっはぶづひべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: IELMKZ+Rpxr FontSubset: true FirstChar: 11 LastChar: 61 FontDescriptor: FontName: IELMKZ+Rpxr Flags: Symbolic FontBBox: -40, -283, 904, 728 FontFile: true Font: BaseFont: VVSYYB+EURM10 FontSubset: true FirstChar: 11 LastChar: 22 FontDescriptor: FontName: VVSYYB+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: YICMPF+Rpcxr FontSubset: true FirstChar: 151 LastChar: 174 FontDescriptor: FontName: YICMPF+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: 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