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178 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:04:39 CEST RepresentationInformation: pmc_32708913.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:47:07 CEST Size: 1254987 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 429 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Sperm DNA Fragmentation Destination: subsection.2.1 Item: Title: Analysis of Differentially Expressed Proteins Using 2D-Differential Gel Electrophoresis (2D-DIGE) Destination: subsection.2.2 Item: Title: Identification of Differentially Expressed Proteins Using Mass Spectrometry (MS) Destination: subsection.2.3 Item: Title: Functional Classification of Protein and Protein-Protein Interaction Network Destination: subsection.2.4 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Semen Samples and Sperm DNA Fragmentation Destination: subsection.4.1 Children: Item: Title: Samples and Study Design Destination: subsubsection.4.1.1 Item: Title: Semen Collection, Basic Semen Analysis, and Cryopreservation Destination: subsubsection.4.1.2 Item: Title: DNA Integrity Tests: SCD, Alkaline Comet and Neutral Comet Destination: subsubsection.4.1.3 Item: Title: Proteomic Analysis of Seminal Plasma Destination: subsection.4.2 Children: Item: Title: Sample Preparation for 2-D DIGE Destination: subsubsection.4.2.1 Item: Title: Protein Labeling with CyDye DIGE Destination: subsubsection.4.2.2 Item: Title: Two-Dimensional Gel Electrophoresis Destination: subsubsection.4.2.3 Item: Title: Spot Picking and In-Gel Digestion for MS Analysis Destination: subsubsection.4.2.4 Item: Title: Protein Identification Destination: subsubsection.4.2.5 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