2990046a20102b6df6725e95cb68ed23 pmc_32823724.pdf c66461d7ab5ed916585eb34268f3799ac3ad1ddc pmc_32823724.pdf d1a9505c89eb26021ff67fe9555ed7bee406175696b4565d1a0c7bcfa9efc1ec pmc_32823724.pdf Title: UHPLC-HRMS and GC-MS Screening of a Selection of Synthetic Cannabinoids and Metabolites in Urine of Consumers Subject: Background and Objectives: The use of synthetic cannabinoids has increased around the world. As a result, the implementation of accurate analysis in human biological matrices is relevant and fundamental. Two different analytical technologies, ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and high-sensitivity gas chromatography-mass spectrometry (GC-MS) were used for the determination of three synthetic cannabinoids JWH-122, JWH 210, UR-144 and their metabolites in urine of consumers. Materials and Methods: Sample preparation included an initial hydrolysis with -glucuronidase and liquid-liquid extraction. The UHPLC-HRMS method included a Kinetex 2.6 u Biphenyl 100A (100 2.1 mm, 2.6 m) (Phenomenex, Italy) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode was used (mass range 100–1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m 250 m i.d, film thickness 0.25 m; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40–550 m/z). Results: Both methods have been successfully used for screening of parent synthetic cannabinoids and their metabolites in urine samples of consumers. Conclusions: The screening method applied JWH-122, JWH-210, UR-144 and their metabolites in urine of consumers can be applied to other compounds of the JWH family. Keywords: synthetic cannabinoids; urine; liquid chromatography; high-resolution mass spectrometry; gas chromatography-mass spectrometry Author: Manuela Pellegrini, Emilia Marchei, Esther Papaseit, Magí Farré and Simona Zaami Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Aug 13 13:45:22 2020 CEST ModDate: Thu Aug 13 13:45:22 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1105733 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YRCRBO+URWPalladioL-Ital Type 1 Custom yes yes no 66 0 EPDZBO+URWPalladioL-Bold Type 1 Custom yes yes no 67 0 QTLELQ+Pxbsy Type 1 Builtin yes yes no 68 0 YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 69 0 UFAPBI+URWPalladioL-Roma Type 1 Custom yes yes no 70 0 YBCNYQ+VnURWPalladioL Type 1 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no 204 0 MFIGJL+Rpxb Type 1 Builtin yes yes no 279 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 326 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-11-18 06:12:03 CET RepresentationInformation: pmc_32823724.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-09-21 03:45:51 CEST Size: 1105733 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きづぴとはつはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰こちねばぬづぜ〴ぜ〰ぐひづばちひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぇちびぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭きちびびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰ぇぃ〭きこぜ〵ㅜ〰ぜ〴ぜ〰ぉのびぴひふねづのぴちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰さぬぴひち〭えどでと〭ぐづひてはひねちのっづぜ〴ぜ〰がどぱふどつぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭えどでと〭げづびはぬふぴどはのぜ〴ぜ〰ぁっっふひちぴづぜ〴ぜ〰きちびびづびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰さえぐがぃ〭えげきこぜ〵ㅜ〰ぜ〴ぜ〰ぉのびぴひふねづのぴちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ことはひぴぜ〴ぜ〰きづぴとはつびぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: こちねばぬづぜ〴ぜ〰ぐひづばちひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぇちびぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭きちびびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰ぇぃ〭きこぜ〵ㅜ〰ぜ〴ぜ〰ぉのびぴひふねづのぴちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰さぬぴひち〭えどでと〭ぐづひてはひねちのっづぜ〴ぜ〰がどぱふどつぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭えどでと〭げづびはぬふぴどはのぜ〴ぜ〰ぁっっふひちぴづぜ〴ぜ〰きちびびづびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰さえぐがぃ〭えげきこぜ〵ㅜ〰ぜ〴ぜ〰ぉのびぴひふねづのぴちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ことはひぴぜ〴ぜ〰きづぴとはつびぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぇちびぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭きちびびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰ぇぃ〭きこぜ〵ㅜ〰ぜ〴ぜ〰ぉのびぴひふねづのぴちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰さぬぴひち〭えどでと〭ぐづひてはひねちのっづぜ〴ぜ〰がどぱふどつぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭えどでと〭げづびはぬふぴどはのぜ〴ぜ〰ぁっっふひちぴづぜ〴ぜ〰きちびびづびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰さえぐがぃ〭えげきこぜ〵ㅜ〰ぜ〴ぜ〰ぉのびぴひふねづのぴちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ことはひぴぜ〴ぜ〰きづぴとはつびぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: さぬぴひち〭えどでと〭ぐづひてはひねちのっづぜ〴ぜ〰がどぱふどつぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭えどでと〭げづびはぬふぴどはのぜ〴ぜ〰ぁっっふひちぴづぜ〴ぜ〰きちびびづびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰さえぐがぃ〭えげきこぜ〵ㅜ〰ぜ〴ぜ〰ぉのびぴひふねづのぴちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ことはひぴぜ〴ぜ〰きづぴとはつびぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ことはひぴぜ〴ぜ〰きづぴとはつびぜ〴ぜ〰ざちぬどつちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: YRCRBO+URWPalladioL-Ital FontSubset: true FirstChar: 40 LastChar: 121 FontDescriptor: FontName: YRCRBO+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: 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