b1e89af01f70341daa6987dc047b7b00 pmc_32825637.pdf 21960022eed93be69a4011cd7c36ee608309d0bc pmc_32825637.pdf 19432164b05d6fcfac42e256bbf0aa3879661792c53aaf9a45475db140468af8 pmc_32825637.pdf Title: Remodeling of Kv7.1 and Kv7.5 Expression in Vascular Tumors Subject: Voltage-dependent potassium (Kv) channels contribute to the excitability of nerves and muscles. In addition, Kv participates in several cell functions, including cell cycle progression and proliferation. Kv channel remodeling has been associated with neoplastic cell growth and cancer. Kv7 channels are expressed in blood vessels, and they participate in the maintenance of vascular tone and are implicated in myocyte proliferation. Although evidence links Kv7 remodeling to different types of cancer, its expression in vascular tumors has never been studied. Endothelium-derived vascular neoplasms range from indolent lesions to highly aggressive and metastasizing cancers. Here, we show that Kv7.1 and Kv7.5 are evenly distributed in tunicas as well as the endothelium of healthy veins and arteries. The layered structure of vessels is lost in vascular tumors. By studying eight vascular tumors with different origins and characteristics, we found that Kv7.1 and Kv7.5 expression was changed in vascular cancers. While both channels were generally downregulated, Kv7.5 expression was clearly correlated with neoplastic malignancy. The vascular tumors did not contract; therefore, the role of Kv7 channels is probably related to proliferation rather than controlling vascular tone. Our results identify vascular Kv7 channels as targets for cancer detection and anticancer therapies. Keywords: potassium channels; blood vessels; neoplasia; cancer Author: Clara Serrano-Novillo, Anna Oliveras, Joan Carles Ferreres, Enric Condom and Antonio Felipe Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Aug 21 11:12:23 2020 CEST ModDate: Fri Aug 21 11:12:23 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2904715 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- GPMQJV+URWPalladioL-Ital Type 1 Custom yes yes no 75 0 SKBGIF+URWPalladioL-Bold Type 1 Custom yes yes no 76 0 GOLWXN+URWPalladioL-Roma Type 1 Custom yes yes no 77 0 BZUWYZ+VnURWPalladioL Type 1 Custom yes yes no 78 0 OFLXAT+Rpxr Type 1 Builtin yes yes no 79 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 84 0 IRWHZX+TimesNewRomanPSMT Type 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げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぅへばひづびびどはのぜ〴ぜ〰はてぜ〴ぜ〰かぶ〷〮〱ぜ〴ぜ〰ちのつぜ〴ぜ〰かぶ〷〮〵ぜ〴ぜ〰どのぜ〴ぜ〰えふねちのぜ〴ぜ〰あぬははつぜ〴ぜ〰ざづびびづぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰かぶぜ〴ぜ〰ぃとちののづぬぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ざちびっふぬちひぜ〴ぜ〰ごふねはひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰かぶ〷〮〱ぜ〴ぜ〰ちのつぜ〴ぜ〰かぶ〷〮〵ぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぃはひひづぬちぴづびぜ〴ぜ〰ぷどぴとぜ〴ぜ〰ごふねはひぜ〴ぜ〰きちぬどでのちのっべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぅへばひづびびどはのぜ〴ぜ〰はてぜ〴ぜ〰かぶ〷〮〱ぜ〴ぜ〰ちのつぜ〴ぜ〰かぶ〷〮〵ぜ〴ぜ〰どのぜ〴ぜ〰えふねちのぜ〴ぜ〰あぬははつぜ〴ぜ〰ざづびびづぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰かぶぜ〴ぜ〰ぃとちののづぬぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ざちびっふぬちひぜ〴ぜ〰ごふねはひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰かぶ〷〮〱ぜ〴ぜ〰ちのつぜ〴ぜ〰かぶ〷〮〵ぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぃはひひづぬちぴづびぜ〴ぜ〰ぷどぴとぜ〴ぜ〰ごふねはひぜ〴ぜ〰きちぬどでのちのっべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: かぶぜ〴ぜ〰ぃとちののづぬぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ざちびっふぬちひぜ〴ぜ〰ごふねはひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰かぶ〷〮〱ぜ〴ぜ〰ちのつぜ〴ぜ〰かぶ〷〮〵ぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぃはひひづぬちぴづびぜ〴ぜ〰ぷどぴとぜ〴ぜ〰ごふねはひぜ〴ぜ〰きちぬどでのちのっべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: かぶ〷〮〱ぜ〴ぜ〰ちのつぜ〴ぜ〰かぶ〷〮〵ぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰ぃはひひづぬちぴづびぜ〴ぜ〰ぷどぴとぜ〴ぜ〰ごふねはひぜ〴ぜ〰きちぬどでのちのっべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮ㄩ‾㸊⡜㌷㙜㌷㝜〰ごどびびふづぜ〴ぜ〰こちねばぬづび〬ぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぅへぴひちっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰うぬふはひづびっづのっづぜ〴ぜ〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰どのぜ〴ぜ〰えふねちのぜ〴ぜ〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㌩‾㸊⡜㌷㙜㌷㝜〰ぃはのてはっちぬぜ〴ぜ〰こっちののどのでぜ〴ぜ〰がちびづひぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰えどびぴはぬはでどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Children: Item: Title: ごどびびふづぜ〴ぜ〰こちねばぬづび〬ぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぅへぴひちっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰しづびぴづひのぜ〴ぜ〰あぬはぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰うぬふはひづびっづのっづぜ〴ぜ〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰どのぜ〴ぜ〰えふねちのぜ〴ぜ〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㌩‾㸊⡜㌷㙜㌷㝜〰ぃはのてはっちぬぜ〴ぜ〰こっちののどのでぜ〴ぜ〰がちびづひぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰えどびぴはぬはでどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: うぬふはひづびっづのっづぜ〴ぜ〰ぉねねふのはとどびぴはっとづねどびぴひべぜ〴ぜ〰どのぜ〴ぜ〰えふねちのぜ〴ぜ〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㌩‾㸊⡜㌷㙜㌷㝜〰ぃはのてはっちぬぜ〴ぜ〰こっちののどのでぜ〴ぜ〰がちびづひぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰えどびぴはぬはでどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: 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Annotation: Subtype: Link Rect: 331, 256, 367, 267 Annotation: Subtype: Link Rect: 480, 230, 516, 241 Annotation: Subtype: Link Rect: 100, 193, 137, 203 Annotation: Subtype: Link Rect: 451, 166, 488, 177 Annotation: Subtype: Link Rect: 100, 115, 137, 125 Annotation: Subtype: Link Rect: 249, 89, 287, 99 Annotation: Subtype: Link Rect: 293, 89, 329, 99 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 291, 728, 329, 738 Annotation: Subtype: Link Rect: 335, 728, 371, 738 Annotation: Subtype: Link Rect: 389, 701, 426, 712 Annotation: Subtype: Link Rect: 150, 676, 187, 686 Annotation: Subtype: Link Rect: 193, 676, 229, 686 Annotation: Subtype: Link Rect: 100, 625, 137, 635 Annotation: Subtype: Link Rect: 143, 625, 179, 635 Annotation: Subtype: Link Rect: 345, 599, 383, 609 Annotation: Subtype: Link Rect: 76, 557, 166, 588 Annotation: Subtype: Link Rect: 234, 551, 407, 562 Checksum: 52c9c7a5 Type: CRC32 Checksum: b1e89af01f70341daa6987dc047b7b00 Type: MD5 Checksum: 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