1f9270fb4b6d3aea82afa3146ed2df69 pmc_32850799.pdf cd76dbf198f886088c9c172e74b93662e39dc143 pmc_32850799.pdf d74dc7e245824533e6417f8d1fca4574f4345bb43cae0726dda0f6ed4349f2d8 pmc_32850799.pdf Title: Mitochondrial Proteome of Affected Glutamatergic Neurons in a Mouse Model of Leigh Syndrome Subject: Defects in mitochondrial function lead to severe neuromuscular orphan pathologies known as mitochondrial disease. Keywords: leigh syndrome, animal models, neuroscience, proteomics, cell type-specific, mitochondrial isolation Author: Elisenda Sanz and Albert Quintana Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Sat Jul 25 14:36:54 2020 CEST ModDate: Sat Jul 25 14:36:54 2020 CEST Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 3027505 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JJNFUE+MinionPro-Regular Type 1 Custom yes yes no 32 0 OIWGUC+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 33 0 PCZSME+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 34 0 CCVXXJ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 39 0 YTJDEM+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 40 0 TADMKV+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 41 0 QPJXZF+MTGU Type 1 Builtin yes yes no 42 0 BUBUNP+MinionPro-It Type 1 Custom yes yes no 114 0 IXMZZG+MTSYN Type 1 Builtin yes yes no 122 0 XQHBTT+RBLMI Type 1 Builtin yes yes no 126 0 AYIBMK+MinionPro-Bold Type 1 Custom yes yes no 297 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:02:31 CEST RepresentationInformation: pmc_32850799.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:47:19 CEST Size: 3027505 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 820 FreeObjects: 4 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Mitochondrial Proteome of Affected Glutamatergic Neurons in a Mouse Model of Leigh Syndrome Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Animal Husbandry Item: Title: Mouse Genetics Item: Title: Cell Cultures Item: Title: Immunofluorescence Item: Title: Western Blotting Item: Title: Double-Labeled in situ Hybridization (ISH)/ Immunofluorescence (IF) Item: Title: Transmission Electron Microscopy Item: Title: MitoTag Adeno-Associated Viral Vector (AAV) Generation Item: Title: AAV-mitoTag Delivery Item: Title: Isolation of Mitochondria by Immunoaffinity Purification Item: Title: Isolation of Mitochondria by Double Centrifugation Item: Title: Pyruvate Dehydrogenase (PDH) Activity Item: Title: Measurement of Respiration in Isolated Mitochondria Item: Title: Protein Extraction and Digestion Item: Title: Peptide Labeling by Isobaric Tandem Mass Tag Item: Title: Acetylated Peptide Enrichment Item: Title: LC MSn Analysis Item: Title: Database Search and Proteomic Data Analysis Item: Title: Bioinformatics Analysis Item: Title: Statistics Item: Title: Results Children: Item: Title: Generation and Validation of the AAV-mitoTag Viral Vector Item: Title: Cell Type-Specific Isolation of Mitochondria From Glutamatergic Neurons in the VN Item: Title: Identification and Quantitation of the Global and Acetyl-Proteome in Mitochondria From Glutamatergic Neurons in the VN Item: Title: Hyperacetylation of PDH and Complex V Subunits in VN Neurons of Vglut2:Ndufs4cKO Mice Item: Title: Discussion Item: Title: Data Availability Statement Item: Title: Ethics Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: Mitochondrial Proteome of Affected Glutamatergic Neurons in a Mouse Model of Leigh Syndrome Author: Elisenda Sanz and Albert Quintana Subject: Defects in mitochondrial function lead to severe neuromuscular orphan pathologies known as mitochondrial disease. 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551, 225 Annotation: Subtype: Link Rect: 312, 206, 396, 215 Annotation: Subtype: Link Rect: 414, 189, 511, 196 Annotation: Subtype: Link Rect: 405, 168, 496, 178 Annotation: Subtype: Link Rect: 473, 140, 551, 149 Annotation: Subtype: Link Rect: 312, 132, 334, 139 Annotation: Subtype: Link Rect: 446, 102, 543, 111 Annotation: Subtype: Link Rect: 425, 64, 527, 73 Annotation: Subtype: Link Rect: 43, 29, 176, 38 Annotation: Subtype: Link Rect: 179, 29, 239, 38 Annotation: Subtype: Link Rect: 449, 29, 551, 38 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 235, 688, 293, 697 Annotation: Subtype: Link Rect: 53, 679, 84, 687 Annotation: Subtype: Link Rect: 240, 631, 293, 640 Annotation: Subtype: Link Rect: 53, 623, 96, 630 Annotation: Subtype: Link Rect: 206, 593, 293, 602 Annotation: Subtype: Link Rect: 53, 585, 75, 592 Annotation: Subtype: Link Rect: 178, 528, 267, 536 Annotation: Subtype: Link Rect: 174, 489, 271, 498 Annotation: Subtype: Link Rect: 156, 432, 219, 441 Annotation: Subtype: Link Rect: 243, 375, 293, 384 Annotation: Subtype: Link Rect: 53, 367, 73, 374 Annotation: Subtype: Link Rect: 202, 337, 293, 347 Annotation: Subtype: Link Rect: 53, 330, 91, 336 Annotation: Subtype: Link Rect: 137, 299, 230, 309 Annotation: Subtype: Link Rect: 98, 262, 191, 271 Annotation: Subtype: Link Rect: 268, 233, 293, 243 Annotation: Subtype: Link Rect: 53, 225, 97, 233 Annotation: Subtype: Link Rect: 149, 195, 215, 205 Annotation: Subtype: Link Rect: 204, 138, 293, 148 Annotation: Subtype: Link Rect: 53, 131, 75, 138 Annotation: Subtype: Link Rect: 508, 706, 551, 716 Annotation: Subtype: Link Rect: 312, 697, 384, 706 Annotation: Subtype: Link Rect: 537, 659, 551, 668 Annotation: Subtype: Link Rect: 312, 650, 389, 659 Annotation: Subtype: Link Rect: 526, 631, 551, 640 Annotation: Subtype: Link Rect: 312, 621, 397, 631 Annotation: Subtype: Link Rect: 480, 602, 551, 612 Annotation: Subtype: Link Rect: 312, 595, 334, 601 Annotation: Subtype: Link Rect: 526, 564, 551, 574 Annotation: Subtype: Link Rect: 312, 557, 397, 564 Annotation: Subtype: Link Rect: 537, 527, 551, 536 Annotation: Subtype: Link Rect: 312, 517, 410, 526 Annotation: Subtype: Link Rect: 434, 498, 512, 508 Annotation: Subtype: Link Rect: 537, 470, 551, 479 Annotation: Subtype: Link Rect: 312, 460, 401, 470 Annotation: Subtype: Link Rect: 350, 434, 439, 441 Annotation: Subtype: Link Rect: 375, 394, 457, 403 Annotation: Subtype: Link Rect: 359, 366, 436, 375 Annotation: Subtype: Link Rect: 312, 292, 389, 299 Annotation: Subtype: Link Rect: 430, 224, 532, 233 Annotation: Subtype: Link Rect: 323, 148, 349, 157 Annotation: Subtype: Link Rect: 350, 148, 382, 157 Annotation: Subtype: Link Rect: 384, 148, 419, 157 Annotation: Subtype: Link Rect: 420, 148, 443, 157 Annotation: Subtype: Link Rect: 445, 148, 473, 157 Annotation: Subtype: Link Rect: 43, 29, 176, 38 Annotation: Subtype: Link Rect: 179, 29, 239, 38 Annotation: Subtype: Link Rect: 449, 29, 551, 38 Checksum: 86cf489c Type: CRC32 Checksum: 1f9270fb4b6d3aea82afa3146ed2df69 Type: MD5 Checksum: cd76dbf198f886088c9c172e74b93662e39dc143 Type: SHA-1 Checksum: d74dc7e245824533e6417f8d1fca4574f4345bb43cae0726dda0f6ed4349f2d8 Type: SHA-256