d06b2c91279dead1cfb1a60bed991cfd animals_a2021v11n1p88.pdf 888f033dc7651214c04f9d0d4b76da7e44d55ce0 animals_a2021v11n1p88.pdf 1e9e2d080f1ec0a2a4795d5701d518fb348357dfdc64579476fc72c21fc3639a animals_a2021v11n1p88.pdf Title: Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing Subject: Endometritis is associated with fertility problems in many species, with endometrial biopsy being the main diagnostic tool. In feline queens, the reduced size of the uterus may make it difficult to obtain representative diagnostic samples. Endometrial cytology may represent a valuable diagnostic tool for evaluating the health status of the endometrium in queens. Fifty domestic shorthair queens were included and divided into two cytological diagnostic technique groups, the uterine lavage (UL; n = 28) and uterine swabbing (US; n = 22) groups. Cytological results were compared with histopathological and bacteriological information. Changes in the histopathological patterns were also evaluated and compared with progesterone levels to confirm previous published data. Furthermore, the results from both cytological sampling methods were compared to evaluate the utility of each method. Endometritis was ruled out in all queens by means of histology and microbiology. Leukocyte counts and red blood cell/endometrial cell ratios were significantly higher in US than UL samples. Additionally, UL sampling is less affected by blood contamination and cells are better preserved. The combination of endometrial cytology and uterine culture might be useful for evaluating the endometrial characteristics in queens. The UL evaluation method is more representative of the actual endometrial status than the US technique. Keywords: uterus; cytology; polymorphonuclear neutrophil; queen Author: Alba Martí, Anna Serrano, Josep Pastor, Teresa Rigau, Ugné Petkevičiuté, Maria Àngels Calvo, Esteban Leonardo Arosemena, Aida Yuste, David Prandi, Adrià Aguilar and Maria Montserrat Rivera del Alamo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Jan 14 02:49:53 2021 CET ModDate: Thu Jan 14 08:38:57 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 6147601 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MZZAJO+VnURWPalladioL Type 1 Custom yes yes no 10 0 ZLDWOP+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 PBHZJR+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 TRUUOP+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 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no 319 0 RFQKST+VnURWPalladioL-Italic Type 1 Custom yes yes no 333 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:56:01 CEST RepresentationInformation: animals_a2021v11n1p88.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:50:22 CEST Size: 6147601 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 495 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals Destination: subsection.2.1 Item: Title: Sample Collection Destination: subsection.2.2 Item: Title: Progesterone Destination: subsection.2.3 Item: Title: Microbiology Destination: subsection.2.4 Item: Title: Histopathology Destination: subsection.2.5 Item: Title: Cytology Samples Destination: subsection.2.6 Children: Item: Title: Smear Quality Destination: subsubsection.2.6.1 Item: Title: Cellularity and Cell Morphology Destination: subsubsection.2.6.2 Item: Title: Cell Ratios Destination: subsubsection.2.6.3 Item: Title: Inflammation Destination: subsubsection.2.6.4 Item: Title: Statistical Analysis Destination: subsection.2.7 Item: Title: Results Destination: section.3 Children: Item: Title: Progesterone Destination: subsection.3.1 Item: Title: Microbiology Destination: subsection.3.2 Item: Title: Histology Destination: subsection.3.3 Item: Title: Cytology Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing Author: ぁぬぢち〠きちひぴロ〬〠ぁののち〠こづひひちのは〬〠おはびづば〠ぐちびぴはひ〬〠ごづひづびち〠げどでちふ〬〠さでのラ〠ぐづぴにづぶどㄍどふぴラ〬〠きちひどち〠ダのでづぬび〠ぃちぬぶは〬〠ぅびぴづぢちの〠がづはのちひつは〠ぁひはびづねづのち〬〠ぁどつち〠すふびぴづ〬〠いちぶどつ〠ぐひちのつど〬〠ぁつひどム〠ぁでふどぬちひ〠ちのつ〠きちひどち〠きはのぴびづひひちぴ〠げどぶづひち〠つづぬ〠ぁぬちねは Subject: Endometritis is associated with fertility problems in many species, with endometrial biopsy being the main diagnostic tool. In feline queens, the reduced size of the uterus may make it difficult to obtain representative diagnostic samples. Endometrial cytology may represent a valuable diagnostic tool for evaluating the health status of the endometrium in queens. Fifty domestic shorthair queens were included and divided into two cytological diagnostic technique groups, the uterine lavage (UL; n = 28) and uterine swabbing (US; n = 22) groups. Cytological results were compared with histopathological and bacteriological information. Changes in the histopathological patterns were also evaluated and compared with progesterone levels to confirm previous published data. Furthermore, the results from both cytological sampling methods were compared to evaluate the utility of each method. Endometritis was ruled out in all queens by means of histology and microbiology. Leukocyte counts and red blood cell/endometrial cell ratios were significantly higher in US than UL samples. Additionally, UL sampling is less affected by blood contamination and cells are better preserved. The combination of endometrial cytology and uterine culture might be useful for evaluating the endometrial characteristics in queens. The UL evaluation method is more representative of the actual endometrial status than the US technique. 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