aaf4508c475ca104a20be5b73947a05c ijenvrespubhea_a2021v18n3p1305.pdf 4af6925bffafc7f7ab2cf6f324de96498e4c05dc ijenvrespubhea_a2021v18n3p1305.pdf c6b34b9624903a394e2986a9c2707e9eaa39f6415ba2cdb60148ee308949e249 ijenvrespubhea_a2021v18n3p1305.pdf Title: Microbial Diversity Dynamics in a Methanogenic-Sulfidogenic UASB Reactor Subject: In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42-L-1 and a constant C/S ratio of 1.5 0.3 g Cg-1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB. Keywords: bioscrubber; crude glycerol; UASB; sulfate-reducing bacteria; microbial diversity shifts; sequencing Author: E. Fernández-Palacios, Xudong Zhou, Mabel Mora and David Gabriel Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Feb 1 14:04:33 2021 CET ModDate: Mon Feb 1 14:18:01 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3355286 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- VBHBAG+URWPalladioL-Ital Type 1 Custom yes yes no 10 0 NUTKGN+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 FEZQEH+URWPalladioL-Roma Type 1 Custom yes yes no 19 0 XVSDDF+CMSY10 Type 1 Builtin yes yes no 23 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 27 0 RAFMMA+VnURWPalladioL Type 1 Custom yes yes no 32 0 LWQXII+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes 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2024-10-02 03:55:29 CEST RepresentationInformation: ijenvrespubhea_a2021v18n3p1305.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:50:26 CEST Size: 3355286 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 629 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Reactor Set-Up and Operation Destination: subsection.2.1 Item: Title: Analytical Methods Destination: subsection.2.2 Item: Title: Illumina Sequencing Analysis Destination: subsection.2.3 Item: Title: Results Destination: section.3 Children: Item: Title: Long-Term Performance of the UASB at Constant Loading Rate Destination: subsection.3.1 Item: Title: Microbial Diversity Changes and Stratification in the Sulfidogenic UASB Destination: subsection.3.2 Children: Item: Title: Long-Term Performance and Microbial Community Analysis Destination: subsubsection.3.2.1 Item: Title: Chemical and Microbial Stratification in the UASB Reactor under Non-Methanogenic Conditions Destination: subsubsection.3.2.2 Item: Title: Discussion Destination: section.4 Children: Item: Title: Long-Term Performance and Microbial Community Analyses Destination: subsection.4.1 Item: Title: Stratification of Microbial Communities and Chemicals Destination: subsection.4.2 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Microbial Diversity Dynamics in a Methanogenic-Sulfidogenic UASB Reactor Author: E. Fernández-Palacios, Xudong Zhou, Mabel Mora and David Gabriel Subject: In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42-L-1 and a constant C/S ratio of 1.5 0.3 g Cg-1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB. 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