61cbb8502a016d4b7c2187c214635425 nutrients_a2021v13n2p636.pdf ca720d1e9beb64771bdfc8f3043350ef72608dfb nutrients_a2021v13n2p636.pdf 5fed767cb8fcb7e690967536a537a1520fc12f6b3b96bccb820c1701cbeb6dd5 nutrients_a2021v13n2p636.pdf Title: Gut Microbiota Bacterial Species Associated with Mediterranean Diet-Related Food Groups in a Northern Spanish Population Subject: The MD (Mediterranean diet) is recognized as one of the healthiest diets worldwide and is associated with the prevention of cardiovascular and metabolic diseases. Dietary habits are considered one of the strongest modulators of gut microbiota, which seem to play a significant role in health status of the host. The purpose of the present study was to evaluate interactive associations between gut microbiota composition and habitual dietary intake in 360 Spanish adults from the Obekit cohort (normal weight, overweight, and obese participants). Dietary intake and adherence to the MD tests were administered and fecal samples were collected from each participant. Fecal 16S rRNA (ribosomal Ribonucleic Acid) gene sequencing was performed and checked against the dietary habits. MetagenomeSeq was the statistical tool applied to analyze data at the species taxonomic level. Results from this study identified several beneficial bacteria that were more abundant in the individuals with higher adherence to the MD. Bifidobacterium animalis was the species with the strongest association with the MD. Some SCFA (Short Chain Fatty Acids) -producing bacteria were also associated with MD. In conclusion, this study showed that MD, fiber, legumes, vegetable, fruit, and nut intake are associated with an increase in butyrate-producing taxa such as Roseburia faecis, Ruminococcus bromii, and Oscillospira (Flavonifractor) plautii. Keywords: Bifidobacterium animalis; gut microbiota; short-chain fatty acids; obesity; butyrate Author: Carles Rosés, Amanda Cuevas-Sierra, Salvador Quintana, José I. Riezu-Boj, J. Alfredo Martínez, Fermín I. Milagro and Anna Barceló Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Feb 19 03:40:55 2021 CET ModDate: Fri Feb 19 03:43:27 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3261515 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OHKNFJ+VnURWPalladioL Type 1 Custom yes yes no 10 0 CNKFKA+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 QMYHJB+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 FHUYFU+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 YFFKLH+CMSY10 Type 1 Builtin yes yes no 78 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 88 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 95 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 110 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 114 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 118 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 122 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 144 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 148 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 152 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 156 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 173 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 177 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 181 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 185 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 192 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 213 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 217 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 221 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 225 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 232 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 249 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 253 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 257 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 261 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 268 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 279 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 283 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 287 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 291 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 298 0 LDHCMG+VnURWPalladioL-Italic Type 1 Custom yes yes no 327 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:54:37 CEST RepresentationInformation: nutrients_a2021v13n2p636.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:50:30 CEST Size: 3261515 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 564 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Material and Methods Destination: section.2 Children: Item: Title: Participants Destination: subsection.2.1 Item: Title: Anthropometric and Biochemical Measurements Destination: subsection.2.2 Item: Title: Dietary Estimation Destination: subsection.2.3 Item: Title: Faecal Sample Collection and DNA Extraction Destination: subsection.2.4 Item: Title: Metagenomic Data: Library Preparation Destination: subsection.2.5 Item: Title: Metagenomics Data: Analysis and Processing Destination: subsection.2.6 Item: Title: Richness and Evenness Destination: subsection.2.7 Item: Title: Statistical Analysis Destination: subsection.2.8 Item: Title: Prediction of Functional Potential of Gut Microbiota Destination: subsection.2.9 Item: Title: Results Destination: section.3 Children: Item: Title: Participant Characteristics Destination: subsection.3.1 Item: Title: Microbiota Composition: MD Adherence Destination: subsection.3.2 Item: Title: Microbiota Composition: Fibre Intake Destination: subsection.3.3 Item: Title: Microbiota Composition: Food Groups Destination: subsection.3.4 Item: Title: Species Richness, Evenness, and Diversity Destination: subsection.3.5 Item: Title: Comparison of Functional Potential of the Gut Microbiota Destination: subsection.3.6 Item: Title: Discussion Destination: section.4 Item: Title: MD High-Adherence Species Destination: section.5 Item: Title: Study Limitations Destination: section.6 Item: Title: Conclusions Destination: section.7 Item: Title: References Destination: section.7 Info: Title: Gut Microbiota Bacterial Species Associated with Mediterranean Diet-Related Food Groups in a Northern Spanish Population Author: Carles Rosés, Amanda Cuevas-Sierra, Salvador Quintana, José I. Riezu-Boj, J. Alfredo Martínez, Fermín I. Milagro and Anna Barceló Subject: The MD (Mediterranean diet) is recognized as one of the healthiest diets worldwide and is associated with the prevention of cardiovascular and metabolic diseases. Dietary habits are considered one of the strongest modulators of gut microbiota, which seem to play a significant role in health status of the host. The purpose of the present study was to evaluate interactive associations between gut microbiota composition and habitual dietary intake in 360 Spanish adults from the Obekit cohort (normal weight, overweight, and obese participants). Dietary intake and adherence to the MD tests were administered and fecal samples were collected from each participant. Fecal 16S rRNA (ribosomal Ribonucleic Acid) gene sequencing was performed and checked against the dietary habits. MetagenomeSeq was the statistical tool applied to analyze data at the species taxonomic level. Results from this study identified several beneficial bacteria that were more abundant in the individuals with higher adherence to the MD. Bifidobacterium animalis was the species with the strongest association with the MD. Some SCFA (Short Chain Fatty Acids) -producing bacteria were also associated with MD. In conclusion, this study showed that MD, fiber, legumes, vegetable, fruit, and nut intake are associated with an increase in butyrate-producing taxa such as Roseburia faecis, Ruminococcus bromii, and Oscillospira (Flavonifractor) plautii. Keywords: Bifidobacterium animalis; gut microbiota; short-chain fatty acids; obesity; butyrate Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Feb 19 03:40:55 CET 2021 ModDate: Fri Feb 19 03:43:27 CET 2021 ID: 0x9b83a4cbb2ba74444c8292b537fa474c, 0x9b83a4cbb2ba74444c8292b537fa474c Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QMYHJB+URWPalladioL-Bold FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: QMYHJB+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: LDHCMG+VnURWPalladioL-Italic FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: LDHCMG+VnURWPalladioL-Italic Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: FHUYFU+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: FHUYFU+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: OVNEXW+EURM10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: OVNEXW+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: OHKNFJ+VnURWPalladioL FontSubset: true FirstChar: 129 LastChar: 226 FontDescriptor: FontName: OHKNFJ+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XWOCXD+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 226 FontDescriptor: FontName: XWOCXD+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: YFFKLH+CMSY10 FontSubset: true FirstChar: 0 LastChar: 48 FontDescriptor: FontName: YFFKLH+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: CNKFKA+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: CNKFKA+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: QLBIEB+URWPalladioL-BoldItal FontSubset: true FirstChar: 110 LastChar: 112 FontDescriptor: FontName: QLBIEB+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 35, 759, 169, 795 Annotation: Subtype: Link Rect: 515, 759, 560, 795 Annotation: Subtype: Link Rect: 99, 641, 110, 654 Annotation: Subtype: Link Rect: 225, 641, 236, 654 Annotation: Subtype: Link Rect: 425, 641, 436, 654 Annotation: Subtype: Link Rect: 547, 641, 559, 654 Annotation: Subtype: Link Rect: 135, 628, 146, 642 Annotation: Subtype: Link Rect: 110, 334, 154, 343 Annotation: Subtype: Link Rect: 35, 322, 126, 331 Annotation: Subtype: Link Rect: 35, 162, 97, 184 Annotation: Subtype: Link Rect: 153, 89, 154, 98 Annotation: Subtype: Link Rect: 35, 77, 147, 86 Annotation: Subtype: Link Rect: 512, 206, 519, 215 ActionDest: 321 Annotation: Subtype: Link Rect: 237, 131, 244, 140 ActionDest: 321 Annotation: Subtype: Link Rect: 222, 106, 229, 115 ActionDest: 321 Annotation: Subtype: Link Rect: 117, 17, 251, 28 Annotation: Subtype: Link Rect: 402, 17, 558, 28 Annotation: Subtype: Link Rect: 35, 434, 87, 453 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 221, 737, 228, 746 ActionDest: 321 Annotation: Subtype: Link Rect: 228, 711, 235, 721 ActionDest: 321 Annotation: Subtype: Link Rect: 236, 711, 243, 721 ActionDest: 321 Annotation: Subtype: Link Rect: 210, 686, 217, 695 ActionDest: 321 Annotation: Subtype: Link Rect: 528, 649, 535, 658 ActionDest: 321 Annotation: Subtype: Link Rect: 510, 636, 517, 645 ActionDest: 321 Annotation: Subtype: Link Rect: 487, 573, 494, 582 ActionDest: 321 Annotation: Subtype: Link Rect: 494, 573, 506, 582 ActionDest: 321 Annotation: Subtype: Link Rect: 531, 536, 543, 545 ActionDest: 321 Annotation: Subtype: Link Rect: 433, 498, 445, 507 ActionDest: 321 Annotation: Subtype: Link Rect: 544, 473, 556, 482 ActionDest: 321 Annotation: Subtype: Link Rect: 522, 448, 534, 457 ActionDest: 321 Annotation: Subtype: Link Rect: 476, 423, 487, 432 ActionDest: 321 Annotation: Subtype: Link Rect: 205, 398, 217, 407 ActionDest: 321 Annotation: Subtype: Link Rect: 468, 372, 480, 382 ActionDest: 324 Annotation: Subtype: Link Rect: 376, 146, 388, 154 ActionDest: 324 Annotation: Subtype: Link Rect: 279, 95, 291, 104 ActionDest: 324 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 517, 672, 529, 681 ActionDest: 324 Annotation: Subtype: Link Rect: 522, 445, 534, 455 ActionDest: 324 Annotation: Subtype: Link Rect: 255, 383, 267, 392 ActionDest: 324 Annotation: Subtype: Link Rect: 522, 320, 534, 329 ActionDest: 324 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 262, 496, 274, 505 ActionDest: 324 Annotation: Subtype: Link Rect: 520, 408, 532, 417 ActionDest: 324 Annotation: Subtype: Link Rect: 480, 370, 492, 380 ActionDest: 324 Annotation: Subtype: Link Rect: 492, 370, 504, 380 ActionDest: 324 Annotation: Subtype: Link Rect: 327, 333, 339, 342 ActionDest: 324 Annotation: Subtype: Link Rect: 339, 333, 351, 342 ActionDest: 324 Annotation: Subtype: Link Rect: 489, 195, 501, 204 ActionDest: 324 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 318, 672, 330, 681 ActionDest: 324 Annotation: Subtype: Link Rect: 511, 596, 523, 605 ActionDest: 324 Annotation: Subtype: Link Rect: 359, 419, 371, 428 ActionDest: 324 Annotation: Subtype: Link Rect: 467, 417, 474, 429 ActionDest: 92 Annotation: Subtype: Link Rect: 227, 72, 234, 84 ActionDest: 92 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 272, 691, 279, 703 ActionDest: 99 Annotation: Subtype: Link Rect: 224, 340, 231, 352 ActionDest: 102 Annotation: Subtype: Link Rect: 372, 315, 379, 327 ActionDest: 99 Annotation: Subtype: Link Rect: 203, 189, 210, 201 ActionDest: 165 Annotation: Subtype: Link Rect: 261, 189, 268, 201 ActionDest: 136 Page: Sequence: 7 Page: Sequence: 8 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 416, 365, 423, 377 ActionDest: 99 Annotation: Subtype: Link Rect: 481, 353, 488, 365 ActionDest: 165 Annotation: Subtype: Link Rect: 542, 353, 549, 364 ActionDest: 165 Annotation: Subtype: Link Rect: 552, 353, 559, 364 ActionDest: 205 Page: Sequence: 10 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 506, 657, 513, 669 ActionDest: 311 Annotation: Subtype: Link Rect: 518, 441, 530, 450 ActionDest: 324 Annotation: Subtype: Link Rect: 447, 429, 459, 438 ActionDest: 324 Annotation: Subtype: Link Rect: 285, 303, 297, 312 ActionDest: 324 Annotation: Subtype: Link Rect: 301, 303, 312, 312 ActionDest: 324 Annotation: Subtype: Link Rect: 351, 300, 358, 312 ActionDest: 99 Annotation: Subtype: Link Rect: 544, 228, 556, 237 ActionDest: 324 Annotation: Subtype: Link Rect: 299, 165, 311, 174 ActionDest: 324 Annotation: Subtype: Link Rect: 252, 152, 264, 161 ActionDest: 324 Annotation: Subtype: Link Rect: 246, 140, 258, 149 ActionDest: 324 Annotation: Subtype: Link Rect: 189, 102, 201, 111 ActionDest: 324 Annotation: Subtype: Link Rect: 199, 65, 211, 73 ActionDest: 331 Annotation: Subtype: Link Rect: 513, 62, 520, 74 ActionDest: 165 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 531, 735, 543, 744 ActionDest: 331 Annotation: Subtype: Link Rect: 329, 710, 341, 719 ActionDest: 331 Annotation: Subtype: Link Rect: 497, 710, 509, 719 ActionDest: 331 Annotation: Subtype: Link Rect: 330, 697, 342, 706 ActionDest: 331 Annotation: Subtype: Link Rect: 444, 672, 456, 681 ActionDest: 331 Annotation: Subtype: Link Rect: 210, 634, 222, 643 ActionDest: 331 Annotation: Subtype: Link Rect: 258, 619, 265, 631 ActionDest: 165 Annotation: Subtype: Link Rect: 353, 609, 365, 618 ActionDest: 331 Annotation: Subtype: Link Rect: 459, 584, 471, 593 ActionDest: 331 Annotation: Subtype: Link Rect: 544, 572, 556, 581 ActionDest: 331 Annotation: Subtype: Link Rect: 207, 534, 219, 543 ActionDest: 331 Annotation: Subtype: Link Rect: 310, 531, 317, 543 ActionDest: 165 Annotation: Subtype: Link Rect: 373, 509, 384, 518 ActionDest: 331 Annotation: Subtype: Link Rect: 333, 494, 340, 506 ActionDest: 165 Annotation: Subtype: Link Rect: 531, 484, 543, 493 ActionDest: 331 Annotation: Subtype: Link Rect: 543, 484, 555, 493 ActionDest: 331 Annotation: Subtype: Link Rect: 502, 469, 509, 481 ActionDest: 165 Annotation: Subtype: Link Rect: 408, 446, 420, 455 ActionDest: 331 Annotation: Subtype: Link Rect: 264, 431, 271, 443 ActionDest: 165 Annotation: Subtype: Link Rect: 208, 408, 220, 417 ActionDest: 331 Annotation: Subtype: Link Rect: 328, 408, 340, 417 ActionDest: 331 Annotation: Subtype: Link Rect: 543, 408, 555, 417 ActionDest: 331 Annotation: Subtype: Link Rect: 247, 383, 259, 392 ActionDest: 331 Annotation: Subtype: Link Rect: 364, 371, 376, 380 ActionDest: 331 Annotation: Subtype: Link Rect: 301, 320, 313, 330 ActionDest: 331 Annotation: Subtype: Link Rect: 307, 308, 319, 317 ActionDest: 331 Annotation: Subtype: Link Rect: 319, 308, 331, 317 ActionDest: 331 Annotation: Subtype: Link Rect: 461, 308, 472, 317 ActionDest: 331 Annotation: Subtype: Link Rect: 217, 270, 229, 279 ActionDest: 331 Annotation: Subtype: Link Rect: 355, 270, 367, 279 ActionDest: 331 Annotation: Subtype: Link Rect: 485, 258, 497, 267 ActionDest: 331 Annotation: Subtype: Link Rect: 511, 217, 518, 229 ActionDest: 165 Annotation: Subtype: Link Rect: 525, 220, 537, 229 ActionDest: 334 Annotation: Subtype: Link Rect: 545, 182, 557, 192 ActionDest: 334 Annotation: Subtype: Link Rect: 242, 170, 254, 179 ActionDest: 334 Annotation: Subtype: Link Rect: 364, 82, 375, 91 ActionDest: 321 Annotation: Subtype: Link Rect: 378, 82, 390, 91 ActionDest: 321 Annotation: Subtype: Link Rect: 391, 82, 403, 91 ActionDest: 324 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 426, 105, 560, 116 Annotation: Subtype: Link Rect: 166, 93, 239, 104 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 475, 408, 512, 419 Annotation: Subtype: Link Rect: 474, 396, 512, 407 Annotation: Subtype: Link Rect: 518, 396, 554, 407 Annotation: Subtype: Link Rect: 457, 385, 495, 396 Annotation: Subtype: Link Rect: 519, 373, 557, 384 Annotation: Subtype: Link Rect: 386, 350, 423, 361 Annotation: Subtype: Link Rect: 430, 350, 466, 361 Annotation: Subtype: Link Rect: 405, 327, 443, 338 Annotation: Subtype: Link Rect: 468, 304, 505, 315 Annotation: Subtype: Link Rect: 511, 304, 547, 315 Annotation: Subtype: Link Rect: 122, 282, 160, 292 Annotation: Subtype: Link Rect: 166, 282, 202, 292 Annotation: Subtype: Link Rect: 453, 235, 490, 246 Annotation: Subtype: Link Rect: 405, 201, 442, 212 Annotation: Subtype: Link Rect: 431, 166, 468, 177 Annotation: Subtype: Link Rect: 378, 143, 416, 154 Annotation: Subtype: Link Rect: 95, 98, 133, 108 Annotation: Subtype: Link Rect: 140, 64, 178, 74 Annotation: Subtype: Link Rect: 184, 64, 220, 74 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 188, 710, 226, 720 Annotation: Subtype: Link Rect: 285, 686, 322, 697 Annotation: Subtype: Link Rect: 329, 686, 365, 697 Annotation: Subtype: Link Rect: 194, 653, 231, 663 Annotation: Subtype: Link Rect: 238, 653, 274, 663 Annotation: Subtype: Link Rect: 59, 607, 97, 617 Annotation: Subtype: Link Rect: 103, 607, 139, 617 Annotation: Subtype: Link Rect: 165, 584, 202, 594 Annotation: Subtype: Link Rect: 293, 525, 330, 536 Annotation: Subtype: Link Rect: 370, 514, 560, 525 Annotation: Subtype: Link Rect: 56, 502, 356, 513 Annotation: Subtype: Link Rect: 150, 480, 187, 490 Annotation: Subtype: Link Rect: 520, 468, 557, 479 Annotation: Subtype: Link Rect: 59, 457, 95, 467 Annotation: Subtype: Link Rect: 460, 445, 497, 456 Annotation: Subtype: Link Rect: 59, 423, 97, 433 Annotation: Subtype: Link Rect: 290, 400, 328, 410 Annotation: Subtype: Link Rect: 515, 376, 552, 387 Annotation: Subtype: Link Rect: 354, 353, 560, 364 Annotation: Subtype: Link Rect: 56, 341, 140, 352 Annotation: Subtype: Link Rect: 264, 330, 502, 341 Annotation: Subtype: Link Rect: 245, 295, 283, 306 Annotation: Subtype: Link Rect: 255, 272, 292, 283 Annotation: Subtype: Link Rect: 59, 238, 97, 249 Annotation: Subtype: Link Rect: 154, 204, 192, 214 Annotation: Subtype: Link Rect: 198, 204, 234, 214 Annotation: Subtype: Link Rect: 384, 180, 422, 191 Annotation: Subtype: Link Rect: 244, 146, 282, 157 Annotation: Subtype: Link Rect: 519, 123, 557, 134 Annotation: Subtype: Link Rect: 59, 112, 95, 122 Annotation: Subtype: Link Rect: 463, 88, 501, 99 Annotation: Subtype: Link Rect: 396, 65, 434, 76 Annotation: Subtype: Link Rect: 440, 65, 476, 76 Page: Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 375, 709, 412, 720 Annotation: Subtype: Link Rect: 519, 686, 557, 697 Annotation: Subtype: Link Rect: 263, 663, 300, 674 Annotation: Subtype: Link Rect: 299, 640, 336, 651 Annotation: Subtype: Link Rect: 104, 607, 141, 617 Annotation: Subtype: Link Rect: 378, 583, 416, 594 Annotation: Subtype: Link Rect: 519, 560, 557, 571 Annotation: Subtype: Link Rect: 59, 549, 95, 559 Annotation: Subtype: Link Rect: 59, 515, 97, 525 Annotation: Subtype: Link Rect: 491, 479, 529, 490 Annotation: Subtype: Link Rect: 95, 457, 133, 467 Annotation: Subtype: Link Rect: 59, 423, 97, 433 Annotation: Subtype: Link Rect: 171, 388, 209, 398 Annotation: Subtype: Link Rect: 215, 388, 251, 398 Annotation: Subtype: Link Rect: 345, 364, 382, 375 Annotation: Subtype: Link Rect: 519, 341, 557, 352 Annotation: Subtype: Link Rect: 270, 318, 307, 329 Annotation: Subtype: Link Rect: 320, 296, 358, 306 Annotation: Subtype: Link Rect: 59, 262, 97, 272 Annotation: Subtype: Link Rect: 190, 238, 227, 249 Annotation: Subtype: Link Rect: 204, 215, 242, 226 Annotation: Subtype: Link Rect: 457, 192, 495, 203 Annotation: Subtype: Link Rect: 501, 192, 537, 203 Annotation: Subtype: Link Rect: 389, 157, 427, 168 Annotation: Subtype: Link Rect: 389, 134, 427, 145 Annotation: Subtype: Link Rect: 433, 134, 469, 145 Annotation: Subtype: Link Rect: 127, 100, 164, 111 Annotation: Subtype: Link Rect: 171, 100, 207, 111 Annotation: Subtype: Link Rect: 59, 66, 97, 76 Page: Sequence: 17 Annotations: Annotation: Subtype: Link Rect: 520, 721, 557, 732 Annotation: Subtype: Link Rect: 216, 699, 253, 709 Annotation: Subtype: Link Rect: 317, 675, 355, 686 Annotation: Subtype: Link Rect: 361, 675, 397, 686 Checksum: 72bcb2c7 Type: CRC32 Checksum: 61cbb8502a016d4b7c2187c214635425 Type: MD5 Checksum: ca720d1e9beb64771bdfc8f3043350ef72608dfb Type: SHA-1 Checksum: 5fed767cb8fcb7e690967536a537a1520fc12f6b3b96bccb820c1701cbeb6dd5 Type: SHA-256