e4f0f92b940faf430e1d27a6d4a04534 microorganisms_a2021v9n4p703.pdf 5194acf3cb1970f692a564d5f001aab8423849a0 microorganisms_a2021v9n4p703.pdf a4b6445bc496762c205b80216970a5263fa4952e6fb61c90dc6814a38b3089b6 microorganisms_a2021v9n4p703.pdf Title: The Analysis of Live-Attenuated Piscirickettsia salmonis Vaccine Reveals the Short-Term Upregulation of Innate and Adaptive Immune Genes in Atlantic Salmon (Salmo salar): An In Situ Open-Sea Cages Study Subject: Piscirickettsia salmonis, the etiological agent of the Salmon Rickettsial Septicemia (SRS), is one the most serious health problems for the Chilean salmon industry. Typical antimicrobial strategies used against P. salmonis include antibiotics and vaccines, but these applications have largely failed. A few years ago, the first attenuated-live vaccine against SRS (ALPHA JECT LiVac® SRS vaccine) was released to the market. However, there is no data about the agents involved in the activation of the immune response induced under field conditions. Therefore, in this study we evaluated the expression profile of a set of gene markers related to innate and adaptive immunity in the context of a cellular response in Atlantic salmon (Salmo salar) reared under productive farm conditions and immunized with a live-attenuated vaccine against P. salmonis. We analyzed the expression at zero, 5-, 15- and 45-days post-vaccination (dpv). Our results reveal that the administration of the attenuated live SRS LiVac vaccine induces a short-term upregulation of the cellular-mediated immune response at 5 dpv modulated by the upregulation of ifn, ifn, and the cd4 and cd8 T cell surface markers. In addition, we also registered the upregulation of il-10 and tgf. Altogether, the results suggest that a balanced activation of the immune response took place only at early times post-vaccination (5 dpv). The scope of this short-term upregulation of the cellular-mediated immune response against a natural outbreak in fish subjected to productive farm conditions deserves further research. Keywords: Piscirickettsia salmonis; Salmonid Rickettsial Septicemia (SRS); attenuated live vaccine; cellular immunity; interferon-mediated immune response; open sea cage farming Author: Deborah Vargas, Eva Vallejos-Vidal, Sebastián Reyes-Cerpa, Aarón Oyarzún-Arrau, Claudio Acuña-Castillo, Mónica Imarai, Felipe E. Reyes-López and Ana María Sandino Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Mar 30 08:23:09 2021 CEST ModDate: Tue Mar 30 08:36:47 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 746374 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JCYIDT+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 HCRKXL+VnURWPalladioL Type 1 Custom yes yes no 15 0 NHDQNC+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 ORDUBA+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 ZUVOTZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 VICZZE+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 OCXAOJ+PazoMath-Italic Type 1 Builtin yes yes no 36 0 SIXRFS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 44 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 66 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 70 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 74 0 XEYJZU+CMSY10 Type 1 Builtin yes yes no 89 0 LEVXPH+EURM10 Type 1 Builtin yes yes no 93 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 111 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 115 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 119 0 BCDJEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 123 0 CRUZTT+VnURWPalladioL-Italic Type 1 Custom yes yes no 143 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:52:18 CEST RepresentationInformation: microorganisms_a2021v9n4p703.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:50:50 CEST Size: 746374 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 337 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Fish Destination: subsection.2.1 Item: Title: Vaccination Trial and Sampling Destination: subsection.2.2 Item: Title: RNA Extraction and cDNA Synthesis Destination: subsection.2.3 Item: Title: Gene Expression Analysis Destination: subsection.2.4 Item: Title: Statistical Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: References Destination: section.4 Info: Title: The Analysis of Live-Attenuated Piscirickettsia salmonis Vaccine Reveals the Short-Term Upregulation of Innate and Adaptive Immune Genes in Atlantic Salmon (Salmo salar): An In Situ Open-Sea Cages Study Author: Deborah Vargas, Eva Vallejos-Vidal, Sebastián Reyes-Cerpa, Aarón Oyarzún-Arrau, Claudio Acuña-Castillo, Mónica Imarai, Felipe E. Reyes-López and Ana María Sandino Subject: Piscirickettsia salmonis, the etiological agent of the Salmon Rickettsial Septicemia (SRS), is one the most serious health problems for the Chilean salmon industry. Typical antimicrobial strategies used against P. salmonis include antibiotics and vaccines, but these applications have largely failed. A few years ago, the first attenuated-live vaccine against SRS (ALPHA JECT LiVac® SRS vaccine) was released to the market. However, there is no data about the agents involved in the activation of the immune response induced under field conditions. Therefore, in this study we evaluated the expression profile of a set of gene markers related to innate and adaptive immunity in the context of a cellular response in Atlantic salmon (Salmo salar) reared under productive farm conditions and immunized with a live-attenuated vaccine against P. salmonis. We analyzed the expression at zero, 5-, 15- and 45-days post-vaccination (dpv). Our results reveal that the administration of the attenuated live SRS LiVac vaccine induces a short-term upregulation of the cellular-mediated immune response at 5 dpv modulated by the upregulation of ifn, ifn, and the cd4 and cd8 T cell surface markers. In addition, we also registered the upregulation of il-10 and tgf. Altogether, the results suggest that a balanced activation of the immune response took place only at early times post-vaccination (5 dpv). The scope of this short-term upregulation of the cellular-mediated immune response against a natural outbreak in fish subjected to productive farm conditions deserves further research. 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